Recombinant Human IL-5RA protein is a Human Fragment protein, in the 21 to 335 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
D L L P D E K I S L L P P V N F T I K V T G L A Q V L L Q W K P N P D Q E Q R N V N L E Y Q V K I N A P K E D D Y E T R I T E S K C V T I L H K G F S A S V R T I L Q N D H S L L A S S W A S A E L H A P P G S P G T S I V N L T C T T N T T E D N Y S R L R S Y Q V S L H C T W L V G T D A P E D T Q Y F L Y Y R Y G S W T E E C Q E Y S K D T L G R N I A C W F P R T F I L S K G R D W L A V L V N G S S K H S A I R P F D Q L F A L H A I D Q I N P P L N V T A E I E G T R L S I Q W E K P V S A F P I H C F D Y E V K I H N T R N G Y L Q I E K L M T N A F I S I I D D L S K Y D V Q V R A A V S S M C R E A G L W S E W S Q P I Y V G N D E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:9378992). Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed:1495999, PubMed:22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed:9516124).
Alternative names
CD125, IL5R, IL5RA, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125
Recommended products
Recombinant Human IL-5RA protein is a Human Fragment protein, in the 21 to 335 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q01344-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- D L L P D E K I S L L P P V N F T I K V T G L A Q V L L Q W K P N P D Q E Q R N V N L E Y Q V K I N A P K E D D Y E T R I T E S K C V T I L H K G F S A S V R T I L Q N D H S L L A S S W A S A E L H A P P G S P G T S I V N L T C T T N T T E D N Y S R L R S Y Q V S L H C T W L V G T D A P E D T Q Y F L Y Y R Y G S W T E E C Q E Y S K D T L G R N I A C W F P R T F I L S K G R D W L A V L V N G S S K H S A I R P F D Q L F A L H A I D Q I N P P L N V T A E I E G T R L S I Q W E K P V S A F P I H C F D Y E V K I H N T R N G Y L Q I E K L M T N A F I S I I D D L S K Y D V Q V R A A V S S M C R E A G L W S E W S Q P I Y V G N D E
- Accession
- Q01344
- Protein length
- Fragment
- Predicted molecular weight
- 37.7 kDa
- Amino acids
- 21 to 335
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:9378992). Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed:1495999, PubMed:22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed:9516124).
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IL-5RA also known as the interleukin-5 receptor alpha chain or IL-5 receptor is a protein with a critical role in the immune system. It is also referred to as CD125 or CaL40. The protein has an approximate mass of 52 kDa and is expressed mainly on eosinophils and basophils which are types of white blood cells involved in immune responses. The IL-5RA forms a part of the IL-5 receptor complex which also includes a beta chain responsible for signal transduction.
- Biological function summary
IL-5RA plays an important role in the growth differentiation and activation of eosinophils. The protein forms part of the IL-5 receptor complex which binds to the cytokine IL-5 initiating a signaling cascade that results in these processes. Through its interaction with IL-5 IL-5RA influences the survival and function of eosinophils cells critical for responses against parasitic infections and in allergic reactions.
- Pathways
IL-5RA is fundamental in the JAK-STAT signaling pathway. This pathway is significant as it facilitates the transduction of extracellular cytokine signals into cellular responses impacting the growth and activity of eosinophils. The IL-5 receptor complex is associated with other cytokine receptors through this pathway involving proteins such as JAK2 and STAT5 which play essential roles in the activation of downstream signaling events and cellular responses.
- Associated diseases and disorders
IL-5RA is linked with asthma and eosinophilic esophagitis conditions characterized by an abnormal proliferation and activation of eosinophils. In asthma for example the increased activity of IL-5RA leads to elevated levels of eosinophils contributing to airway inflammation and hyperresponsiveness. IL-5RA also connects with other proteins like IL-5 itself serving as a therapeutic target in treatments aimed at regulating excessive eosinophil activity in these disorders.
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1 product image
SDS-PAGE - Recombinant Human IL-5RA protein (ab221341)
SDS-PAGE (DTT reduced) analysis of ab221341 followed by staining overnight with Coomassie Blue. The reduced protein migrates as 45-55 kDa in SDS-PAGE due to glycosylation.
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