Recombinant Human IMPDH2 protein is a Human Full Length protein, in the 1 to 514 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A D Y L I S G G T S Y V P D D G L T A Q Q L F N C G D G L T Y N D F L I L P G Y I D F T A D Q V D L T S A L T K K I T L K T P L V S S P M D T V T E A G M A I A M A L T G G I G F I H H N C T P E F Q A N E V R K V K K Y E Q G F I T D P V V L S P K D R V R D V F E A K A R H G F C G I P I T D T G R M G S R L V G I I S S R D I D F L K E E E H D C F L E E I M T K R E D L V V A P A G I T L K E A N E I L Q R S K K G K L P I V N E D D E L V A I I A R T D L K K N R D Y P L A S K D A K K Q L L C G A A I G T H E D D K Y R L D L L A Q A G V D V V V L D S S Q G N S I F Q I N M I K Y I K D K Y P N L Q V I G G N V V T A A Q A K N L I D A G V D A L R V G M G S G S I C I T Q E V L A C G R P Q A T A V Y K V S E Y A R R F G V P V I A D G G I Q N V G H I A K A L A L G A S T V M M G S L L A A T T E A P G E Y F F S D G I R L K K Y R G M G S L D A M D K H L S S Q N R Y F S E A D K I K V A Q G V S G A V Q D K G S I H K F V P Y L I A G I Q H S C Q D I G A K S L T Q V R A M M Y S G E L K F E K R T S S A Q V E G G V H S L H S Y E K R L F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (PubMed:7763314, PubMed:7903306). Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (PubMed:14766016). It may also have a role in the development of malignancy and the growth progression of some tumors.
Alternative names
IMPD2, IMPDH2, Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, Inosine-5'-monophosphate dehydrogenase type II, IMP dehydrogenase II, IMPDH-II
Recommended products
Recombinant Human IMPDH2 protein is a Human Full Length protein, in the 1 to 514 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P12268-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A D Y L I S G G T S Y V P D D G L T A Q Q L F N C G D G L T Y N D F L I L P G Y I D F T A D Q V D L T S A L T K K I T L K T P L V S S P M D T V T E A G M A I A M A L T G G I G F I H H N C T P E F Q A N E V R K V K K Y E Q G F I T D P V V L S P K D R V R D V F E A K A R H G F C G I P I T D T G R M G S R L V G I I S S R D I D F L K E E E H D C F L E E I M T K R E D L V V A P A G I T L K E A N E I L Q R S K K G K L P I V N E D D E L V A I I A R T D L K K N R D Y P L A S K D A K K Q L L C G A A I G T H E D D K Y R L D L L A Q A G V D V V V L D S S Q G N S I F Q I N M I K Y I K D K Y P N L Q V I G G N V V T A A Q A K N L I D A G V D A L R V G M G S G S I C I T Q E V L A C G R P Q A T A V Y K V S E Y A R R F G V P V I A D G G I Q N V G H I A K A L A L G A S T V M M G S L L A A T T E A P G E Y F F S D G I R L K K Y R G M G S L D A M D K H L S S Q N R Y F S E A D K I K V A Q G V S G A V Q D K G S I H K F V P Y L I A G I Q H S C Q D I G A K S L T Q V R A M M Y S G E L K F E K R T S S A Q V E G G V H S L H S Y E K R L F
- Accession
- P12268
- Protein length
- Full Length
- Predicted molecular weight
- 58 kDa
- Amino acids
- 1 to 514
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113608 was purified using conventional chromatography.
General info
- Function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (PubMed:7763314, PubMed:7903306). Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (PubMed:14766016). It may also have a role in the development of malignancy and the growth progression of some tumors.
- Sequence similarities
Belongs to the IMPDH/GMPR family.
- Post-translational modifications
Ubiquitinated leading to its degradation by the proteasome.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IMPDH2 also known as inosine monophosphate dehydrogenase 2 plays an important role in the de novo synthesis of guanine nucleotides. This enzyme with a molecular mass of approximately 56 kDa catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) an important step in the nucleotide biosynthesis. IMPDH2 is expressed in various tissues across the body with high expression in proliferating cells due to increased nucleotide requirements.
- Biological function summary
IMPDH2 functions as a homotetramer forming part of a larger complex that regulates nucleotide levels. This regulation is critical for cell growth differentiation and proliferation. IMPDH2 activity drives the synthesis of guanine nucleotides which are essential for DNA and RNA synthesis influencing cellular communication and energy transfer.
- Pathways
The target IMPDH2 has a significant role in the purine nucleotide biosynthesis pathway aligning with other enzymes like GMP synthetase. This pathway is critical in maintaining cellular nucleotide pools. IMPDH2’s activity closely interacts with guanosine monophosphate reductase within the same pathway ensuring the balance between different types of nucleotides necessary for various cellular processes.
- Associated diseases and disorders
Aberrations in IMPDH2 activity link to immunological diseases and cancer. Overexpression or upregulation of this enzyme contributes to the rapid proliferation seen in cancerous cells by maintaining high guanine nucleotide levels. Additionally IMPDH2 serves as a target for immunosuppressive drugs used in autoimmune conditions highlighting its relationship with T- and B-cell proliferation. This positions IMPDH2 in therapeutic strategies alongside its pathway partners to modulate disease outcomes.
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SDS-PAGE - Recombinant Human IMPDH2 protein (ab113608)
15% SDS-PAGE analysis of ab113608 (3μg)
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