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AB271596

Recombinant Human ILT-3 protein (Tagged) (Biotin)

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Recombinant Human ILT-3 protein (Tagged) (Biotin) is a Human Fragment protein, in the 17 to 257 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE.

View Alternative Names

CD85k, ILT3, LIR5, LILRB4, Leukocyte immunoglobulin-like receptor subfamily B member 4, B4, CD85 antigen-like family member K, Immunoglobulin-like transcript 3, Leukocyte immunoglobulin-like receptor 5, Monocyte inhibitory receptor HM18, ILT-3, LIR-5

1 Images
SDS-PAGE - Recombinant Human ILT-3 protein (Tagged) (Biotin) (AB271596)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ILT-3 protein (Tagged) (Biotin) (AB271596)

SDS-PAGE analysis of 4 μg ab271596.

Key facts

Purity

>90% SDS-PAGE

Expression system

HEK 293 cells

Tags

Avi tag C-Terminus His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Conjugation

Biotin

Excitation/Emission
Accession

Q8NHJ6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This protein runs at a higher molecular weight due to glycosylation.</p>" } } }
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Product details

Enzymatically biotin-labeled using Avi-tag™ technology
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Sequence info

[{"sequence":"PRTHMQAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEDPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRHWEVLIGVLVVSILLLSLLLFLLLQHWRQGKHRTLAQRQADFQRPPGAAEPEPKDGGLQRRSSPAADVQGENFCAAVKNTQPEDGVEMDTRQSPHDEDPQA","proteinLength":"Fragment","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":257,"aminoAcidStart":17,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8NHJ6","tags":[{"tag":"Avi","terminus":"C-Terminus"},{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle|Store in the dark
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ILT-3 also known as Immunoglobulin-like transcript 3 is a protein predominantly expressed on dendritic cells and monocytes. It is a type I transmembrane protein belonging to the leukocyte immunoglobulin-like receptor family. The ILT-3 protein has a molecular mass of approximately 60 kDa. In addition to its presence on dendritic cells and monocytes ILT-3 can be detected in low levels on some types of macrophages. It plays a regulatory role in the immune system which can influence how the body responds to pathogens and tissue damage.
Biological function summary

ILT-3 acts as an inhibitory receptor that modulates immune responses. It is often linked with the control of T cell activation and the maintenance of immune tolerance. ILT-3 does not form part of a larger protein complex. Its inhibitory function helps in preventing overactivation of the immune system by modulating the signaling pathways necessary for immune response. This modulation occurs through ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) domains that recruit phosphatases helping in dephosphorylating key signaling proteins and aiding in downregulation of immune cell activities.

Pathways

The ILT-3 protein is involved in important immune regulatory pathways contributing significantly to the immune checkpoint pathway. It interacts with other immunoglobulin-like receptors and is known to influence pathways involving PD-1 (Programmed cell death protein 1) and CTLA-4 (Cytotoxic T-lymphocyte-associated protein 4). By affecting these pathways ILT-3 serves as a checkpoint that ensures immune homeostasis prevents excess inflammation and influences the balance between immune activation and suppression within the body.

ILT-3 has been implicated in autoimmune diseases and transplant rejection. It helps in suppressing immune responses that can lead to conditions like rheumatoid arthritis and could negatively affect organ transplant outcomes by minimizing the chance of rejection. ILT-3's role in these conditions connects it to proteins such as HLA-G and PD-L1 which are also involved in immune modulation and contribute to disease pathogenesis. Understanding ILT-3’s role provides insights into potential therapeutic targets for these diseases.
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Specifications

Form

Liquid

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General info

Function

Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance (PubMed : 11875462). Receptor for FN1 (PubMed : 34089617). Receptor for apolipoprotein APOE (PubMed : 30333625). Receptor for ALCAM/CD166 (PubMed : 29263213). Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed : 9151699). Inhibits FCGR1A/CD64-mediated monocyte activation by inducing phosphatase-mediated down-regulation of the phosphorylation of multiple proteins including LCK, SYK, LAT and ERK, leading to a reduction in TNF production (PubMed : 19833736). This inhibition of monocyte activation occurs at least in part via binding to FN1 (PubMed : 34089617). Inhibits T cell proliferation, inducing anergy, suppressing the differentiation of IFNG-producing CD8+ cytoxic T cells and enhancing the generation of CD8+ T suppressor cells (PubMed : 16493035, PubMed : 19833736, PubMed : 29263213). Induces up-regulation of CD86 on dendritic cells (PubMed : 19860908). Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation (PubMed : 11875462).

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Product protocols

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Target data

Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance (PubMed : 11875462). Receptor for FN1 (PubMed : 34089617). Receptor for apolipoprotein APOE (PubMed : 30333625). Receptor for ALCAM/CD166 (PubMed : 29263213). Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed : 9151699). Inhibits FCGR1A/CD64-mediated monocyte activation by inducing phosphatase-mediated down-regulation of the phosphorylation of multiple proteins including LCK, SYK, LAT and ERK, leading to a reduction in TNF production (PubMed : 19833736). This inhibition of monocyte activation occurs at least in part via binding to FN1 (PubMed : 34089617). Inhibits T cell proliferation, inducing anergy, suppressing the differentiation of IFNG-producing CD8+ cytoxic T cells and enhancing the generation of CD8+ T suppressor cells (PubMed : 16493035, PubMed : 19833736, PubMed : 29263213). Induces up-regulation of CD86 on dendritic cells (PubMed : 19860908). Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation (PubMed : 11875462).
See full target information LILRB4
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