Recombinant Human Integrin beta 1 protein is a Human Full Length protein, in the 1 to 798 aa range, expressed in Wheat germ and suitable for ELISA, SDS-PAGE, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- ELISA, SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M N L Q P I F W I G L I S S V C C V F A Q T D E N R C L K A N A K S C G E C I Q A G P N C G W C T N S T F L Q E G M P T S A R C D D L E A L K K K G C P P D D I E N P R G S K D I K K N K N V T N R S K G T A E K L K P E D I T Q I Q P Q Q L V L R L R S G E P Q T F T L K F K R A E D Y P I D L Y Y L M D L S Y S M K D D L E N V K S L G T D L M N E M R R I T S D F R I G F G S F V E K T V M P Y I S T T P A K L R N P C T S E Q N C T S P F S Y K N V L S L T N K G E V F N E L V G K Q R I S G N L D S P E G G F D A I M Q V A V C G S L I G W R N V T R L L V F S T D A G F H F A G D G K L G G I V L P N D G Q C H L E N N M Y T M S H Y Y D Y P S I A H L V Q K L S E N N I Q T I F A V T E E F Q P V Y K E L K N L I P K S A V G T L S A N S S N V I Q L I I D A Y N S L S S E V I L E N G K L S E G V T I S Y K S Y C K N G V N G T G E N G R K C S N I S I G D E V Q F E I S I T S N K C P K K D S D S F K I R P L G F T E E V E V I L Q Y I C E C E C Q S E G I P E S P K C H E G N G T F E C G A C R C N E G R V G R H C E C S T D E V N S E D M D A Y C R K E N S S E I C S N N G E C V C G Q C V C R K R D N T N E I Y S G K F C E C D N F N C D R S N G L I C G G N G V C K C R V C E C N P N Y T G S A C D C S L D T S T C E A S N G Q I C N G R G I C E C G V C K C T D P K F Q G Q T C E M C Q T C L G V C A E H K E C V Q C R A F N K G E K K D T C T Q E C S Y F N I T K V E S R D K L P Q P V Q P D P V S H C K E K D V D D C W F Y F T Y S V N G N N E V M V H V V E N P E C P T G P D I I P I V A G V V A G I V L I G L A L L L I W K L L M I I H D R R E F A K F E K E K M N A K W D T G E N P I Y K S A V T T V V N P K Y E G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1 (PubMed:33962943). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973). Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity). ITGA9:ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrins ITGA9:ITGB1 and ITGA4:ITGB1 repress PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via their interaction with SVEP1, thereby inhibit vasocontraction (PubMed:35802072). Isoform 2. Interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). Isoform 5. Isoform 5 displaces isoform 1 in striated muscles. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8. (Microbial infection) Acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Acts as a receptor for Epstein-Barr virus/HHV-4. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus. (Microbial infection) Acts as a receptor for Mammalian reovirus. (Microbial infection) In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. (Microbial infection) Interacts with CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed:32487760). Integrin ITGA3:ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression (PubMed:32487760). (Microbial infection) May serve as a receptor for adhesin A (nadA) of N.meningitidis.
Alternative names
CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA
Recommended products
Recombinant Human Integrin beta 1 protein is a Human Full Length protein, in the 1 to 798 aa range, expressed in Wheat germ and suitable for ELISA, SDS-PAGE, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- ELISA, SDS-PAGE, WB
- Biologically active
- No
- Accession
- P05556-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M N L Q P I F W I G L I S S V C C V F A Q T D E N R C L K A N A K S C G E C I Q A G P N C G W C T N S T F L Q E G M P T S A R C D D L E A L K K K G C P P D D I E N P R G S K D I K K N K N V T N R S K G T A E K L K P E D I T Q I Q P Q Q L V L R L R S G E P Q T F T L K F K R A E D Y P I D L Y Y L M D L S Y S M K D D L E N V K S L G T D L M N E M R R I T S D F R I G F G S F V E K T V M P Y I S T T P A K L R N P C T S E Q N C T S P F S Y K N V L S L T N K G E V F N E L V G K Q R I S G N L D S P E G G F D A I M Q V A V C G S L I G W R N V T R L L V F S T D A G F H F A G D G K L G G I V L P N D G Q C H L E N N M Y T M S H Y Y D Y P S I A H L V Q K L S E N N I Q T I F A V T E E F Q P V Y K E L K N L I P K S A V G T L S A N S S N V I Q L I I D A Y N S L S S E V I L E N G K L S E G V T I S Y K S Y C K N G V N G T G E N G R K C S N I S I G D E V Q F E I S I T S N K C P K K D S D S F K I R P L G F T E E V E V I L Q Y I C E C E C Q S E G I P E S P K C H E G N G T F E C G A C R C N E G R V G R H C E C S T D E V N S E D M D A Y C R K E N S S E I C S N N G E C V C G Q C V C R K R D N T N E I Y S G K F C E C D N F N C D R S N G L I C G G N G V C K C R V C E C N P N Y T G S A C D C S L D T S T C E A S N G Q I C N G R G I C E C G V C K C T D P K F Q G Q T C E M C Q T C L G V C A E H K E C V Q C R A F N K G E K K D T C T Q E C S Y F N I T K V E S R D K L P Q P V Q P D P V S H C K E K D V D D C W F Y F T Y S V N G N N E V M V H V V E N P E C P T G P D I I P I V A G V V A G I V L I G L A L L L I W K L L M I I H D R R E F A K F E K E K M N A K W D T G E N P I Y K S A V T T V V N P K Y E G K
- Accession
- P05556
- Protein length
- Full Length
- Predicted molecular weight
- 115.4 kDa
- Amino acids
- 1 to 798
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1 (PubMed:33962943). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973). Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity). ITGA9:ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrins ITGA9:ITGB1 and ITGA4:ITGB1 repress PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via their interaction with SVEP1, thereby inhibit vasocontraction (PubMed:35802072).
- Sequence similarities
Belongs to the integrin beta chain family.
- Subcellular localisation
- Recycling endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin beta 1 also known as CD29 ITGB1 or integrin β1 is a part of the integrin family of cell surface receptors. This protein plays a role in cell adhesion and signal transduction processes. Integrin beta 1 weighs around 88 kDa and typically expresses on the surface of various cell types including epithelial endothelial and certain blood cells. As a transmembrane receptor it interacts with extracellular matrix components such as fibronectin collagen and laminin facilitating cell-matrix adhesion which is key in maintaining cellular structure and function.
- Biological function summary
Integrin beta 1 functions in cells as a component of heterodimeric complexes with alpha integrin subunits forming adhesion receptors that mediate cellular signaling. These integrins are important for processes like cell migration differentiation proliferation and apoptosis. As they interact with various extracellular ligands integrin beta 1 influences numerous cellular responses particularly in tissue remodeling wound healing and embryonic development.
- Pathways
Integrin beta 1 plays significant roles in the MAPK/ERK and PI3K/Akt signaling pathways both of which are essential for cellular growth and survival signals. It forms connections with associated proteins including focal adhesion kinase (FAK) and talin which participate in mechanotransduction and signal propagation. These interactions influence cellular responses to environmental changes impacting processes such as tissue repair and immune response modulation.
- Associated diseases and disorders
Integrin beta 1 associates with cancer progression and fibrosis disorders. Abnormal expression or function of integrin beta 1 can promote tumor invasion and metastasis making it a target for cancer research and potential therapeutic interventions. Furthermore in fibrosis integrin beta 1 interacts with transforming growth factor-beta (TGF-β) signaling contributing to excessive extracellular matrix production and scarring. Understanding these relationships helps in developing targeted treatments for conditions where integrin beta 1 plays a critical role.
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1 product image
SDS-PAGE - Recombinant Human Integrin beta 1 protein (ab114157)
12.5% SDS-PAGE analysis of ab114157, stained with Coomassie Blue.
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