Recombinant Human IL-4R Protein Standard (His tag)
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Recombinant Human IL-4R Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
View Alternative Names
CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Human IL-4R Protein Standard (His tag) (AB316645)
Sandwich ELISA with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human IL-4R Protein Standard (His tag) (AB316645)
SDS-PAGE analysis of ab316645 under reducing conditions for 2ug protein
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
General info
Function
Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Post-translational modifications
On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.. The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
Target data
Complementary Products
Primary Antibodies
AB259498
Anti-IL-4R antibody [EPR21947-278] - BSA and Azide free (Detector)
RabMAb|Recombinant
![Sandwich ELISA - Anti-IL-4R antibody [EPR21947-278] - BSA and Azide free (Detector) (AB259498)](https://content.abcam.com/products/images/il-4r-antibody-epr21947-278-bsa-and-azide-free-detector-ab259498--sandwich-elisa-img138642.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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