Recombinant human IL-4 protein is a Human Full Length protein, in the 25 to 153 aa range, expressed in HEK 293, with >90% purity, < 0.1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, FuncS.
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- Cancer science 114:2634-26492023LRRC75A-AS1 delivered by M2 macrophage exosomes promotes cervical cancer progression via enhancing SIX1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Ying Sui,Xiu-Ying Cui,Cai-Xia Shi,Zhi-Peng Yan,Hu-Cheng Li,Fang WangPubMed 36892427
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- ELISA, SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
H K C D I T L Q E I I K T L N S L T E Q K T L C T E L T V T D I F A A S K N T T E K E T F C R A A T V L R Q F Y S H H E K D T R C L G A T A Q Q F H R H K Q L I R F L K R L D R N L W G L A G L N S C P V K E A N Q S T L E N F L E R L K T I M R E K Y S K C S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes DTT-reduced ab155733 migrates as 15 and 19 kDa protein due to glycosylation. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed:1993171, PubMed:3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed:2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed:10219247, PubMed:11526337, PubMed:18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed:7721895).
Alternative names
Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
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Recombinant human IL-4 protein is a Human Full Length protein, in the 25 to 153 aa range, expressed in HEK 293, with >90% purity, < 0.1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- ELISA, SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized ab155733 at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL .
- Accession
- P05112-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 100 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 5% Trehalose, 1.17% Sodium chloride, 0.39% MES
Sequence info
Amino acid sequence
- H K C D I T L Q E I I K T L N S L T E Q K T L C T E L T V T D I F A A S K N T T E K E T F C R A A T V L R Q F Y S H H E K D T R C L G A T A Q Q F H R H K Q L I R F L K R L D R N L W G L A G L N S C P V K E A N Q S T L E N F L E R L K T I M R E K Y S K C S S
- Accession
- P05112
- Protein length
- Full Length
- Predicted molecular weight
- 15 kDa
- Amino acids
- 25 to 153
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
ab155733 was lyophilised from 0.22 µm filtered solution.
General info
- Function
Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed:1993171, PubMed:3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed:2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed:10219247, PubMed:11526337, PubMed:18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed:7721895).
- Sequence similarities
Belongs to the IL-4/IL-13 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Functional Studies - Recombinant human IL-4 protein (ab155733)
ab155733 stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.27-0.49 ng/mL
SDS-PAGE - Recombinant human IL-4 protein (ab155733)
Human IL-4 (Tag Free) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
ELISA - Recombinant human IL-4 protein (ab155733)
Immobilized Human IL-4 (ab155733) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL.
ELISA - Recombinant human IL-4 protein (ab155733)
ab155733 at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha (ab246188) with a linear range of 2-78 ng/mL.
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