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Recombinant Human Insig2 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
Alternative names=Insulin-induced gene 2 protein, INSIG-2, INSIG2
- Purity
>=85% SDS-PAGE
- Expression system
Cell free
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M A E G E T E S P G P K K C G P Y I S S V T S Q S V N L M I R G V V L F F I G V F L A L V L N L L Q I Q R N V T L F P P D V I A S I F S S A W W V P P C C G T A S A V I G L L Y P C I D R H L G E P H K F K R E W S S V M R C V A V F V G I N H A S A K V D F D N N I Q L S L T L A A L S I G L W W T F D R S R S G F G L G V G I A F L A T V V T Q L L V Y N G V Y Q Y T S P D F L Y V R S W L P C I F F A G G I T M G N I G R Q L A M Y E C K V I A E K S H Q E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human Insig2 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
Alternative names=Insulin-induced gene 2 protein, INSIG-2, INSIG2
Key facts
- Purity
>=85% SDS-PAGE
- Expression system
Cell free
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Predicted molecular weight
32.2 kDa
- Amino acids
1 to 225
- Sequence
M A E G E T E S P G P K K C G P Y I S S V T S Q S V N L M I R G V V L F F I G V F L A L V L N L L Q I Q R N V T L F P P D V I A S I F S S A W W V P P C C G T A S A V I G L L Y P C I D R H L G E P H K F K R E W S S V M R C V A V F V G I N H A S A K V D F D N N I Q L S L T L A A L S I G L W W T F D R S R S G F G L G V G I A F L A T V V T Q L L V Y N G V Y Q Y T S P D F L Y V R S W L P C I F F A G G I T M G N I G R Q L A M Y E C K V I A E K S H Q E
- Accession
- Q9Y5U4-1
- Protein length
Full Length
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol
Specifications
- Form
Lyophilized
General info
- Function
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR (PubMed:12242332, PubMed:16606821, PubMed:32322062). Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Binds oxysterol, including 22-hydroxycholesterol, 24-hydroxycholesterol, 25-hydroxycholesterol and 27-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum (PubMed:26160948, PubMed:17428920, PubMed:32322062). In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi (PubMed:32322062). Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG2 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase RNF139 (PubMed:16606821, PubMed:22143767).
- Sequence similarities
Belongs to the INSIG family.
- Post-translational modifications
Phosphorylation at Ser-151 by PCK1 reduces binding to oxysterol, disrupting the interaction between INSIG2 and SCAP, thereby promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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