Recombinant Human Insig2 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A E G E T E S P G P K K C G P Y I S S V T S Q S V N L M I R G V V L F F I G V F L A L V L N L L Q I Q R N V T L F P P D V I A S I F S S A W W V P P C C G T A S A V I G L L Y P C I D R H L G E P H K F K R E W S S V M R C V A V F V G I N H A S A K V D F D N N I Q L S L T L A A L S I G L W W T F D R S R S G F G L G V G I A F L A T V V T Q L L V Y N G V Y Q Y T S P D F L Y V R S W L P C I F F A G G I T M G N I G R Q L A M Y E C K V I A E K S H Q E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR (PubMed:12242332, PubMed:16606821, PubMed:32322062). Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Binds oxysterol, including 22-hydroxycholesterol, 24-hydroxycholesterol, 25-hydroxycholesterol and 27-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum (PubMed:17428920, PubMed:26160948, PubMed:32322062). In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi (PubMed:32322062). Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG2 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase RNF139 (PubMed:16606821, PubMed:22143767).
Alternative names
Insulin-induced gene 2 protein, INSIG-2, INSIG2
Recommended products
Recombinant Human Insig2 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9Y5U4-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol
Sequence info
Amino acid sequence
- M A E G E T E S P G P K K C G P Y I S S V T S Q S V N L M I R G V V L F F I G V F L A L V L N L L Q I Q R N V T L F P P D V I A S I F S S A W W V P P C C G T A S A V I G L L Y P C I D R H L G E P H K F K R E W S S V M R C V A V F V G I N H A S A K V D F D N N I Q L S L T L A A L S I G L W W T F D R S R S G F G L G V G I A F L A T V V T Q L L V Y N G V Y Q Y T S P D F L Y V R S W L P C I F F A G G I T M G N I G R Q L A M Y E C K V I A E K S H Q E
- Accession
- Q9Y5U4
- Protein length
- Full Length
- Predicted molecular weight
- 32.2 kDa
- Amino acids
- 1 to 225
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR (PubMed:12242332, PubMed:16606821, PubMed:32322062). Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Binds oxysterol, including 22-hydroxycholesterol, 24-hydroxycholesterol, 25-hydroxycholesterol and 27-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum (PubMed:17428920, PubMed:26160948, PubMed:32322062). In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi (PubMed:32322062). Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG2 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase RNF139 (PubMed:16606821, PubMed:22143767).
- Sequence similarities
Belongs to the INSIG family.
- Post-translational modifications
Phosphorylation at Ser-151 by PCK1 reduces binding to oxysterol, disrupting the interaction between INSIG2 and SCAP, thereby promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Insig2 short for Insulin Induced Gene 2 is a protein that plays an important role in lipid metabolism regulation. It has a mass of approximately 29 kDa and is also known as INSIG-2 and SREBP-inhibiting protein 2. This protein is embedded in the endoplasmic reticulum membrane and its expression occurs in tissues such as the liver adipose tissue and the brain. Insig2 functions as a regulatory protein binding to other proteins and controlling their activities related to cholesterol and fatty acid synthesis.
- Biological function summary
The Insig2 protein serves as an integral component in the regulation of lipid homeostasis. It participates in a protein complex including sterol regulatory element-binding proteins (SREBPs) and SCAP acting as an anchor to retain the SREBP-SCAP complex in the endoplasmic reticulum. By stopping the complex from being transported to the Golgi Insig2 prevents the activation of SREBPs which reduces cholesterol synthesis in cells. Insig2's role in maintaining lipid levels is significant in balancing energy metabolism.
- Pathways
Insig2 is directly involved in the cholesterol biosynthesis pathway and the fatty acid metabolism pathway. In these pathways Insig2 works alongside proteins such as SCAP and SREBPs halting SREBPs' transport and activation when cellular sterol levels are high. This regulation plays a critical role in ensuring lipid levels remain in balance emphasizing Insig2's importance in the response to sterol changes within the cell.
- Associated diseases and disorders
Insig2 shows an association with metabolic disorders like obesity and type 2 diabetes. Variations in the Insig2 gene may affect the regulation of lipid metabolism contributing to these conditions. Insig2 also interacts with SREBPs in this context as any disturbance in the Insig2-SREBPs regulatory mechanism can disturb normal lipid processing leading to metabolic dysregulation associated with these diseases.
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SDS-PAGE - Recombinant Human Insig2 protein (ab289767)
SDS-PAGE analysis of ab289767
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