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AB135223

Recombinant Human INCENP protein (His tag N-Terminus)

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Recombinant Human INCENP protein (His tag N-Terminus) is a Human Fragment protein, in the 783 to 918 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.
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SDS-PAGE - Recombinant Human INCENP protein (His tag N-Terminus) (AB135223)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human INCENP protein (His tag N-Terminus) (AB135223)

SDS-PAGE analysis of ab135223.

Key facts

Purity

>90% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

No

Accession

Q9NQS7-1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

  • 0
  • 0
Primary Antibodies

AB12183

Anti-INCENP antibody

  • 4
  • 6
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Proteins & Peptides

AB93731

Recombinant human PPIL2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human PPIL2 protein (His tag N-Terminus) (AB93731)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab135223 can be used for Aurora B and Aurora C activation<i> in vitro</i>.</p>" } } }
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Sequence info

[{"linker":null,"sequence":"AAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH","proteinLength":"Fragment","predictedMolecularWeight":"19 kDa","actualMolecularWeight":null,"aminoAcidEnd":918,"aminoAcidStart":783,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9NQS7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INCENP short for Inner Centromere Protein plays a mechanical role during cell division. It acts as an important component of the chromosomal passenger complex (CPC) an important player in mitosis. INCENP is approximately 95 kDa in mass and is expressed largely in dividing cells. It helps to regulate chromosome alignment segregation and cytokinesis by interacting with other CPC members. Its activity is centered on the centromeres and the spindle midzone making it essential for proper mitotic progression.
Biological function summary

INCENP functions within the CPC which includes the kinase Aurora B Survivin and Borealin. This complex facilitates proper chromosomal alignment and segregation during mitosis. INCENP links Aurora B kinase activity to the centromere essential for correct chromosome bi-orientation and tension sensing. As a result INCENP stabilizes the architecture of the mitotic spindle ensuring successful cell cycle completion. This process is vital for maintaining genomic stability in proliferating cells.

Pathways

INCENP participates in the signaling cascades that control mitosis and cellular division. It is involved in the spindle assembly checkpoint pathway ensuring that cells do not progress through mitosis with misaligned chromosomes. INCENP interacts closely with proteins such as Aurora B and Survivin within this checkpoint mechanism. These interactions ensure that the cell cycle does not proceed until all chromosomes are correctly attached to the spindle apparatus thereby safeguarding against aneuploidy.

INCENP has strong connections to cancer and various chromosomal instability syndromes. Misregulation or mutation of INCENP can lead to an improper chromosomal segregation resulting in aneuploidy a hallmark of many cancer types. Its association with Aurora B and the other CPC components further implicates its role in tumorigenesis. Alterations in INCENP function could also contribute to disorders characterized by genomic instability highlighting its importance in cell division and genomic integrity.
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General info

Function

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed : 12925766, PubMed : 15316025, PubMed : 27332895). The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC (PubMed : 27332895). Required for localization of CBX5 to mitotic centromeres (PubMed : 21346195). Controls the kinetochore localization of BUB1 (PubMed : 16760428).

Sequence similarities

Belongs to the INCENP family.

Post-translational modifications

Phosphorylation by AURKB or AURKC at its C-terminal part is important for AURKB or AURKC activation by INCENP.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed : 12925766, PubMed : 15316025, PubMed : 27332895). The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC (PubMed : 27332895). Required for localization of CBX5 to mitotic centromeres (PubMed : 21346195). Controls the kinetochore localization of BUB1 (PubMed : 16760428).
See full target information INCENP

Alternative Names

Inner centromere protein, INCENP
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