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AB276441

Recombinant human IL-28B protein (His tag)

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(1 Publication)

Recombinant human IL-28B protein (His tag) is a Human Full Length protein, in the 1 to 196 aa range, expressed in HEK 293 cells, with >96%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

IL28B, IL28C, ZCYTO22, IFNL3, Interferon lambda-3, IFN-lambda-3, Cytokine Zcyto22, Interleukin-28B, Interleukin-28C, IL-28B, IL-28C

2 Images
Functional Studies - Recombinant human IL-28B protein (His tag) (AB276441)
  • FuncS

Supplier Data

Functional Studies - Recombinant human IL-28B protein (His tag) (AB276441)

Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus.

The ED50 for this effect is typically 4-16 ng/mL.

SDS-PAGE - Recombinant human IL-28B protein (His tag) (AB276441)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human IL-28B protein (His tag) (AB276441)

SDS-PAGE analysis of ab276441

Key facts

Purity

>96% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus.

The ED50 for this effect is typically 4-16 ng/mL.

Accession

Q8IZI9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 2.9% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MTGDCMPVLVLMAAVLTVTGAVPVARLRGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCKCRSRLFPRTWDLRQLQVRERPVALEAELALTLKVLEATADTDPALGDVLDQPLHTLHHILSQLRACIQPQPTAGPRTRGRLHHWLHRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV","proteinLength":"Full Length","predictedMolecularWeight":"21 kDa","actualMolecularWeight":null,"aminoAcidEnd":196,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8IZI9","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Lyophilized

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General info

Function

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

Sequence similarities

Belongs to the type-III (or lambda) interferon family.

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Product protocols

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Target data

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 623:803-813 PubMed37938781

2023

Autoantibodies against type I IFNs in humans with alternative NF-κB pathway deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Le Voyer,Audrey V Parent,Xian Liu,Axel Cederholm,Adrian Gervais,Jérémie Rosain,Tina Nguyen,Malena Perez Lorenzo,Elze Rackaityte,Darawan Rinchai,Peng Zhang,Lucy Bizien,Gonca Hancioglu,Pascale Ghillani-Dalbin,Jean-Luc Charuel,Quentin Philippot,Mame Sokhna Gueye,Majistor Raj Luxman Maglorius Renkilaraj,Masato Ogishi,Camille Soudée,Mélanie Migaud,Flore Rozenberg,Mana Momenilandi,Quentin Riller,Luisa Imberti,Ottavia M Delmonte,Gabriele Müller,Baerbel Keller,Julio Orrego,William Alexander Franco Gallego,Tamar Rubin,Melike Emiroglu,Nima Parvaneh,Daniel Eriksson,Maribel Aranda-Guillen,David I Berrios,Linda Vong,Constance H Katelaris,Peter Mustillo,Johannes Raedler,Jonathan Bohlen,Jale Bengi Celik,Camila Astudillo,Sarah Winter,Catriona McLean,Aurélien Guffroy,Joseph L DeRisi,David Yu,Corey Miller,Yi Feng,Audrey Guichard,Vivien Béziat,Jacinta Bustamante,Qiang Pan-Hammarström,Yu Zhang,Lindsey B Rosen,Steve M Holland,Marita Bosticardo,Heather Kenney,Riccardo Castagnoli,Charlotte A Slade,Kaan Boztuğ,Nizar Mahlaoui,Sylvain Latour,Roshini S Abraham,Vassilios Lougaris,Fabian Hauck,Anna Sediva,Faranaz Atschekzei,Georgios Sogkas,M Cecilia Poli,Mary A Slatter,Boaz Palterer,Michael D Keller,Alberto Pinzon-Charry,Anna Sullivan,Luke Droney,Daniel Suan,Melanie Wong,Alisa Kane,Hannah Hu,Cindy Ma,Hana Grombiříková,Peter Ciznar,Ilan Dalal,Nathalie Aladjidi,Miguel Hie,Estibaliz Lazaro,Jose Franco,Sevgi Keles,Marion Malphettes,Marlene Pasquet,Maria Elena Maccari,Andrea Meinhardt,Aydan Ikinciogullari,Mohammad Shahrooei,Fatih Celmeli,Patrick Frosk,Christopher C Goodnow,Paul E Gray,Alexandre Belot,Hye Sun Kuehn,Sergio D Rosenzweig,Makoto Miyara,Francesco Licciardi,Amélie Servettaz,Vincent Barlogis,Guillaume Le Guenno,Vera-Maria Herrmann,Taco Kuijpers,Grégoire Ducoux,Françoise Sarrot-Reynauld,Catharina Schuetz,Charlotte Cunningham-Rundles,Frédéric Rieux-Laucat,Stuart G Tangye,Cristina Sobacchi,Rainer Doffinger,Klaus Warnatz,Bodo Grimbacher,Claire Fieschi,Laureline Berteloot,Vanessa L Bryant,Sophie Trouillet Assant,Helen Su,Benedicte Neven,Laurent Abel,Qian Zhang,Bertrand Boisson,Aurélie Cobat,Emmanuelle Jouanguy,Olle Kampe,Paul Bastard,Chaim M Roifman,Nils Landegren,Luigi D Notarangelo,Mark S Anderson,Jean-Laurent Casanova,Anne Puel
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