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AB277027

Recombinant Human Integrin alpha 5 + beta 1 protein (Tagged)

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Recombinant Human Integrin alpha 5 + beta 1 protein (Tagged) is a Human Fragment protein, in the 1 to 728 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

CD49e, FNRA, ITGA5, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

1 Images
SDS-PAGE - Recombinant Human Integrin alpha 5 + beta 1 protein (Tagged) (AB277027)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Integrin alpha 5 + beta 1 protein (Tagged) (AB277027)

SDS-PAGE analysis of ab277027

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P08648

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Proteins & Peptides

AB287921

Recombinant human Integrin alpha V + beta 3 protein

SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (AB287921)

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Primary Antibodies

AB179475

Anti-Integrin alpha V antibody [EPR16800]

KO Validated|RabMAb|Recombinant

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

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Proteins & Peptides

AB114240

Recombinant Human Integrin alpha V protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Integrin alpha V protein (GST tag N-Terminus) (AB114240)

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Primary Antibodies

AB150361

Anti-Integrin alpha 5 antibody [EPR7854]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (AB150361)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD","proteinLength":"Fragment","predictedMolecularWeight":"83 kDa","actualMolecularWeight":null,"aminoAcidEnd":728,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P05556","tags":[{"tag":"His","terminus":"C-Terminus"}]},{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"109 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P08648","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 5 beta 1 also known as alpha 5 beta 1 integrin is a cell surface receptor that facilitates cell adhesion and signaling. This integrin is a heterodimer composed of alpha 5 and beta 1 subunits each contributing to its structure and function. The molecular mass of integrin alpha 5 beta 1 is approximately 170 kDa. It predominantly expresses on the surface of fibroblasts endothelial cells and some epithelial cells where it plays an important role in mediating interactions between the cell and the extracellular matrix (ECM).
Biological function summary

Integrin alpha 5 beta 1 regulates processes like cell adhesion migration and differentiation. It binds to fibronectin in the ECM forming part of the adhesion complexes needed for cellular attachment and survival. This integrin couples with the actin cytoskeleton through talin and vinculin initiating signaling cascades that adjust cellular responses to changes in the microenvironment. Its mechanical linkage to the ECM helps sustain structural integrity affecting tissue homeostasis.

Pathways

Integrin alpha 5 beta 1 participates in the focal adhesion and the MAPK signaling pathway each with significant influence on cell behavior. In focal adhesion it partners with focal adhesion kinase (FAK) an important player in transmitting signals from the ECM to the intracellular environment. The MAPK pathway further involves integrin alpha 5 beta 1 integrating external signals to modulate gene expression and cell cycle progression. These pathways allow the integrin to coordinate cellular processes with the broader tissue context.

Integrin alpha 5 beta 1 associates with fibrotic diseases and cancer due to its role in cell adhesion and signaling. In fibrotic diseases such as pulmonary fibrosis altered expression and activity of integrin alpha 5 beta 1 can contribute to excessive ECM deposition and tissue stiffening. Similarly in cancer this integrin influences tumor progression and metastasis by modifying tumor microenvironment interactions. Proteins like fibronectin and transforming growth factor-beta (TGF-beta) intersect with integrin alpha 5 beta 1 activity impacting disease pathogenesis and progression.
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Specifications

Form

Lyophilized

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General info

Function

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site

  1. and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973).. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similarities

Belongs to the integrin alpha chain family.

Post-translational modifications

Proteolytic cleavage by PCSK5 mediates activation of the precursor.

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Product protocols

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Target data

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973).. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGA5

Additional targets

ITGB1
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