Recombinant Human IL3RA/CD123 protein (Fc Chimera)
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(1 Publication)
Recombinant Human IL3RA/CD123 protein (Fc Chimera) is a Human Fragment protein, in the 19 to 302 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for FuncS, SDS-PAGE.
View Alternative Names
CD123, IL3R, IL3RA, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA
- FuncS
Unknown
Functional Studies - Recombinant Human IL3RA/CD123 protein (Fc Chimera) (AB88358)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MWt and pI of the protein.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human IL3RA/CD123 protein (Fc Chimera) (AB88358)
1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 : ab88358
Lane 2 : ab88358 treated with PNGase F to remove potential N-linked glycans
Lane 3 : ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human IL3RA/CD123 protein (Fc Chimera) (AB88358)
A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358.
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Reactivity data
Product details
Previously labelled as IL3RA.
Molecular Mass: ab88358 migrates as a broad band between 85 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 60.6 kDa.
ab88358 consists of 25-40% carbohydrate by weight. pI: ab88358 separates into a number of isoforms with a pI between 6.2 and 9.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 8.43. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IL3RA forms part of a heterodimeric receptor complex binding with the beta-common (βc) chain to create the high-affinity IL-3 receptor. This complex plays a role in mediating the effects of interleukin-3 a cytokine that promotes the growth and differentiation of pluripotent hematopoietic stem cells. Through this mechanism the receptor influences immune response modulation particularly in allergic reactions and inflammation.
Pathways
IL3RA interacts with the JAK/STAT pathway which is essential for signal transduction and transcription activation in response to IL-3 binding. This pathway involves proteins like Janus kinase 2 (JAK2) and Signal Transducer and Activator of Transcription 5 (STAT5) which transmit signals from IL3RA engagement to the cell nucleus promoting gene expression associated with cell proliferation and survival.
Specifications
Form
Lyophilized
Additional notes
Determined by SDS-PAGE and visualized by silver stain. Purified by native chromatography methods without the use of molecular tags.
General info
Function
Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 10527461). Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity).
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Post-translational modifications
Ubiquitinated by RNFT2 in response to IL3. Ubiquitination leads ligand-induced degradation by the proteasome. Ubiquitinated by RNF128 via 'Lys-27'-linked polyubiquitination, facilitating its degradation through the lysosomal pathway (PubMed:38702781).
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Molecular therapy. Methods & clinical development 18:571-581 PubMed32775492
2020
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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