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Recombinant human Integrin alpha V + beta 3 protein is a Human Fragment protein, in the 31 to 992 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (AB287921), expandable thumbnail

Key facts

Purity

>=95% SDS-PAGE

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

Biologically active

No

Sequence

This product is comprised of multiple sequences see

Amino acid sequence 1

F N L D V D S P A E Y S G P E G S Y F G F A V D F F V P S A S S R M F L L V G A P K A N T T Q P G I V E G G Q V L K C D W S S T R R C Q P I E F D A T G N R D Y A K D D P L E F K S H Q W F G A S V R S K Q D K I L A C A P L Y H W R T E M K Q E R E P V G T C F L Q D G T K T V E Y A P C R S Q D I D A D G Q G F C Q G G F S I D F T K A D R V L L G G P G S F Y W Q G Q L I S D Q V A E I V S K Y D P N V Y S I K Y N N Q L A T R T A Q A I F D D S Y L G Y S V A V G D F N G D G I D D F V S G V P R A A R T L G M V Y I Y D G K N M S S L Y N F T G E Q M A A Y F G F S V A A T D I N G D D Y A D V F I G A P L F M D R G S D G K L Q E V G Q V S V S L Q R A S G D F Q T T K L N G F E V F A R F G S A I A P L G D L D Q D G F N D I A I A A P Y G G E D K K G I V Y I F N G R S T G L N A V P S Q I L E G Q W A A R S M P P S F G Y S M K G A T D I D K N G Y P D L I V G A F G V D R A I L Y R A R P V I T V N A G L E V Y P S I L N Q D N K T C S L P G T A L K V S C F N V R F C L K A D G K G V L P R K L N F Q V E L L L D K L K Q K G A I R R A L F L Y S R S P S H S K N M T I S R G G L M Q C E E L I A Y L R D E S E F R D K L T P I T I F M E Y R L D Y R T A A D T T G L Q P I L N Q F T P A N I S R Q A H I L L D C G E D N V C K P K L E V S V D S D Q K K I Y I G D D N P L T L I V K A Q N Q G E G A Y E A E L I V S I P L Q A D F I G V V R N N E A L A R L S C A F K T E N Q T R Q V V C D L G N P M K A G T Q L L A G L R F S V H Q Q S E M D T S V K F D L Q I Q S S N L F D K V S P V V S H K V D L A V L A A V E I R G V S S P D H V F L P I P N W E H K E N P E T E E D V G P V V Q H I Y E L R N N G P S S F S K A M L H L Q W P Y K Y N N N T L L Y I L H Y D I D G P M N C T S D M E I N P L R I K I S S L Q T T E K N D T V A G Q G E R D H L I T K R D L A L S E G D I H T L G C G V A Q C L K I V C Q V G R L D R G K S A I L Y V K S L L W T E T F M N K E N Q N H S Y S L K S S A S F N V I E F P Y K N L P I E D I T N S T L V T T N V T W G I Q P A P M P V

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:15184403, PubMed:22278742, PubMed:28117447). ITGAV:ITGB3 act as a receptor for CD40LG (PubMed:31331973).(Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for Adenovirus type C.(Microbial infection) Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for Coxsackievirus A9 and B1.(Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8.(Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1.(Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1.(Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus.(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Targets

ITGB3

Alternative names

Recommended products

Recombinant human Integrin alpha V + beta 3 protein is a Human Fragment protein, in the 31 to 992 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.

Key facts

Purity

>=95% SDS-PAGE

Expression system

HEK 293 cells

Applications

SDS-PAGE

Accession
P06756-1
Animal free

Yes

Species

Human

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence 1

F N L D V D S P A E Y S G P E G S Y F G F A V D F F V P S A S S R M F L L V G A P K A N T T Q P G I V E G G Q V L K C D W S S T R R C Q P I E F D A T G N R D Y A K D D P L E F K S H Q W F G A S V R S K Q D K I L A C A P L Y H W R T E M K Q E R E P V G T C F L Q D G T K T V E Y A P C R S Q D I D A D G Q G F C Q G G F S I D F T K A D R V L L G G P G S F Y W Q G Q L I S D Q V A E I V S K Y D P N V Y S I K Y N N Q L A T R T A Q A I F D D S Y L G Y S V A V G D F N G D G I D D F V S G V P R A A R T L G M V Y I Y D G K N M S S L Y N F T G E Q M A A Y F G F S V A A T D I N G D D Y A D V F I G A P L F M D R G S D G K L Q E V G Q V S V S L Q R A S G D F Q T T K L N G F E V F A R F G S A I A P L G D L D Q D G F N D I A I A A P Y G G E D K K G I V Y I F N G R S T G L N A V P S Q I L E G Q W A A R S M P P S F G Y S M K G A T D I D K N G Y P D L I V G A F G V D R A I L Y R A R P V I T V N A G L E V Y P S I L N Q D N K T C S L P G T A L K V S C F N V R F C L K A D G K G V L P R K L N F Q V E L L L D K L K Q K G A I R R A L F L Y S R S P S H S K N M T I S R G G L M Q C E E L I A Y L R D E S E F R D K L T P I T I F M E Y R L D Y R T A A D T T G L Q P I L N Q F T P A N I S R Q A H I L L D C G E D N V C K P K L E V S V D S D Q K K I Y I G D D N P L T L I V K A Q N Q G E G A Y E A E L I V S I P L Q A D F I G V V R N N E A L A R L S C A F K T E N Q T R Q V V C D L G N P M K A G T Q L L A G L R F S V H Q Q S E M D T S V K F D L Q I Q S S N L F D K V S P V V S H K V D L A V L A A V E I R G V S S P D H V F L P I P N W E H K E N P E T E E D V G P V V Q H I Y E L R N N G P S S F S K A M L H L Q W P Y K Y N N N T L L Y I L H Y D I D G P M N C T S D M E I N P L R I K I S S L Q T T E K N D T V A G Q G E R D H L I T K R D L A L S E G D I H T L G C G V A Q C L K I V C Q V G R L D R G K S A I L Y V K S L L W T E T F M N K E N Q N H S Y S L K S S A S F N V I E F P Y K N L P I E D I T N S T L V T T N V T W G I Q P A P M P V

Accession

P06756

Protein length

Fragment

Predicted molecular weight

106.27 kDa

Amino acids

31 to 992

Nature

Recombinant

Amino acid sequence 2

P N I C T T R G V S S C Q Q C L A V S P M C A W C S D E A L P L G S P R C D L K E N L L K D N C A P E S I E F P V S E A R V L E D R P L S D K G S G D S S Q V T Q V S P Q R I A L R L R P D D S K N F S I Q V R Q V E D Y P V D I Y Y L M D L S Y S M K D D L W S I Q N L G T K L A T Q M R K L T S N L R I G F G A F V D K P V S P Y M Y I S P P E A L E N P C Y D M K T T C L P M F G Y K H V L T L T D Q V T R F N E E V K K Q S V S R N R D A P E G G F D A I M Q A T V C D E K I G W R N D A S H L L V F T T D A K T H I A L D G R L A G I V Q P N D G Q C H V G S D N H Y S A S T T M D Y P S L G L M T E K L S Q K N I N L I F A V T E N V V N L Y Q N Y S E L I P G T T V G V L S M D S S N V L Q L I V D A Y G K I R S K V E L E V R D L P E E L S L S F N A T C L N N E V I P G L K S C M G L K I G D T V S F S I E A K V R G C P Q E K E K S F T I K P V G F K D S L I V Q V T F D C D C A C Q A Q A E P N S H R C N N G N G T F E C G V C R C G P G W L G S Q C E C S E E D Y R P S Q Q D E C S P R E G Q P V C S Q R G E C L C G Q C V C H S S D F G K I T G K Y C E C D D F S C V R Y K G E M C S G H G Q C S C G D C L C D S D W T G Y Y C N C T T R T D T C M S S N G L L C S G R G K C E C G S C V C I Q P G S Y G D T C E K C P T C P D A C T F K K E C V E C K K F D R G A L H D E N T C N R Y C R D E I E S V K E L K D T G K D A V N C T Y K N E D D C V V R F Q Y Y E D S S G K S I L Y V V E E P E C P K G P D

Accession

P05106

Protein length

Fragment

Predicted molecular weight

76.44 kDa

Amino acids

28 to 718

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:15184403, PubMed:22278742, PubMed:28117447). ITGAV:ITGB3 act as a receptor for CD40LG (PubMed:31331973).

Sequence similarities

Belongs to the integrin alpha chain family.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Integrin alpha V+Integrin beta 3 also known as integrin αvβ3 or vitronectin receptor is a heterodimeric protein consisting of the alpha v and beta 3 subunits. It is a transmembrane receptor with an approximate mass of 170 kDa. This integrin plays a role in cell adhesion and signaling by binding to extracellular matrix proteins like vitronectin and fibronectin. You can find it expressed on various cell types including endothelial cells osteoclasts and some cancer cells reflecting its involvement in diverse physiological processes.

Biological function summary

Integrin alpha V+beta 3 acts as an important player in maintaining cellular functions such as migration proliferation and survival. It forms part of a complex that mediates interactions between cells and the extracellular matrix facilitating cellular communication and organization. This protein strengthens the structural support to tissues and influences signal transduction pathways critical for cellular behavior.

Pathways

Integrin alpha V+beta 3 integrates into important cellular pathways including the PI3K/AKT and MAPK pathways. These pathways contribute to cell survival growth and angiogenesis. This protein collaborates with other partners like focal adhesion kinase (FAK) and SRC family kinases highlighting its role in the orchestration of signal transmission necessary for physiological and pathological processes.

Associated diseases and disorders

Integrin alpha V+beta 3 connects to cancer and osteoporosis. It is implicated in tumor angiogenesis and metastasis often found in elevated levels in various cancers. The interaction between this integrin and matrix metalloproteinases (MMPs) facilitates cancer cell invasion. Furthermore its role in osteoclast function links it to osteoporosis where it influences bone resorption contributing to the disease pathology.

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1 product image

  • SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (ab287921), expandable thumbnail

    SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (ab287921)

    SDS-PAGE analysis of ab287921

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