Recombinant Human ILT-3 protein (Tagged) is a Human Fragment protein, in the 17 to 257 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
P R T H M Q A G P L P K P T L W A E P G S V I S W G N S V T I W C Q G T L E A R E Y R L D K E E S P A P W D R Q N P L E P K N K A R F S I P S M T E D Y A G R Y R C Y Y R S P V G W S Q P S D P L E L V M T G A Y S K P T L S A L P S P L V T S G K S V T L L C Q S R S P M D T F L L I K E R A A H P L L H L R S E H G A Q Q H Q A E F P M S P V T S V H G G T Y R C F S S H G F S H Y L L S H P S D P L E L I V S G S L E D P R P S P T R S V S T A A G P E D Q P L M P T G S V P H S G L R R H W E V L I G V L V V S I L L L S L L L F L L L Q H W R Q G K H R T L A Q R Q A D F Q R P P G A A E P E P K D G G L Q R R S S P A A D V Q G E N F C A A V K N T Q P E D G V E M D T R Q S P H D E D P Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance (PubMed:11875462). Receptor for FN1 (PubMed:34089617). Receptor for apolipoprotein APOE (PubMed:30333625). Receptor for ALCAM/CD166 (PubMed:29263213). Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:9151699). Inhibits FCGR1A/CD64-mediated monocyte activation by inducing phosphatase-mediated down-regulation of the phosphorylation of multiple proteins including LCK, SYK, LAT and ERK, leading to a reduction in TNF production (PubMed:19833736). This inhibition of monocyte activation occurs at least in part via binding to FN1 (PubMed:34089617). Inhibits T cell proliferation, inducing anergy, suppressing the differentiation of IFNG-producing CD8+ cytoxic T cells and enhancing the generation of CD8+ T suppressor cells (PubMed:16493035, PubMed:19833736, PubMed:29263213). Induces up-regulation of CD86 on dendritic cells (PubMed:19860908). Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation (PubMed:11875462).
Alternative names
CD85k, ILT3, LIR5, LILRB4, Leukocyte immunoglobulin-like receptor subfamily B member 4, B4, CD85 antigen-like family member K, Immunoglobulin-like transcript 3, Leukocyte immunoglobulin-like receptor 5, Monocyte inhibitory receptor HM18, ILT-3, LIR-5
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Recombinant Human ILT-3 protein (Tagged) is a Human Fragment protein, in the 17 to 257 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8NHJ6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- P R T H M Q A G P L P K P T L W A E P G S V I S W G N S V T I W C Q G T L E A R E Y R L D K E E S P A P W D R Q N P L E P K N K A R F S I P S M T E D Y A G R Y R C Y Y R S P V G W S Q P S D P L E L V M T G A Y S K P T L S A L P S P L V T S G K S V T L L C Q S R S P M D T F L L I K E R A A H P L L H L R S E H G A Q Q H Q A E F P M S P V T S V H G G T Y R C F S S H G F S H Y L L S H P S D P L E L I V S G S L E D P R P S P T R S V S T A A G P E D Q P L M P T G S V P H S G L R R H W E V L I G V L V V S I L L L S L L L F L L L Q H W R Q G K H R T L A Q R Q A D F Q R P P G A A E P E P K D G G L Q R R S S P A A D V Q G E N F C A A V K N T Q P E D G V E M D T R Q S P H D E D P Q A
- Accession
- Q8NHJ6
- Protein length
- Fragment
- Predicted molecular weight
- 31 kDa
- Amino acids
- 17 to 257
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance (PubMed:11875462). Receptor for FN1 (PubMed:34089617). Receptor for apolipoprotein APOE (PubMed:30333625). Receptor for ALCAM/CD166 (PubMed:29263213). Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:9151699). Inhibits FCGR1A/CD64-mediated monocyte activation by inducing phosphatase-mediated down-regulation of the phosphorylation of multiple proteins including LCK, SYK, LAT and ERK, leading to a reduction in TNF production (PubMed:19833736). This inhibition of monocyte activation occurs at least in part via binding to FN1 (PubMed:34089617). Inhibits T cell proliferation, inducing anergy, suppressing the differentiation of IFNG-producing CD8+ cytoxic T cells and enhancing the generation of CD8+ T suppressor cells (PubMed:16493035, PubMed:19833736, PubMed:29263213). Induces up-regulation of CD86 on dendritic cells (PubMed:19860908). Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation (PubMed:11875462).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ILT-3 also known as Immunoglobulin-like transcript 3 is a protein predominantly expressed on dendritic cells and monocytes. It is a type I transmembrane protein belonging to the leukocyte immunoglobulin-like receptor family. The ILT-3 protein has a molecular mass of approximately 60 kDa. In addition to its presence on dendritic cells and monocytes ILT-3 can be detected in low levels on some types of macrophages. It plays a regulatory role in the immune system which can influence how the body responds to pathogens and tissue damage.
- Biological function summary
ILT-3 acts as an inhibitory receptor that modulates immune responses. It is often linked with the control of T cell activation and the maintenance of immune tolerance. ILT-3 does not form part of a larger protein complex. Its inhibitory function helps in preventing overactivation of the immune system by modulating the signaling pathways necessary for immune response. This modulation occurs through ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) domains that recruit phosphatases helping in dephosphorylating key signaling proteins and aiding in downregulation of immune cell activities.
- Pathways
The ILT-3 protein is involved in important immune regulatory pathways contributing significantly to the immune checkpoint pathway. It interacts with other immunoglobulin-like receptors and is known to influence pathways involving PD-1 (Programmed cell death protein 1) and CTLA-4 (Cytotoxic T-lymphocyte-associated protein 4). By affecting these pathways ILT-3 serves as a checkpoint that ensures immune homeostasis prevents excess inflammation and influences the balance between immune activation and suppression within the body.
- Associated diseases and disorders
ILT-3 has been implicated in autoimmune diseases and transplant rejection. It helps in suppressing immune responses that can lead to conditions like rheumatoid arthritis and could negatively affect organ transplant outcomes by minimizing the chance of rejection. ILT-3's role in these conditions connects it to proteins such as HLA-G and PD-L1 which are also involved in immune modulation and contribute to disease pathogenesis. Understanding ILT-3’s role provides insights into potential therapeutic targets for these diseases.
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1 product image
SDS-PAGE - Recombinant Human ILT-3 protein (Tagged) (ab271597)
SDS-PAGE analysis of 4 μg ab271597.
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