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Recombinant human Integrin alpha V + beta 3 protein is a Human Fragment protein, in the 31 to 992 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (AB287921), expandable thumbnail

Key facts

Purity
>=95% SDS-PAGE
Expression system
HEK 293 cells
Tags
Tag free
Applications
SDS-PAGE
Biologically active
No

Sequence

This product is comprised of multiple sequences see

Amino acid sequence 1

F N L D V D S P A E Y S G P E G S Y F G F A V D F F V P S A S S R M F L L V G A P K A N T T Q P G I V E G G Q V L K C D W S S T R R C Q P I E F D A T G N R D Y A K D D P L E F K S H Q W F G A S V R S K Q D K I L A C A P L Y H W R T E M K Q E R E P V G T C F L Q D G T K T V E Y A P C R S Q D I D A D G Q G F C Q G G F S I D F T K A D R V L L G G P G S F Y W Q G Q L I S D Q V A E I V S K Y D P N V Y S I K Y N N Q L A T R T A Q A I F D D S Y L G Y S V A V G D F N G D G I D D F V S G V P R A A R T L G M V Y I Y D G K N M S S L Y N F T G E Q M A A Y F G F S V A A T D I N G D D Y A D V F I G A P L F M D R G S D G K L Q E V G Q V S V S L Q R A S G D F Q T T K L N G F E V F A R F G S A I A P L G D L D Q D G F N D I A I A A P Y G G E D K K G I V Y I F N G R S T G L N A V P S Q I L E G Q W A A R S M P P S F G Y S M K G A T D I D K N G Y P D L I V G A F G V D R A I L Y R A R P V I T V N A G L E V Y P S I L N Q D N K T C S L P G T A L K V S C F N V R F C L K A D G K G V L P R K L N F Q V E L L L D K L K Q K G A I R R A L F L Y S R S P S H S K N M T I S R G G L M Q C E E L I A Y L R D E S E F R D K L T P I T I F M E Y R L D Y R T A A D T T G L Q P I L N Q F T P A N I S R Q A H I L L D C G E D N V C K P K L E V S V D S D Q K K I Y I G D D N P L T L I V K A Q N Q G E G A Y E A E L I V S I P L Q A D F I G V V R N N E A L A R L S C A F K T E N Q T R Q V V C D L G N P M K A G T Q L L A G L R F S V H Q Q S E M D T S V K F D L Q I Q S S N L F D K V S P V V S H K V D L A V L A A V E I R G V S S P D H V F L P I P N W E H K E N P E T E E D V G P V V Q H I Y E L R N N G P S S F S K A M L H L Q W P Y K Y N N N T L L Y I L H Y D I D G P M N C T S D M E I N P L R I K I S S L Q T T E K N D T V A G Q G E R D H L I T K R D L A L S E G D I H T L G C G V A Q C L K I V C Q V G R L D R G K S A I L Y V K S L L W T E T F M N K E N Q N H S Y S L K S S A S F N V I E F P Y K N L P I E D I T N S T L V T T N V T W G I Q P A P M P V

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:15184403, PubMed:22278742, PubMed:28117447). ITGAV:ITGB3 acts as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for Adenovirus type C. (Microbial infection) Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for Coxsackievirus A9 and B1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Additional Targets

ITGB3

Alternative names

Recommended products

Recombinant human Integrin alpha V + beta 3 protein is a Human Fragment protein, in the 31 to 992 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.

Key facts

Purity
>=95% SDS-PAGE
Expression system
HEK 293 cells
Applications
SDS-PAGE
Accession
P06756-1
Animal free
Yes
Species
Human
Reconstitution
Reconstitute in PBS
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence 1

F N L D V D S P A E Y S G P E G S Y F G F A V D F F V P S A S S R M F L L V G A P K A N T T Q P G I V E G G Q V L K C D W S S T R R C Q P I E F D A T G N R D Y A K D D P L E F K S H Q W F G A S V R S K Q D K I L A C A P L Y H W R T E M K Q E R E P V G T C F L Q D G T K T V E Y A P C R S Q D I D A D G Q G F C Q G G F S I D F T K A D R V L L G G P G S F Y W Q G Q L I S D Q V A E I V S K Y D P N V Y S I K Y N N Q L A T R T A Q A I F D D S Y L G Y S V A V G D F N G D G I D D F V S G V P R A A R T L G M V Y I Y D G K N M S S L Y N F T G E Q M A A Y F G F S V A A T D I N G D D Y A D V F I G A P L F M D R G S D G K L Q E V G Q V S V S L Q R A S G D F Q T T K L N G F E V F A R F G S A I A P L G D L D Q D G F N D I A I A A P Y G G E D K K G I V Y I F N G R S T G L N A V P S Q I L E G Q W A A R S M P P S F G Y S M K G A T D I D K N G Y P D L I V G A F G V D R A I L Y R A R P V I T V N A G L E V Y P S I L N Q D N K T C S L P G T A L K V S C F N V R F C L K A D G K G V L P R K L N F Q V E L L L D K L K Q K G A I R R A L F L Y S R S P S H S K N M T I S R G G L M Q C E E L I A Y L R D E S E F R D K L T P I T I F M E Y R L D Y R T A A D T T G L Q P I L N Q F T P A N I S R Q A H I L L D C G E D N V C K P K L E V S V D S D Q K K I Y I G D D N P L T L I V K A Q N Q G E G A Y E A E L I V S I P L Q A D F I G V V R N N E A L A R L S C A F K T E N Q T R Q V V C D L G N P M K A G T Q L L A G L R F S V H Q Q S E M D T S V K F D L Q I Q S S N L F D K V S P V V S H K V D L A V L A A V E I R G V S S P D H V F L P I P N W E H K E N P E T E E D V G P V V Q H I Y E L R N N G P S S F S K A M L H L Q W P Y K Y N N N T L L Y I L H Y D I D G P M N C T S D M E I N P L R I K I S S L Q T T E K N D T V A G Q G E R D H L I T K R D L A L S E G D I H T L G C G V A Q C L K I V C Q V G R L D R G K S A I L Y V K S L L W T E T F M N K E N Q N H S Y S L K S S A S F N V I E F P Y K N L P I E D I T N S T L V T T N V T W G I Q P A P M P V
Accession
P06756
Protein length
Fragment
Predicted molecular weight
106.27 kDa
Amino acids
31 to 992
Nature
Recombinant

Amino acid sequence 2

P N I C T T R G V S S C Q Q C L A V S P M C A W C S D E A L P L G S P R C D L K E N L L K D N C A P E S I E F P V S E A R V L E D R P L S D K G S G D S S Q V T Q V S P Q R I A L R L R P D D S K N F S I Q V R Q V E D Y P V D I Y Y L M D L S Y S M K D D L W S I Q N L G T K L A T Q M R K L T S N L R I G F G A F V D K P V S P Y M Y I S P P E A L E N P C Y D M K T T C L P M F G Y K H V L T L T D Q V T R F N E E V K K Q S V S R N R D A P E G G F D A I M Q A T V C D E K I G W R N D A S H L L V F T T D A K T H I A L D G R L A G I V Q P N D G Q C H V G S D N H Y S A S T T M D Y P S L G L M T E K L S Q K N I N L I F A V T E N V V N L Y Q N Y S E L I P G T T V G V L S M D S S N V L Q L I V D A Y G K I R S K V E L E V R D L P E E L S L S F N A T C L N N E V I P G L K S C M G L K I G D T V S F S I E A K V R G C P Q E K E K S F T I K P V G F K D S L I V Q V T F D C D C A C Q A Q A E P N S H R C N N G N G T F E C G V C R C G P G W L G S Q C E C S E E D Y R P S Q Q D E C S P R E G Q P V C S Q R G E C L C G Q C V C H S S D F G K I T G K Y C E C D D F S C V R Y K G E M C S G H G Q C S C G D C L C D S D W T G Y Y C N C T T R T D T C M S S N G L L C S G R G K C E C G S C V C I Q P G S Y G D T C E K C P T C P D A C T F K K E C V E C K K F D R G A L H D E N T C N R Y C R D E I E S V K E L K D T G K D A V N C T Y K N E D D C V V R F Q Y Y E D S S G K S I L Y V V E E P E C P K G P D
Accession
P05106
Protein length
Fragment
Predicted molecular weight
76.44 kDa
Amino acids
28 to 718
Nature
Recombinant

Specifications

Form
Lyophilized

General info

Function

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:15184403, PubMed:22278742, PubMed:28117447). ITGAV:ITGB3 acts as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428).

Sequence similarities

Belongs to the integrin alpha chain family.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha V+Integrin beta 3 also known as integrin αvβ3 or vitronectin receptor is a heterodimeric protein consisting of the alpha v and beta 3 subunits. It is a transmembrane receptor with an approximate mass of 170 kDa. This integrin plays a role in cell adhesion and signaling by binding to extracellular matrix proteins like vitronectin and fibronectin. You can find it expressed on various cell types including endothelial cells osteoclasts and some cancer cells reflecting its involvement in diverse physiological processes.

Biological function summary

Integrin alpha V+beta 3 acts as an important player in maintaining cellular functions such as migration proliferation and survival. It forms part of a complex that mediates interactions between cells and the extracellular matrix facilitating cellular communication and organization. This protein strengthens the structural support to tissues and influences signal transduction pathways critical for cellular behavior.

Pathways

Integrin alpha V+beta 3 integrates into important cellular pathways including the PI3K/AKT and MAPK pathways. These pathways contribute to cell survival growth and angiogenesis. This protein collaborates with other partners like focal adhesion kinase (FAK) and SRC family kinases highlighting its role in the orchestration of signal transmission necessary for physiological and pathological processes.

Associated diseases and disorders

Integrin alpha V+beta 3 connects to cancer and osteoporosis. It is implicated in tumor angiogenesis and metastasis often found in elevated levels in various cancers. The interaction between this integrin and matrix metalloproteinases (MMPs) facilitates cancer cell invasion. Furthermore its role in osteoclast function links it to osteoporosis where it influences bone resorption contributing to the disease pathology.

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1 product image

  • SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (ab287921), expandable thumbnail

    SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (ab287921)

    SDS-PAGE analysis of ab287921

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