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AB283427

Recombinant Human IL-5 protein (Active)

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Recombinant Human IL-5 protein (Active) is a Human Full Length protein, in the 20 to 134 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5

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Mass Spectrometry - Recombinant Human IL-5 protein (Active) (AB283427)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Human IL-5 protein (Active) (AB283427)

Mass determination of ab283427 by ESI-TOF.

Predicted MW is 13206.27 Da (+/- 10 Da by ESI-TOF). Observed MW is 13208.40 Da. Additional masses at 13411.42 Da and 13776.69 Da are due to residual O-glycans.

Functional Studies - Recombinant Human IL-5 protein (Active) (AB283427)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human IL-5 protein (Active) (AB283427)

Fully biologically active determined by the dose dependent proliferation of TF-1 cells.  ED50 is ≤ 0.15 ng/ml, corresponding to a specific activity of 6.9 x 10⁶ units/mg.  Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3398596-2.

SDS-PAGE - Recombinant Human IL-5 protein (Active) (AB283427)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant Human IL-5 protein (Active) (AB283427)

SDS-PAGE analysis of ab283427.

HPLC - Recombinant Human IL-5 protein (Active) (AB283427)
  • HPLC

Lab

HPLC - Recombinant Human IL-5 protein (Active) (AB283427)

HPLC analysis of ab283427.

Key facts

Purity

>95% SDS-PAGE

>95% purity by HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, SDS-PAGE, FuncS, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent proliferation of TF-1 cells.

ED50 is ≤ 0.15 ng/ml, corresponding to a specific activity of 6.9 x 106 units/mg.

Accession

P05113

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES","proteinLength":"Full Length","predictedMolecularWeight":"13.21 kDa","actualMolecularWeight":"13.21 kDa","aminoAcidEnd":134,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P05113","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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General info

Function

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 2653458, PubMed : 9010276). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed : 1495999, PubMed : 22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed : 7613138).

Sequence similarities

Belongs to the IL-5 family.

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Product protocols

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Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 2653458, PubMed : 9010276). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed : 1495999, PubMed : 22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed : 7613138).
See full target information IL5
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Product promise

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For full details, please see our Terms & Conditions

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