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AB271595

Recombinant Human ILT-4 protein (Biotin) (Avi tag C-Terminus + His tag C-Terminus)

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Recombinant Human ILT-4 protein (Biotin) (Avi tag C-Terminus + His tag C-Terminus) is a Human Fragment protein, in the 22 to 458 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE.

View Alternative Names

CD85d, ILT4, LIR2, MIR10, LILRB2, Leukocyte immunoglobulin-like receptor subfamily B member 2, LIR-2, Leukocyte immunoglobulin-like receptor 2, CD85 antigen-like family member D, Immunoglobulin-like transcript 4, Monocyte/macrophage immunoglobulin-like receptor 10, ILT-4, MIR-10

1 Images
SDS-PAGE - Recombinant Human ILT-4 protein (Biotin) (Avi tag C-Terminus + His tag C-Terminus) (AB271595)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ILT-4 protein (Biotin) (Avi tag C-Terminus + His tag C-Terminus) (AB271595)

SDS-PAGE analysis of 4 μg ab271595.

Key facts

Purity

>90% SDS-PAGE

Expression system

HEK 293 cells

Tags

Avi tag C-Terminus His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Conjugation

Biotin

Excitation/Emission
Accession

Q8N423

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Proteins & Peptides

AB290106

Recombinant Human LILRB1 Protein

SDS-PAGE - Recombinant Human LILRB1 Protein (AB290106)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Assay Kits

AB269551

Human CD85d ELISA Kit (LILRB2)

Recombinant|SimpleStep

Sandwich ELISA - Human CD85d ELISA Kit (LILRB2) (AB269551)

  • 0
  • 0
Primary Antibodies

AB243107

Anti-ILT-4 antibody [EPR22081] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (AB243107)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This protein runs at a higher molecular weight due to glycosylation.</p>" } } }
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Product details

Enzymatically biotin-labeled using Avi-tag™ technology
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Sequence info

[{"sequence":"QTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEEEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRH","proteinLength":"Fragment","predictedMolecularWeight":"52 kDa","actualMolecularWeight":null,"aminoAcidEnd":458,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8N423","tags":[{"tag":"Avi","terminus":"C-Terminus"},{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle|Store in the dark
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ILT-4 also known as Immunoglobulin-like transcript 4 is a transmembrane protein with a molecular weight of approximately 45 kDa. The protein is part of the LILR family and is sometimes referred to as LILRB2 protein. ILT-4 is mainly expressed on myeloid lineage cells including monocytes and dendritic cells. The expression of ILT-4 is important for the modulation of the immune response. Biotinylated ILT-4 and biotinylated LILRB2 versions are used frequently in research to study their interactions and functions.
Biological function summary

ILT-4 plays an important role in the regulation of immune tolerance and inhibition. It is not part of a complex but interacts with other immune regulatory proteins. By binding to MHC class I molecules ILT-4 inhibits the activation of immune responses preventing over-activation and autoimmunity. The ILT-4 protein is critical for helping myeloid cells to maintain a balanced immune response often restricting unnecessary inflammation and promoting tolerance.

Pathways

ILT-4 influences immune signaling pathways such as those that control T cell receptor signaling. ILT-4 interactions with proteins like CD85d and other LILRA6 proteins showcase its involvement in negative regulatory signaling pathways. These pathways are essential for maintaining immune homeostasis and limiting immune-mediated damage during infections or tissue injury.

ILT-4 is implicated in cancer and autoimmune diseases such as multiple sclerosis. In cancers ILT-4 modulates the immune environment allowing tumors to evade immune surveillance. In the context of autoimmune disorders aberrant ILT-4 expression or function can lead to insufficient immune regulation contributing to disease progression. CD85d and LILRB2 proteins are also associated with these conditions further influencing ILT-4's role in disease mechanisms.
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Specifications

Form

Liquid

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General info

Function

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed : 11169396, PubMed : 12853576, PubMed : 16455647, PubMed : 20448110, PubMed : 27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed : 16455647, PubMed : 20448110, PubMed : 27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed : 11875462, PubMed : 12853576, PubMed : 9548455, PubMed : 9842885).

Post-translational modifications

Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.

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Product protocols

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Target data

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed : 11169396, PubMed : 12853576, PubMed : 16455647, PubMed : 20448110, PubMed : 27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed : 16455647, PubMed : 20448110, PubMed : 27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed : 11875462, PubMed : 12853576, PubMed : 9548455, PubMed : 9842885).
See full target information LILRB2
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