Recombinant human IL-8 protein (Active) is a Human Full Length protein in the 29 to 99 aa range with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, mass spectrometry, HPLC and Cell Culture. The predicted molecular weight of ab259397 protein is 8.36 kDa.
- Save time and ensure accurate results - use our recombinant IL-8 protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
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Publications
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- Nature communications 15:6912024Exosomal secreted SCIMP regulates communication between macrophages and neutrophils in pneumonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolei Pei,Li Liu,Jieru Wang,Changyuan Guo,Qingqing Li,Jia Li,Qian Ren,Runzhi Ma,Yi Zheng,Yan Zhang,Li Liu,Danfeng Zheng,Pingzhang Wang,Ping Jiang,Xiaoming Feng,Erlie Jiang,Ying Wang,Sizhou FengPubMed 38263143
- Biomedical optics express 15:185-1982024Functionalized optical fiber ball-shaped biosensor for label-free, low-limit detection of IL-8 protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyunel Rashidova,Meruyert Tilegen,Tri T Pham,Aliya Bekmurzayeva,Daniele TosiPubMed 38223184
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
A K E L R C Q C I K T Y S K P F H P K F I K E L R V I E S G P H C A N T E I I V K L S D G R E L C L D P K E N W V Q R V V E K F L K R A E N S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).
Alternative names
IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1
Recommended products
Recombinant human IL-8 protein (Active) is a Human Full Length protein in the 29 to 99 aa range with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, mass spectrometry, HPLC and Cell Culture. The predicted molecular weight of ab259397 protein is 8.36 kDa.
- Save time and ensure accurate results - use our recombinant IL-8 protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active as determined by the dose dependant migration of human PBMCs.
ED50 is ≤ 12 ng/mL, corresponding to a specific activity of 8.33 x 104 units/mg.
- Accession
- P10145-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A K E L R C Q C I K T Y S K P F H P K F I K E L R V I E S G P H C A N T E I I V K L S D G R E L C L D P K E N W V Q R V V E K F L K R A E N S
- Accession
- P10145
- Protein length
- Full Length
- Predicted molecular weight
- 8.36 kDa
- Amino acids
- 29 to 99
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).
- Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family.
- Post-translational modifications
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our recombinant human IL-8 protein ab259397 as a control.
The ab259397 IL-8 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.
Check out our protein gel staining guide for SDS-PAGE here
Premium Bioactive Protein range
The ab259397 IL-8 protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
More premium bioactive proteins can be found here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.
- Biological function summary
IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.
- Pathways
IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.
- Associated diseases and disorders
IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Functional Studies - Recombinant human IL-8 protein (Active) (ab259397)
Fully biologically active as determined by the dose dependant migration of human PBMCs.
ED50 is ≤ 12 ng/mL, corresponding to a specific activity of 8.33 x 104 units/mg.
SDS-PAGE - Recombinant human IL-8 protein (Active) (ab259397)
SDS-PAGE analysis of ab259397.
HPLC - Recombinant human IL-8 protein (Active) (ab259397)
Purity = 100%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water: TFA (99.9:0.1 v/v) and 20 % acetonitrile:waterTFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Mass Spectrometry - Recombinant human IL-8 protein (Active) (ab259397)
M +/- 0 Da (Calc mass 8356 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
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