Recombinant Human IMPA2 protein is a Human Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M K P S G E D Q A A L A A G P W E E C F Q A A V Q L A L R A G Q I I R K A L T E E K R V S T K T S A A D L V T E T D H L V E D L I I S E L R E R F P S H R F I A E E A A A S G A K C V L T H S P T W I I D P I D G T C N F V H R F P T V A V S I G F A V R Q E L E F G V I Y H C T E E R L Y T G R R G R G A F C N G Q R L R V S G E T D L S K A L V L T E I G P K R D P A T L K L F L S N M E R L L H A K A H G V R V I G S S T L A L C H L A S G A A D A Y Y Q F G L H C W D L A A A T V I I R E A G G I V I D T S G G P L D L M A C R V V A A S T R E M A M L I A Q A L Q T I N Y G R D D E K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Can use myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. Has been implicated as the pharmacological target for lithium Li(+) action in brain.
Alternative names
IMP.18P, IMPA2, Inositol monophosphatase 2, IMP 2, IMPase 2, Inositol-1(or 4)-monophosphatase 2, Myo-inositol monophosphatase A2
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Recombinant Human IMPA2 protein is a Human Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O14732-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M K P S G E D Q A A L A A G P W E E C F Q A A V Q L A L R A G Q I I R K A L T E E K R V S T K T S A A D L V T E T D H L V E D L I I S E L R E R F P S H R F I A E E A A A S G A K C V L T H S P T W I I D P I D G T C N F V H R F P T V A V S I G F A V R Q E L E F G V I Y H C T E E R L Y T G R R G R G A F C N G Q R L R V S G E T D L S K A L V L T E I G P K R D P A T L K L F L S N M E R L L H A K A H G V R V I G S S T L A L C H L A S G A A D A Y Y Q F G L H C W D L A A A T V I I R E A G G I V I D T S G G P L D L M A C R V V A A S T R E M A M L I A Q A L Q T I N Y G R D D E K
- Accession
- O14732
- Protein length
- Full Length
- Predicted molecular weight
- 33.5 kDa
- Amino acids
- 1 to 288
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Can use myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. Has been implicated as the pharmacological target for lithium Li(+) action in brain.
- Sequence similarities
Belongs to the inositol monophosphatase superfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IMPA2 also known as Inositol Monophosphatase 2 is an enzyme with a mass of approximately 29 kDa. This protein is expressed predominantly in the brain particularly in regions such as the cerebellum and cerebral cortex. IMPA2 catalyzes the dephosphorylation of inositol monophosphate to free inositol an essential step in the phosphatidylinositol signaling pathway. This enzyme plays a significant role in maintaining inositol and inositol-derived compounds important for cellular functions.
- Biological function summary
IMPA2 influences cellular processes by managing inositol phosphate recycling and inositol availability. This enzyme does not typically function as part of a larger protein complex. Its activity helps control the levels of inositol in cells impacting signaling pathways that rely on phosphatidylinositol and its derivatives. Through this regulation IMPA2 supports functions like cell growth and survival indirectly affecting neurotransmission and hormone signaling.
- Pathways
IMPA2 assumes a central role in the phosphatidylinositol signaling pathway which influences a variety of cellular responses. It interacts closely with the phosphoinositide pathway sharing a relationship with proteins like G-protein coupled receptors and protein kinase C. The enzyme's activity ensures the continuous functioning of these pathways by supplying inositol an important molecule that these proteins need for activation and signaling transmission.
- Associated diseases and disorders
Researchers have linked IMPA2 to bipolar disorder and schizophrenia. Reduced or altered activity of IMPA2 might contribute to imbalance in inositol signaling which is a hypothesis in the pathophysiology of these psychiatric conditions. The protein shows connections to other molecules involved in these disorders such as glycogen synthase kinase-3 (GSK3) which plays a role in mood regulation and neuroplasticity. Understanding the role of IMPA2 in these diseases could offer insights into potential therapeutic approaches.
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1 product image
SDS-PAGE - Recombinant Human IMPA2 protein (ab104020)
15% SDS-PAGE analysis of ab104020 (3 μg).
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