JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB225862

Recombinant human IL-4 Receptor alpha protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human IL-4 Receptor alpha protein is a Human Fragment protein, in the 24 to 232 aa range, expressed in CHO cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The ED50 was determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of IL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.

Accession

P24394

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"GNMKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH","proteinLength":"Fragment","predictedMolecularWeight":"23.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":232,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"P24394","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column
style
left-column

Specifications

Form

Lyophilized

Additional notes

Greater than 97% by SDS-PAGE and HPLC analyses.

style
left-column

General info

Function

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 4 subfamily.

Post-translational modifications

On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.. The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.

style
left-column

Product protocols

style
left-column

Target data

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
See full target information IL4R
style
left-column
style
left-column
style
right-column

Alternative Products

Proteins & Peptides

AB208322

Recombinant Cynomolgus monkey IL-4 Receptor alpha protein (Fc Chimera)

ELISA - Recombinant Cynomolgus monkey IL-4 Receptor alpha protein (Fc Chimera) (AB208322)

  • 0
  • 0
Proteins & Peptides

AB158758

Recombinant Human IL-4 Receptor alpha protein

SDS-PAGE - Recombinant Human IL-4 Receptor alpha protein (AB158758)

  • 0
  • 0

Complementary Products

Proteins & Peptides

AB259406

Recombinant mouse IL-4 protein (Active)

Premium Bioactive

Functional Studies - Recombinant mouse IL-4 protein (Active) (AB259406)

  • 0
  • 0
Primary Antibodies

AB259498

Anti-IL-4R antibody [EPR21947-278] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-4R antibody [EPR21947-278] - BSA and Azide free (Detector) (AB259498)

  • 0
  • 0
Proteins & Peptides

AB307475

Recombinant Human CD86 protein (Fc Chimera)

Mass Spectrometry - Recombinant Human CD86 protein (Fc Chimera) (AB307475)

  • 0
  • 0
Proteins & Peptides

AB259402

Recombinant human IL-10 protein (Active)

Premium Bioactive

Functional Studies - Recombinant human IL-10 protein (Active) (AB259402)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com