Recombinant Human IMPDH1 protein is a Human Full Length protein, in the 1 to 514 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
< i > M G S S H H H H H H S S G L V P R G S H < / i > M A D Y L I S G G T G Y V P E D G L T A Q Q L F A S A D G L T Y N D F L I L P G F I D F I A D E V D L T S A L T R K I T L K T P L I S S P M D T V T E A D M A I A M A L M G G I G F I H H N C T P E F Q A N E V R K V K K F E Q G F I T D P V V L S P S H T V G D V L E A K M R H G F S G I P I T E T G T M G S K L V G I V T S R D I D F L A E K D H T T L L S E V M T P R I E L V V A P A G V T L K E A N E I L Q R S K K G K L P I V N D C D E L V A I I A R T D L K K N R D Y P L A S K D S Q K Q L L C G A A V G T R E D D K Y R L D L L T Q A G V D V I V L D S S Q G N S V Y Q I A M V H Y I K Q K Y P H L Q V I G G N V V T A A Q A K N L I D A G V D G L R V G M G C G S I C I T Q E V M A C G R P Q G T A V Y K V A E Y A R R F G V P I I A D G G I Q T V G H V V K A L A L G A S T V M M G S L L A A T T E A P G E Y F F S D G V R L K K Y R G M G S L D A M E K S S S S Q K R Y F S E G D K V K I A Q G V S G S I Q D K G S I Q K F V P Y L I A G I Q H G C Q D I G A R S L S V L R S M M Y S G E L K F E K R T M S A Q I E G G V H G L H S Y E K R L Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
Alternative names
IMPD1, IMPDH1, Inosine-5'-monophosphate dehydrogenase 1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPDH-I
Recommended products
Recombinant Human IMPDH1 protein is a Human Full Length protein, in the 1 to 514 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P20839-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- < i > M G S S H H H H H H S S G L V P R G S H < / i > M A D Y L I S G G T G Y V P E D G L T A Q Q L F A S A D G L T Y N D F L I L P G F I D F I A D E V D L T S A L T R K I T L K T P L I S S P M D T V T E A D M A I A M A L M G G I G F I H H N C T P E F Q A N E V R K V K K F E Q G F I T D P V V L S P S H T V G D V L E A K M R H G F S G I P I T E T G T M G S K L V G I V T S R D I D F L A E K D H T T L L S E V M T P R I E L V V A P A G V T L K E A N E I L Q R S K K G K L P I V N D C D E L V A I I A R T D L K K N R D Y P L A S K D S Q K Q L L C G A A V G T R E D D K Y R L D L L T Q A G V D V I V L D S S Q G N S V Y Q I A M V H Y I K Q K Y P H L Q V I G G N V V T A A Q A K N L I D A G V D G L R V G M G C G S I C I T Q E V M A C G R P Q G T A V Y K V A E Y A R R F G V P I I A D G G I Q T V G H V V K A L A L G A S T V M M G S L L A A T T E A P G E Y F F S D G V R L K K Y R G M G S L D A M E K S S S S Q K R Y F S E G D K V K I A Q G V S G S I Q D K G S I Q K F V P Y L I A G I Q H G C Q D I G A R S L S V L R S M M Y S G E L K F E K R T M S A Q I E G G V H G L H S Y E K R L Y
- Accession
- P20839
- Protein length
- Full Length
- Predicted molecular weight
- 57.5 kDa
- Amino acids
- 1 to 514
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab97410 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
- Sequence similarities
Belongs to the IMPDH/GMPR family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IMPDH1 also known as Inosine-5'-monophosphate dehydrogenase 1 plays an important role in purine metabolism by catalyzing the conversion of inosine-5'-monophosphate (IMP) to xanthosine-5'-monophosphate (XMP). The protein's mass is approximately 55 kDa. IMPDH1 can be found in various tissues but shows a higher expression level in the retina. This enzyme's activity is essential for the regulation of cellular proliferation and differentiation due to its influence on guanine nucleotide synthesis.
- Biological function summary
IMPDH1 contributes significantly to the cellular levels of guanine nucleotides by being part of the purine nucleotide biosynthesis pathway. IMPDH1 does not typically form a larger protein complex but its homotetrameric structure is important for its enzymatic function. It plays a role in maintaining proper nucleotide balance within cells which is important for DNA and RNA synthesis and consequently cell division.
- Pathways
Purine metabolism involves IMPDH1 and emphasizes its importance in the guanine nucleotide biosynthesis pathway and salvage pathway. This protein functions alongside IMPDH2 another isoform which shares a similar enzymatic role but differs in expression patterns across tissues. Together these enzymes help regulate the de novo synthesis pathway finely tuning the availability of GDP and GTP necessary for cellular functions.
- Associated diseases and disorders
IMPDH1 mutations have links to retinitis pigmentosa a degenerative eye disease leading to vision loss. This association implicates also other proteins involved in retinal function such as rhodopsin. Additionally IMPDH1 is implicated in Lesch-Nyhan syndrome a rare disorder impacting purine metabolism. There is a connection between IMPDH1 activity and the hypoxanthine-guanine phosphoribosyltransferase enzyme which when deficient gives rise to this syndrome.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human IMPDH1 protein (ab97410)
15% SDS-PAGE showing ab97410 at approximately 57.5kDa (3μg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com