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AB97410

Recombinant Human IMPDH1 protein (His tag N-Terminus)

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Recombinant Human IMPDH1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 514 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

IMPD1, IMPDH1, Inosine-5'-monophosphate dehydrogenase 1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPDH-I

1 Images
SDS-PAGE - Recombinant Human IMPDH1 protein (His tag N-Terminus) (AB97410)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human IMPDH1 protein (His tag N-Terminus) (AB97410)

15% SDS-PAGE showing ab97410 at approximately 57.5kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P20839

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Proteins & Peptides

AB113608

Recombinant Human IMPDH2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human IMPDH2 protein (His tag N-Terminus) (AB113608)

  • 0
  • 0
Primary Antibodies

AB140611

Anti-HSPC150/UBE2T antibody [EPR9446]

RabMAb|Recombinant

Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (AB140611)

  • 4
  • 1
Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
  • 90
Primary Antibodies

AB183340

Anti-Nucleophosmin antibody [SP236]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] (AB183340)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMADYLISGGTGYVPEDGLTAQQLFASADGLTYNDFLILPGFIDFIADEVDLTSALTRKITLKTPLISSPMDTVTEADMAIAMALMGGIGFIHHNCTPEFQANEVRKVKKFEQGFITDPVVLSPSHTVGDVLEAKMRHGFSGIPITETGTMGSKLVGIVTSRDIDFLAEKDHTTLLSEVMTPRIELVVAPAGVTLKEANEILQRSKKGKLPIVNDCDELVAIIARTDLKKNRDYPLASKDSQKQLLCGAAVGTREDDKYRLDLLTQAGVDVIVLDSSQGNSVYQIAMVHYIKQKYPHLQVIGGNVVTAAQAKNLIDAGVDGLRVGMGCGSICITQEVMACGRPQGTAVYKVAEYARRFGVPIIADGGIQTVGHVVKALALGASTVMMGSLLAATTEAPGEYFFSDGVRLKKYRGMGSLDAMEKSSSSQKRYFSEGDKVKIAQGVSGSIQDKGSIQKFVPYLIAGIQHGCQDIGARSLSVLRSMMYSGELKFEKRTMSAQIEGGVHGLHSYEKRLY","proteinLength":"Full Length","predictedMolecularWeight":"57.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":514,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P20839","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IMPDH1 also known as Inosine-5'-monophosphate dehydrogenase 1 plays an important role in purine metabolism by catalyzing the conversion of inosine-5'-monophosphate (IMP) to xanthosine-5'-monophosphate (XMP). The protein's mass is approximately 55 kDa. IMPDH1 can be found in various tissues but shows a higher expression level in the retina. This enzyme's activity is essential for the regulation of cellular proliferation and differentiation due to its influence on guanine nucleotide synthesis.
Biological function summary

IMPDH1 contributes significantly to the cellular levels of guanine nucleotides by being part of the purine nucleotide biosynthesis pathway. IMPDH1 does not typically form a larger protein complex but its homotetrameric structure is important for its enzymatic function. It plays a role in maintaining proper nucleotide balance within cells which is important for DNA and RNA synthesis and consequently cell division.

Pathways

Purine metabolism involves IMPDH1 and emphasizes its importance in the guanine nucleotide biosynthesis pathway and salvage pathway. This protein functions alongside IMPDH2 another isoform which shares a similar enzymatic role but differs in expression patterns across tissues. Together these enzymes help regulate the de novo synthesis pathway finely tuning the availability of GDP and GTP necessary for cellular functions.

IMPDH1 mutations have links to retinitis pigmentosa a degenerative eye disease leading to vision loss. This association implicates also other proteins involved in retinal function such as rhodopsin. Additionally IMPDH1 is implicated in Lesch-Nyhan syndrome a rare disorder impacting purine metabolism. There is a connection between IMPDH1 activity and the hypoxanthine-guanine phosphoribosyltransferase enzyme which when deficient gives rise to this syndrome.
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Specifications

Form

Liquid

Additional notes

ab97410 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

Sequence similarities

Belongs to the IMPDH/GMPR family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
See full target information IMPDH1
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