Recombinant Human ILT-4 protein (Tagged) (Biotin) is a Human Fragment protein, in the 22 to 458 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
Amino acid sequence
Q T G T I P K P T L W A E P D S V I T Q G S P V T L S C Q G S L E A Q E Y R L Y R E K K S A S W I T R I R P E L V K N G Q F H I P S I T W E H T G R Y G C Q Y Y S R A R W S E L S D P L V L V M T G A Y P K P T L S A Q P S P V V T S G G R V T L Q C E S Q V A F G G F I L C K E G E E E H P Q C L N S Q P H A R G S S R A I F S V G P V S P N R R W S H R C Y G Y D L N S P Y V W S S P S D L L E L L V P G V S K K P S L S V Q P G P V V A P G E S L T L Q C V S D V G Y D R F V L Y K E G E R D L R Q L P G R Q P Q A G L S Q A N F T L G P V S R S Y G G Q Y R C Y G A H N L S S E C S A P S D P L D I L I T G Q I R G T P F I S V Q P G P T V A S G E N V T L L C Q S W R Q F H T F L L T K A G A A D A P L R L R S I H E Y P K Y Q A E F P M S P V T S A H A G T Y R C Y G S L N S D P Y L L S H P S E P L E L V V S G P S M G S S P P P T G P I S T P A G P E D Q P L T P T G S D P Q S G L G R H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes This protein runs at a higher molecular weight due to glycosylation. |
Target data
Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885).
Alternative names
CD85d, ILT4, LIR2, MIR10, LILRB2, Leukocyte immunoglobulin-like receptor subfamily B member 2, LIR-2, Leukocyte immunoglobulin-like receptor 2, CD85 antigen-like family member D, Immunoglobulin-like transcript 4, Monocyte/macrophage immunoglobulin-like receptor 10, ILT-4, MIR-10
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Recombinant Human ILT-4 protein (Tagged) (Biotin) is a Human Fragment protein, in the 22 to 458 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
- Accession
- Q8N423-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- Q T G T I P K P T L W A E P D S V I T Q G S P V T L S C Q G S L E A Q E Y R L Y R E K K S A S W I T R I R P E L V K N G Q F H I P S I T W E H T G R Y G C Q Y Y S R A R W S E L S D P L V L V M T G A Y P K P T L S A Q P S P V V T S G G R V T L Q C E S Q V A F G G F I L C K E G E E E H P Q C L N S Q P H A R G S S R A I F S V G P V S P N R R W S H R C Y G Y D L N S P Y V W S S P S D L L E L L V P G V S K K P S L S V Q P G P V V A P G E S L T L Q C V S D V G Y D R F V L Y K E G E R D L R Q L P G R Q P Q A G L S Q A N F T L G P V S R S Y G G Q Y R C Y G A H N L S S E C S A P S D P L D I L I T G Q I R G T P F I S V Q P G P T V A S G E N V T L L C Q S W R Q F H T F L L T K A G A A D A P L R L R S I H E Y P K Y Q A E F P M S P V T S A H A G T Y R C Y G S L N S D P Y L L S H P S E P L E L V V S G P S M G S S P P P T G P I S T P A G P E D Q P L T P T G S D P Q S G L G R H
- Accession
- Q8N423
- Protein length
- Fragment
- Predicted molecular weight
- 52 kDa
- Amino acids
- 22 to 458
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, Fc tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885).
- Post-translational modifications
Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Enzymatically biotin-labeled using Avi-tag™ technology
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ILT-4 also known as Immunoglobulin-like transcript 4 is a transmembrane protein with a molecular weight of approximately 45 kDa. The protein is part of the LILR family and is sometimes referred to as LILRB2 protein. ILT-4 is mainly expressed on myeloid lineage cells including monocytes and dendritic cells. The expression of ILT-4 is important for the modulation of the immune response. Biotinylated ILT-4 and biotinylated LILRB2 versions are used frequently in research to study their interactions and functions.
- Biological function summary
ILT-4 plays an important role in the regulation of immune tolerance and inhibition. It is not part of a complex but interacts with other immune regulatory proteins. By binding to MHC class I molecules ILT-4 inhibits the activation of immune responses preventing over-activation and autoimmunity. The ILT-4 protein is critical for helping myeloid cells to maintain a balanced immune response often restricting unnecessary inflammation and promoting tolerance.
- Pathways
ILT-4 influences immune signaling pathways such as those that control T cell receptor signaling. ILT-4 interactions with proteins like CD85d and other LILRA6 proteins showcase its involvement in negative regulatory signaling pathways. These pathways are essential for maintaining immune homeostasis and limiting immune-mediated damage during infections or tissue injury.
- Associated diseases and disorders
ILT-4 is implicated in cancer and autoimmune diseases such as multiple sclerosis. In cancers ILT-4 modulates the immune environment allowing tumors to evade immune surveillance. In the context of autoimmune disorders aberrant ILT-4 expression or function can lead to insufficient immune regulation contributing to disease progression. CD85d and LILRB2 proteins are also associated with these conditions further influencing ILT-4's role in disease mechanisms.
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1 product image
SDS-PAGE - Recombinant Human ILT-4 protein (Tagged) (Biotin) (ab271595)
SDS-PAGE analysis of 4 μg ab271595.
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