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Recombinant human IL-6 protein (Active) is a Human Full Length protein, in the 28 to 212 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture.

Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture

Biologically active

Yes

Sequence

A P V P P G E D S K D V A A P H R Q P L T S S E R I D K Q I R Y I L D G I S A L R K E T C N K S N M C E S S K E A L A E N N L N L P K M A E K D G C F Q S G F N E E T C L V K I I T G L L E F E V Y L E Y L Q N R F E S S E E Q A R A V Q M S T K V L I Q F L Q K K A K N L D A I T T P D P T T N A S L L T K L Q A Q N Q W L Q D M T T H L I L R S F K E F L Q S S L R A L R Q M

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human IL-6 protein (Active) is a Human Full Length protein, in the 28 to 212 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Proliferation of TF-1 cells is 0.8067 ng/mL corresponding to a specific activity of 1.24 x 106 IU/mg.

Predicted molecular weight

21.04 kDa

Amino acids

28 to 212

Sequence

A P V P P G E D S K D V A A P H R Q P L T S S E R I D K Q I R Y I L D G I S A L R K E T C N K S N M C E S S K E A L A E N N L N L P K M A E K D G C F Q S G F N E E T C L V K I I T G L L E F E V Y L E Y L Q N R F E S S E E Q A R A V Q M S T K V L I Q F L Q K K A K N L D A I T T P D P T T N A S L L T K L Q A Q N Q W L Q D M T T H L I L R S F K E F L Q S S L R A L R Q M

Accession
P05231-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

Purity by HPLC >=95%.

General info

Function

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).

Sequence similarities

Belongs to the IL-6 superfamily.

Post-translational modifications

N- and O-glycosylated.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilized prior to aliquoting and lyophilization. All aliquoting and lyophilization steps are performed in a sterile environment

Product promise

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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