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AB132543

Recombinant Human ILF3 protein

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(1 Publication)

Recombinant Human ILF3 protein is a Human Full Length protein, in the 1 to 702 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

DRBF, MPHOSPH4, NF90, ILF3, Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80

1 Images
SDS-PAGE - Recombinant Human ILF3 protein (AB132543)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ILF3 protein (AB132543)

12.5% SDS-PAGE Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

Q12906

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ILF3.
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Sequence info

[{"sequence":"MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVEAVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANKKKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEVPPPPNLRGRGRGGSIRGRGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSDFFTDCYGYHDFGSS","proteinLength":"Full Length","predictedMolecularWeight":"102.85 kDa","actualMolecularWeight":null,"aminoAcidEnd":702,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q12906","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ILF3 protein also known as Interleukin enhancer-binding factor 3 plays an important role in cellular mechanisms. It has a molecular mass of approximately 90 kDa and is expressed broadly in various tissues such as the liver kidney and spleen. ILF3 is part of the double-stranded RNA-binding proteins and it engages in regulating RNA metabolism processes. Scientists also refer to ILF3 as NF90 and it is known for its function in modulating gene expression by interacting with RNA.
Biological function summary

ILF3 interacts with RNA and proteins within the cell to control the stability and translation of mRNA. It acts as a component of various ribonucleoprotein complexes indicating its role in post-transcriptional regulation. ILF3 can bind to double-stranded RNA and single-stranded RNA motifs affecting the proliferation and differentiation of cells in which it is expressed. It also contributes significantly in cellular stress responses impacting how cells respond to environmental changes.

Pathways

ILF3 is involved in important cellular pathways such as RNA processing and antiviral defense mechanisms. In the RNA processing pathway ILF3 associates with proteins such as Drosha impacting microRNA biogenesis. Additionally in antiviral responses it identifies and binds viral RNAs initiating defensive cellular responses. These pathways position ILF3 as integral to maintaining cellular homeostasis during stress and infection.

ILF3 has connections to cancer and viral infections. Aberrations in ILF3 expression or function are observed in certain cancers where it influences tumor progression by interacting with oncogenic pathways. In viral infections like those caused by hepatitis C virus ILF3 contributes to host defense by modulating immune responses. It interacts with proteins like RIG-I in the context of viral infections facilitating antiviral signaling pathways to control disease progression.
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Specifications

Form

Liquid

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General info

Function

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed : 28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed : 21123651, PubMed : 34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed : 21123651, PubMed : 28625552).. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.

Post-translational modifications

Phosphorylated at Thr-188 and Thr-315 by PKR in response to certain RNA viruses. This phosphorylation results in the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3 where it can bind to viral RNAs and impede viral replication.. Methylated by protein arginine N-methyltransferase 1.. Ubiquitinated at Lys-297 in a TRIM47-dependent manner; this 'Lys-48'-linked ubiquitination promotes ILF3 degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed : 28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed : 21123651, PubMed : 34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed : 21123651, PubMed : 28625552).. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
See full target information ILF3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 69:1005-1016.e7 PubMed29547715

2018

Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Julia K Nussbacher,Gene W Yeo
View all publications
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