Recombinant human IL-4 protein (Active)
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Recombinant human IL-4 protein (Active) is a Human Full Length protein, in the 25 to 153 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
View Alternative Names
Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
- FuncS (Neut/Block)
Supplier Data
Functional Studies (Neut/Block) - Recombinant human IL-4 protein (Active) (AB269147)
Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-4 (ab269147) and anti-IL-4 antibody (ab323477) or isotype IgG (ab210849).
Cell proliferation (%) (A) confirms that the anti-IL-4 antibody (ab323477) neutralizes IL-4-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 0.0164 ug/ml (B).
TF-1 cells were seeded at 5 × 10⁴ cells per well in a 96-well plate and cultured in medium containing 5% FBS for 48 h. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.
- FuncS
Unknown
Functional Studies - Recombinant human IL-4 protein (Active) (AB269147)
Biological activity graph of ab269147
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human IL-4 protein (Active) (AB269147)
SDS-PAGE analysis of ab269147 at 1ug/lane under (-) non-reducing and (+) reducing conditions. 4-20% Tris glycine gel. Stained with coomassie blue.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Lyophilized
Additional notes
nan
General info
Function
Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
Sequence similarities
Belongs to the IL-4/IL-13 family.
Target data
Complementary Products
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AB259377
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