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AB135010

Recombinant Human iNOS protein (His-DDDDK tag N-Terminus)

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Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) is a Human Full Length protein, in the 2 to 1153 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE.

View Alternative Names

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

2 Images
Western blot - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (AB135010)
  • WB

Supplier Data

Western blot - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (AB135010)

Blocking/Dilution buffer : 5% NFDM/TBST. Exposure time : Lane 1 : 1 second; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (<a href='/en-us/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human eNOS protein (His-DDDDK tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-enos-protein-tagged-ab198066'>ab198066</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (ab135010) at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

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SDS-PAGE - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (AB135010)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (AB135010)

10% SDS-PAGE analysis of ab135010 (1 μg), followed by Coomassie staining.
Lane 1 : ab135010
Lane 2 : MW marker

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His-DDDDK tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P35228

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.65% Sodium chloride, 0.64% Tris HCl, 0.06% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.017% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB15323

Anti-iNOS antibody

BOND RX™ Validated

Western blot - Anti-iNOS antibody (AB15323)

  • 4
  • 20
Primary Antibodies

AB233706

Anti-IL-6 antibody [EPR21711]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-IL-6 antibody [EPR21711] (AB233706)

  • 5
  • 1
Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

  • 5
  • 1
Assay Kits

AB211084

Nitric Oxide Synthase Activity Assay Kit (Fluorometric)

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"133 kDa","actualMolecularWeight":null,"aminoAcidEnd":1153,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P35228","tags":[{"tag":"His-DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).

Sequence similarities

Belongs to the NOS family.

Post-translational modifications

Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.

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Product protocols

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).
See full target information NOS2
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