Recombinant Human IL-5RA protein is a Human Fragment protein, in the 21 to 335 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
D L L P D E K I S L L P P V N F T I K V T G L A Q V L L Q W K P N P D Q E Q R N V N L E Y Q V K I N A P K E D D Y E T R I T E S K C V T I L H K G F S A S V R T I L Q N D H S L L A S S W A S A E L H A P P G S P G T S I V N L T C T T N T T E D N Y S R L R S Y Q V S L H C T W L V G T D A P E D T Q Y F L Y Y R Y G S W T E E C Q E Y S K D T L G R N I A C W F P R T F I L S K G R D W L A V L V N G S S K H S A I R P F D Q L F A L H A I D Q I N P P L N V T A E I E G T R L S I Q W E K P V S A F P I H C F D Y E V K I H N T R N G Y L Q I E K L M T N A F I S I I D D L S K Y D V Q V R A A V S S M C R E A G L W S E W S Q P I Y V G N D E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
This is the receptor for interleukin-5. The alpha chain binds to IL5.
Alternative names
Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, IL5R, IL5RA
Recommended products
Recombinant Human IL-5RA protein is a Human Fragment protein, in the 21 to 335 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, IL5R, IL5RA
Key facts
- Purity
>85% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q01344-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
D L L P D E K I S L L P P V N F T I K V T G L A Q V L L Q W K P N P D Q E Q R N V N L E Y Q V K I N A P K E D D Y E T R I T E S K C V T I L H K G F S A S V R T I L Q N D H S L L A S S W A S A E L H A P P G S P G T S I V N L T C T T N T T E D N Y S R L R S Y Q V S L H C T W L V G T D A P E D T Q Y F L Y Y R Y G S W T E E C Q E Y S K D T L G R N I A C W F P R T F I L S K G R D W L A V L V N G S S K H S A I R P F D Q L F A L H A I D Q I N P P L N V T A E I E G T R L S I Q W E K P V S A F P I H C F D Y E V K I H N T R N G Y L Q I E K L M T N A F I S I I D D L S K Y D V Q V R A A V S S M C R E A G L W S E W S Q P I Y V G N D E
- Accession
- Protein length
Fragment
- Predicted molecular weight
37.7 kDa
- Amino acids
21 to 335
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
This is the receptor for interleukin-5. The alpha chain binds to IL5.
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
- Activity summary
IL-5RA also known as the interleukin-5 receptor alpha chain or IL-5 receptor is a protein with a critical role in the immune system. It is also referred to as CD125 or CaL40. The protein has an approximate mass of 52 kDa and is expressed mainly on eosinophils and basophils which are types of white blood cells involved in immune responses. The IL-5RA forms a part of the IL-5 receptor complex which also includes a beta chain responsible for signal transduction.
- Biological function summary
IL-5RA plays an important role in the growth differentiation and activation of eosinophils. The protein forms part of the IL-5 receptor complex which binds to the cytokine IL-5 initiating a signaling cascade that results in these processes. Through its interaction with IL-5 IL-5RA influences the survival and function of eosinophils cells critical for responses against parasitic infections and in allergic reactions.
- Pathways
IL-5RA is fundamental in the JAK-STAT signaling pathway. This pathway is significant as it facilitates the transduction of extracellular cytokine signals into cellular responses impacting the growth and activity of eosinophils. The IL-5 receptor complex is associated with other cytokine receptors through this pathway involving proteins such as JAK2 and STAT5 which play essential roles in the activation of downstream signaling events and cellular responses.
- Associated diseases and disorders
IL-5RA is linked with asthma and eosinophilic esophagitis conditions characterized by an abnormal proliferation and activation of eosinophils. In asthma for example the increased activity of IL-5RA leads to elevated levels of eosinophils contributing to airway inflammation and hyperresponsiveness. IL-5RA also connects with other proteins like IL-5 itself serving as a therapeutic target in treatments aimed at regulating excessive eosinophil activity in these disorders.
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1 product image
SDS-PAGE - Recombinant Human IL-5RA protein (ab221341)
SDS-PAGE (DTT reduced) analysis of ab221341 followed by staining overnight with Coomassie Blue. The reduced protein migrates as 45-55 kDa in SDS-PAGE due to glycosylation.
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