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AB127382

Recombinant Human Importin4/Imp4 protein

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Recombinant Human Importin4/Imp4 protein is a Human Fragment protein, in the 136 to 289 aa range, expressed in Escherichia coli, with 80%, suitable for SDS-PAGE.

View Alternative Names

IMP4B, RANBP4, IPO4, Importin-4, Imp4, Importin-4b, Ran-binding protein 4, Imp4b, RanBP4

Key facts

Purity

80%

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8TEX9

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Importin4
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"17.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":289,"aminoAcidStart":136,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8TEX9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Importin4 (Imp4) also known as Ipo4 is a nuclear import receptor with an approximate mass of 130 kDa. This protein facilitates the transport of molecules such as ribosomal proteins and histones into the nucleus. Expression of Importin4 occurs in various tissues including the brain testis and liver. Importin4 plays a significant mechanical role by recognizing nuclear localization signals on cargo molecules and mediating their translocation across the nuclear envelope.
Biological function summary

Importin4 functions as part of the nuclear transport system a complex network essential for cellular operations. Importin4 interacts with the nuclear pore complex to enable import of specific protein cargo into the nucleus. By partnering with karyopherins and Ran-GTPase Importin4 participates in the active transport mechanism ensuring the correct localization of proteins that are critical to genomic regulation processes.

Pathways

Importin4 plays a role in the nuclear transport pathway and is associated with the ribosome biogenesis pathway. This protein works alongside proteins like Ran-GTPase enhancing the translocation of ribosomal components pivotal for cellular growth and protein synthesis. Importin4 also aids in the shuttling of histones which are essential to chromatin assembly and DNA transcription regulation.

Importin4 has connections to cancer and neurodegenerative diseases. Altered expression and mutations in Importin4 may disrupt cellular homeostasis and nuclear transport linking it to tumorigenesis. Additionally associations with proteins such as karyopherin alpha importers indicate its involvement in pathological states affecting the nervous system potentially contributing to conditions such as Alzheimer's disease. Understanding Importin4's interactions could provide insights into these complex diseases.
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Specifications

Form

Lyophilized

Additional notes

Purity is 80% by SDS-PAGE.

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General info

Function

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR (PubMed : 11823430, PubMed : 16207705, PubMed : 17682055, PubMed : 21454524). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed : 11823430, PubMed : 16207705). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 11823430, PubMed : 16207705). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed : 11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 11823430). Mediates the nuclear import of the histone H3-H4 dimer when in complex with ASF1 (ASF1A or ASF1B) (PubMed : 21454524, PubMed : 29408485). Mediates the ligand-independent nuclear import of vitamin D receptor (VDR) (PubMed : 16207705). In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS (PubMed : 12610148).

Sequence similarities

Belongs to the importin beta family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR (PubMed : 11823430, PubMed : 16207705, PubMed : 17682055, PubMed : 21454524). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed : 11823430, PubMed : 16207705). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 11823430, PubMed : 16207705). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed : 11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 11823430). Mediates the nuclear import of the histone H3-H4 dimer when in complex with ASF1 (ASF1A or ASF1B) (PubMed : 21454524, PubMed : 29408485). Mediates the ligand-independent nuclear import of vitamin D receptor (VDR) (PubMed : 16207705). In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS (PubMed : 12610148).
See full target information IPO4
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