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Recombinant human IL-4 protein is a Human Full Length protein, in the 25 to 153 aa range, expressed in HEK 293, with >90% purity, < 0.1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, FuncS.
Alternative names=Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
- Purity
>90% SDS-PAGE
- Endotoxin level
< 0.1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
ELISA, SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
H K C D I T L Q E I I K T L N S L T E Q K T L C T E L T V T D I F A A S K N T T E K E T F C R A A T V L R Q F Y S H H E K D T R C L G A T A Q Q F H R H K Q L I R F L K R L D R N L W G L A G L N S C P V K E A N Q S T L E N F L E R L K T I M R E K Y S K C S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes DTT-reduced ab155733 migrates as 15 and 19 kDa protein due to glycosylation. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human IL-4 protein is a Human Full Length protein, in the 25 to 153 aa range, expressed in HEK 293, with >90% purity, < 0.1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, FuncS.
Alternative names=Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 0.1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
ELISA, SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Measured by its binding ability in a functional ELISA. Immobilized ab155733 at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL .
- Predicted molecular weight
15 kDa
- Amino acids
25 to 153
- Sequence
H K C D I T L Q E I I K T L N S L T E Q K T L C T E L T V T D I F A A S K N T T E K E T F C R A A T V L R Q F Y S H H E K D T R C L G A T A Q Q F H R H K Q L I R F L K R L D R N L W G L A G L N S C P V K E A N Q S T L E N F L E R L K T I M R E K Y S K C S S
- Accession
- P05112-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 100 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 5% Trehalose, 1.17% Sodium chloride, 0.39% MES
Specifications
- Form
Lyophilized
- Additional notes
ab155733 was lyophilised from 0.22 µm filtered solution.
General info
- Function
Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (By similarity).
- Sequence similarities
Belongs to the IL-4/IL-13 family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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