Name: Recombinant human IL-6 protein (Active)
SKU: ab259381
Rating: 5 (1 reviews)

Recombinant human IL-6 protein (Active) is a Human Full Length protein, in the 28 to 212 aa range with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functinonal studies, ELISA, Cell Culture and more. The predicted molecular weight of ab259381 protein is 21 kDa.

- Save time and ensure accurate results - use our IL-6 protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs

Images

Sandwich ELISA - Recombinant human IL-6 protein (Active) (AB259381), expandable thumbnail

Publications

Key facts

Purity: >=95% SDS-PAGE
Endotoxin level: < 0.005 EU/µg
Expression system: HEK 293 cells
Tags: Tag free
Applications: SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture
Biologically active: Yes

Amino acid sequence

A P V P P G E D S K D V A A P H R Q P L T S S E R I D K Q I R Y I L D G I S A L R K E T C N K S N M C E S S K E A L A E N N L N L P K M A E K D G C F Q S G F N E E T C L V K I I T G L L E F E V Y L E Y L Q N R F E S S E E Q A R A V Q M S T K V L I Q F L Q K K A K N L D A I T T P D P T T N A S L L T K L Q A Q N Q W L Q D M T T H L I L R S F K E F L Q S S L R A L R Q M

Reactivity data

Application	Reactivity	Dilution info	Notes
Application SDS-PAGE	Reactivity Reacts	Dilution info -	Notes -
Application FuncS	Reactivity Reacts	Dilution info -	Notes -
Application MS	Reactivity Reacts	Dilution info -	Notes -
Application HPLC	Reactivity Reacts	Dilution info -	Notes -
Application sELISA	Reactivity Reacts	Dilution info -	Notes -
Application Cell Culture	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information IL6

Function

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells (By similarity). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability (PubMed:12794819, PubMed:17075861). Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed:12794819). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed:20823453). 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis (By similarity). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (By similarity). Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed:15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).

Alternative names

IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2

Key facts

Purity: >=95% SDS-PAGE
Endotoxin level: < 0.005 EU/µg
Expression system: HEK 293 cells
Tags: Tag free
Applications: SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture
Biologically active: Yes
Biological activity: Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependant Proliferation of TF-1 cells is 0.8067 ng/mL corresponding to a specific activity of 1.24 x 10⁶ IU/mg.
Accession: P05231-1
Animal free: Yes
Species: Human
Reconstitution: Reconstitute in PBS
Concentration
Storage buffer: pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

A P V P P G E D S K D V A A P H R Q P L T S S E R I D K Q I R Y I L D G I S A L R K E T C N K S N M C E S S K E A L A E N N L N L P K M A E K D G C F Q S G F N E E T C L V K I I T G L L E F E V Y L E Y L Q N R F E S S E E Q A R A V Q M S T K V L I Q F L Q K K A K N L D A I T T P D P T T N A S L L T K L Q A Q N Q W L Q D M T T H L I L R S F K E F L Q S S L R A L R Q M
Accession: P05231
Protein length: Full Length
Predicted molecular weight: 21.04 kDa
Amino acids: 28 to 212
Nature: Recombinant

Specifications

Form: Lyophilized
Additional notes: Purity by HPLC >=95%.

General info

Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).
Sequence similarities: Belongs to the IL-6 superfamily.
Post-translational modifications: N- and O-glycosylated.

Storage

Shipped at conditions: Ambient - Can Ship with Ice
Appropriate short-term storage conditions: Ambient
Appropriate long-term storage conditions: Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Ensure the validity of your result using our recombinant human IL-6 protein ab259381 as a control.

The ab259381 IL-6 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC. Analyze your IL-6 ELISA data using the ab259381 protein to generate and plot a standard curve.

Check out our protein gel staining guide for SDS-PAGE here

Check out our ELISA protocol for more information here.

Premium Bioactive Protein range

The ab259381 IL-6 protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.

More premium bioactive proteins can be found here

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.

Biological function summary

IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.

Pathways

IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.

Associated diseases and disorders

IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.

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8 product images

Sandwich ELISA - Recombinant human IL-6 protein (Active) (ab259381)
Sandwich ELISA - Recombinant human IL-6 protein standard curve.
Background subtracted standard curve using Human IL-6 Antibody Pair - BSA and Azide free (Human/Monkey IL-6 Antibody Pair - BSA and Azide free ab243973) and Recombinant human IL-6 protein (Active) (ab259381) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
Functional Studies - Recombinant human IL-6 protein (Active) (ab259381)
Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependant Proliferation of TF-1 cells is 0.8067 ng/mL corresponding to a specific activity of 1.24 x 10⁶ IU/mg.
SDS-PAGE - Recombinant human IL-6 protein (Active) (ab259381)
SDS-PAGE analysis of ab259381.
HPLC - Recombinant human IL-6 protein (Active) (ab259381)
Purity 99%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Mass Spectrometry - Recombinant human IL-6 protein (Active) (ab259381)
M+1 Da, pass (+203 Da: Hex, +203: HexNAc, +291:NeuAc).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
Functional Studies - Recombinant human IL-6 protein (Active) (ab259381)
Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-6 (ab259381) and anti-IL-6R antibody (Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin ab323483) or isotype IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free ab210849).
Cell proliferation (%) (A) confirms that the anti-IL-6R antibody (Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin ab323483) neutralizes IL-6-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 0.1914 ug/ml (B).
TF-1 cells were plated at a density of 5 × 10⁴ cells per well in a 96-well plate. A dilution series of the anti-IL-6R antibody (Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin ab323483) was added to the cells and pre-incubated for 2 hours before IL-6 (ab259381) stimulation, and cells were cultured in medium with 5% FBS for a duration of 48 hours. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.
Functional Studies - Recombinant human IL-6 protein (Active) (ab259381)
Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-6 (ab259381) and anti-IL-6 antibody (Anti-IL-6 antibody [EPRNGS_IL-6-10] - BSA and Azide free, low endotoxin ab322419) or isotype IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free ab210849).
Cell proliferation (%) (A) confirms that the anti-IL-6 antibody (Anti-IL-6 antibody [EPRNGS_IL-6-10] - BSA and Azide free, low endotoxin ab322419) neutralizes IL-6-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 1.450 μg/ml (B).
TF-1 cells were seeded at 5 × 10⁴ cells per well in a 96-well plate and cultured in medium containing 5% FBS for 48 h. Luminescence was recorded using a Microplate Reader at 560nm emission.
Functional Studies - Recombinant human IL-6 protein (Active) (ab259381)
Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-6 (ab259381) and anti-IL-6 antibody (Anti-IL-6- antibody [EPRNGS_IL-6-20] BSA and Azide free, low endotoxin ab323372) or isotype IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free ab210849).
Cell proliferation (%) (A) confirms that the anti-IL-6 antibody (Anti-IL-6- antibody [EPRNGS_IL-6-20] BSA and Azide free, low endotoxin ab323372) neutralizes IL-6-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 4.990 ng/ml (B).
TF-1 cells were seeded at 5 × 10⁴ cells per well in a 96-well plate and cultured in medium containing 5% FBS for 48 h. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.