Recombinant human IL-3RB protein (His tag) is a Human Fragment protein, in the 1 to 443 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M V L A Q G L L S M A L L A L C W E R S L A G A E E T I P L Q T L R C Y N D Y T S H I T C R W A D T Q D A Q R L V N V T L I R R V N E D L L E P V S C D L S D D M P W S A C P H P R C V P R R C V I P C Q S F V V T D V D Y F S F Q P D R P L G T R L T V T L T Q H V Q P P E P R D L Q I S T D Q D H F L L T W S V A L G S P Q S H W L S P G D L E F E V V Y K R L Q D S W E D A A I L L S N T S Q A T L G P E H L M P S S T Y V A R V R T R L A P G S R L S G R P S K W S P E V C W D S Q P G D E A Q P Q N L E C F F D G A A V L S C S W E V R K E V A S S V S F G L F Y K P S P D A G E E E C S P V L R E G L G S L H T R H H C Q I P V P D P A T H G Q Y I V S V Q P R R A E K H I K S S V N I Q M A P P S L N V T K D G D S Y S L R W E T M K M R Y E H I D H T F E I Q Y R K D T A T W K D S K T E T L Q N A H S M A L P A L E P S T R Y W A R V R V R T S R T G Y N G I W S E W S E A R S W D T E S V L P M W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA (PubMed:1495999). In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK-STAT pathway (PubMed:9516124).
Alternative names
CD131, IL3RB, IL5RB, CSF2RB, Cytokine receptor common subunit beta, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit
Recommended products
Recombinant human IL-3RB protein (His tag) is a Human Fragment protein, in the 1 to 443 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA.
Immobilized human CD131 at 10 μg/ml (100 μl/well) can bind human Fc-GMCSF.
The EC50 of human Fc-GMCSF is 250-500 ng/ml.
- Accession
- P32927-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M V L A Q G L L S M A L L A L C W E R S L A G A E E T I P L Q T L R C Y N D Y T S H I T C R W A D T Q D A Q R L V N V T L I R R V N E D L L E P V S C D L S D D M P W S A C P H P R C V P R R C V I P C Q S F V V T D V D Y F S F Q P D R P L G T R L T V T L T Q H V Q P P E P R D L Q I S T D Q D H F L L T W S V A L G S P Q S H W L S P G D L E F E V V Y K R L Q D S W E D A A I L L S N T S Q A T L G P E H L M P S S T Y V A R V R T R L A P G S R L S G R P S K W S P E V C W D S Q P G D E A Q P Q N L E C F F D G A A V L S C S W E V R K E V A S S V S F G L F Y K P S P D A G E E E C S P V L R E G L G S L H T R H H C Q I P V P D P A T H G Q Y I V S V Q P R R A E K H I K S S V N I Q M A P P S L N V T K D G D S Y S L R W E T M K M R Y E H I D H T F E I Q Y R K D T A T W K D S K T E T L Q N A H S M A L P A L E P S T R Y W A R V R V R T S R T G Y N G I W S E W S E A R S W D T E S V L P M W
- Accession
- P32927
- Protein length
- Fragment
- Predicted molecular weight
- 50 kDa
- Amino acids
- 1 to 443
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA (PubMed:1495999). In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK-STAT pathway (PubMed:9516124).
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
- Post-translational modifications
May be phosphorylated by LYN.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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2 product images
Functional Studies - Recombinant human IL-3RB protein (His tag) (ab276287)
Measured by its binding ability in a functional ELISA.
Immobilized human CD131 at 10 μg/ml (100 μl/well) can bind human Fc-GMCSF.
The EC50Â of human Fc-GMCSF is 250-500 ng/ml.
Measured by its binding ability in a functional ELISA.#Immobilized human CD131 at 10 μg/ml (100 μl/well) can bind human Fc-GMCSF.#The EC50 of human Fc-GMCSF is 250-500 ng/ml.
SDS-PAGE - Recombinant human IL-3RB protein (His tag) (ab276287)
SDS-PAGE analysis of ab276287
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