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AB290103

Recombinant Human IL1R1 Protein (Active)

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Recombinant Human IL1R1 Protein (Active) is a Human Fragment protein, in the 21 to 338 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, Mass Spec, FuncS.

View Alternative Names

CD121a, IL1R, IL1RA, IL1RT1, IL1R1, Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, Interleukin-1 receptor type I, p80, IL-1R-alpha

4 Images
Biological Activity - Recombinant Human IL1R1 Protein (Active) (AB290103)
  • Biological Activity

Supplier Data

Biological Activity - Recombinant Human IL1R1 Protein (Active) (AB290103)

Fully biologically active determined by its ability to inhibit IL-1 beta dependent proliferation of D10S cells. The ED50 ≤ 3.8 ug/ml, corresponding to a specific activity of 2.6 x 103 units/mg.

Mass Spectrometry - Recombinant Human IL1R1 Protein (Active) (AB290103)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human IL1R1 Protein (Active) (AB290103)

Mass determination by ESI-TOF.

Predicted MW is 35920.91 Da. (+/- 10 Da by ESI-TOF). Observed MW is 35927.89 Da.

SDS-PAGE - Recombinant Human IL1R1 Protein (Active) (AB290103)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IL1R1 Protein (Active) (AB290103)

SDS-PAGE analysis of ab290103

HPLC - Recombinant Human IL1R1 Protein (Active) (AB290103)
  • HPLC

Supplier Data

HPLC - Recombinant Human IL1R1 Protein (Active) (AB290103)

HPLC analysis of ab290103

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, Mass Spec, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by its ability to inhibit IL-1 beta dependent proliferation of D10S cells. The ED50 ≤ 3.8 ug/ml, corresponding to a specific activity of 2.6 x 103 units/mg.

Accession

P14778

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"DKCKEREEKIILVSSANEIDVRPCPLNPNEHKGTITWYKDDSKTPVSTEQASRIHQHKEKLWFVPAKVEDSGHYYCVVRNSSYCLRIKISAKFVENEPNLCYNAQAIFKQKLPVAGDGGLVCPYMEFFKNENNELPKLQWYKDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASYTYLGKQYPITRVIEFITLEENKPTRPVIVSPANETMEVDLGSQIQLICNVTGQLSDIAYWKWNGSVIDEDDPVLGEDYYSVENPANKRRSTLITVLNISEIESRFYKHPFTCFAKNTHGIDAAYIQLIYPVT","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":338,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P14778","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IL-1 Receptor I also known as IL-1R1 is a transmembrane protein with alternative names such as IL-1 receptor and IL-1 type I receptor. It acts as an important receptor for the pro-inflammatory cytokine Interleukin-1 (IL-1) family primarily IL-1α and IL-1β. IL-1R1 has a mass of approximately 80 kilodaltons and is expressed in a variety of cell types including endothelial cells epithelial cells and fibroblasts. It is also present on immune cells like monocytes and macrophages making it widespread in a vast range of tissues.
Biological function summary

IL-1R1 is a critical player in the initiation of immune responses serving as a component of the IL-1 signaling complex. When IL-1 binds to IL-1R1 it recruits the IL-1 receptor accessory protein (IL-1RAcP) to form a signaling complex that triggers downstream pathways. This interaction initiates a signaling cascade that activates nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) promoting the expression of inflammatory genes and mediating immune and inflammatory responses.

Pathways

IL-1R1 is central to the inflammatory response and immune signaling pathways. The IL-1 receptor complex facilitates the activation of NF-κB which plays a role in the body's response to stress cytokines and pathogens. The receptor acts in close relation with other proteins like IL-1RAcP and IRAKs (Interleukin-1 receptor-associated kinases) in these pathways creating a robust immune signaling network that drives the inflammatory response.

IL-1R1 is closely associated with inflammatory conditions such as rheumatoid arthritis and inflammatory bowel disease. Increased IL-1R1 expression and signaling contribute to chronic inflammation in these diseases. The interaction of IL-1β with IL-1R1 and subsequently with proteins like NF-κB leads to the persistence of inflammatory signals. Moreover targeting IL-1R1 and related proteins offers potential therapeutic strategies in mitigating the symptoms and progression of inflammatory disorders.
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Specifications

Form

Lyophilized

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General info

Function

Receptor for IL1A, IL1B and IL1RN (PubMed : 2950091, PubMed : 37315560). After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (PubMed : 10653850).

Sequence similarities

Belongs to the interleukin-1 receptor family.

Post-translational modifications

A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding).. Rapidly phosphorylated on Tyr-496 in response to IL-1, which creates a SH2 binding site for the PI 3-kinase regulatory subunit PIK3R1.

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Product protocols

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Target data

Receptor for IL1A, IL1B and IL1RN (PubMed : 2950091, PubMed : 37315560). After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (PubMed : 10653850).
See full target information IL1R1
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