Recombinant human IL2 Receptor beta/p75 protein (Active) is a Human Fragment protein, in the 27 to 239 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for ELISA, Biological Activity, SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- ELISA, Biological Activity, SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A V N G T S Q F T C F Y N S R A N I S C V W S Q D G A L Q D T S C Q V H A W P D R R R W N Q T C E L L P V S Q A S W A C N L I L G A P D S Q K L T T V D I V T L R V L C R E G V R W R V M A I Q D F K P F E N L R L M A P I S L Q V V H V E T H R C N I S W E I S Q A S H Y F E R H L E F E A R T L S P G H T W E E A P L L T L K Q K Q E W I C L E T L T P D T Q Y E F Q V R V K P L Q G E F T T W S P W S Q P L A F R T K P A A L G K D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application Biological Activity | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes DTT-reduced Protein migrates as 40 kDa due to glycosylation. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed:15123770, PubMed:31040185).
Alternative names
CD122, IL15RB, IL2RB, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, p70-75, p75
Recommended products
Recombinant human IL2 Receptor beta/p75 protein (Active) is a Human Fragment protein, in the 27 to 239 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for ELISA, Biological Activity, SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- ELISA, Biological Activity, SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in SPR assay. ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 0.525 μM.
Measured by its binding ability in SPR assay. ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-15, Tag Free with an affinity constant of 21.7 nM.
Measured by its binding ability in BLI assay. Loaded ab174003 on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e).
Measured by its inhibitory action in a cell based assay. ab174003 inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 μg/mL.
- Accession
- P14784-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- A V N G T S Q F T C F Y N S R A N I S C V W S Q D G A L Q D T S C Q V H A W P D R R R W N Q T C E L L P V S Q A S W A C N L I L G A P D S Q K L T T V D I V T L R V L C R E G V R W R V M A I Q D F K P F E N L R L M A P I S L Q V V H V E T H R C N I S W E I S Q A S H Y F E R H L E F E A R T L S P G H T W E E A P L L T L K Q K Q E W I C L E T L T P D T Q Y E F Q V R V K P L Q G E F T T W S P W S Q P L A F R T K P A A L G K D
- Accession
- P14784
- Protein length
- Fragment
- Predicted molecular weight
- 25.4 kDa
- Amino acids
- 27 to 239
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed:15123770, PubMed:31040185).
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Reconstitute for long term storage
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as IL2 Receptor beta.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IL2 Receptor beta also known as IL-2R beta IL2R beta or CD122/IL2RB protein is a component of the interleukin-2 receptor complex. It is part of the receptor system for the cytokine IL-2. This receptor subunit has a molecular mass of approximately 75 kDa and gets alternatively referred to as IL2R beta or p75. It is expressed on the surface of activated T cells natural killer (NK) cells and in some cases myeloid lineage cells. This expression pattern correlates IL2R beta's role in essential immune functions and signaling processes.
- Biological function summary
IL2R beta plays a critical role in cytokine binding and signal transduction for the interleukin-2 receptor which is a heterodimeric complex. This complex consists of the IL-2 receptor alpha (CD25) IL-2 receptor beta and a common gamma chain (γc CD132). The presence of IL-2R beta allows the receptor to mediate biological processes like cell proliferation differentiation and survival particularly within the immune system. The extent to how IL2R beta integrates into these processes makes it an important player in immune regulation and homeostasis.
- Pathways
IL2R beta serves essential functions in the JAK-STAT and PI3K-AKT pathways. The binding of IL-2 to its receptor initiates a cascade of intracellular events which involve Janus kinases (JAKs) and signal transducer and activator of transcription (STATs) leading to transcriptional activation of various genes. Along with these pathways IL2R beta also interacts collaboratively with the gamma chain (γc) in promoting Downstream signaling events which enhance cell proliferation and survival.
- Associated diseases and disorders
IL2R beta's function has implications in autoimmune diseases and certain types of leukemia. Alterations or dysregulation in IL-2R signaling pathways often play a role in autoimmune conditions due to its importance in maintaining immune tolerance. Moreover given IL-2 receptor's role in T-cell and NK-cell activity aberrations may contribute to uncontrolled cellular proliferation seen in leukemia. Understanding its interplay with the common gamma chain (CD132) can provide insights into therapeutic interventions targeting these disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Biological Activity - Recombinant human IL2 Receptor beta/p75 protein (Active) (ab174003)
ab174003 inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 μg/mL.
Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (Active) (ab174003)
Loaded ab174003 on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e).
Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (Active) (ab174003)
ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-15, Tag Free with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore T200).
Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (Active) (ab174003)
ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 0.525 μM as determined in a SPR assay (Biacore T200).
SDS-PAGE - Recombinant human IL2 Receptor beta/p75 protein (Active) (ab174003)
SDS-PAGE of reduced ab174003 stained overnight with Coomassie Blue. The protein migrates as 35-45 kDa due to glycosylation.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com