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AB48750

Recombinant human Interferon alpha 1 protein (Active) (Tag Free)

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(10 Publications)

Recombinant human Interferon alpha 1 protein (Active) (Tag Free) is a Human Full Length protein, in the 24 to 189 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, sELISA, FuncS.

View Alternative Names

IFNA13, IFNA1, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D

2 Images
Sandwich ELISA - Recombinant human Interferon alpha 1 protein (Active) (Tag Free) (AB48750)
  • sELISA

Unknown

Sandwich ELISA - Recombinant human Interferon alpha 1 protein (Active) (Tag Free) (AB48750)

Standard curve for Interferon alpha 1 (Analyte : ab48750); dilution range 1pg/ml to 1μg/ml using Capture Antibody ab20200 at 5μg/ml and Detector Antibody ab84257 at 0.5μg/ml.

SDS-PAGE - Recombinant human Interferon alpha 1 protein (Active) (Tag Free) (AB48750)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Interferon alpha 1 protein (Active) (Tag Free) (AB48750)

3ug of reduced ab48750 on SDS-PAGE, stained with Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

sELISA, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Interferon alpha 1 (ab48750) has the activity of 4 X 105 unit/mg (or 4 X 105 unit/ml).

This activity has been tested by evaluating anti-proliferation with Daudi cells. For Further information see Kim et al., 2009.

Accession

P01562

Animal free

No

Carrier free

Yes

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Concentration determined by Bradford assay.

Endotoxin levels determined by LAL method.

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Sequence info

[{"sequence":"CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE","proteinLength":"Full Length","predictedMolecularWeight":"19.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":189,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01562","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.
Biological function summary

Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.

Pathways

Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.

Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.
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Specifications

Form

Liquid

Additional notes

This protein was purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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General info

Function

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.

Sequence similarities

Belongs to the alpha/beta interferon family.

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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information IFNA1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109213 PubMed38439953

2024

CD169 classical monocyte as an important participant in Graves' ophthalmopathy through CXCL12-CXCR4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongliang Wang,Jie Ling,RongQiang Tan,Huishi Wang,Yixin Qu,Xingyi Li,Jinshan Lin,Qikai Zhang,Qiuling Hu,Zhong Liu,Zhaojing Lu,Yuheng Lin,Li Sun,Dingqiao Wang,Ming Zhou,Zhuoxing Shi,Wuyou Gao,Huijing Ye,Xianchai Lin

JCI insight 8: PubMed36946463

2023

JAK-STAT activation contributes to cytotoxic T cell-mediated basal cell death in human chronic lung allograft dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Aaditya Khatri,Jamie L Todd,Fran L Kelly,Andrew Nagler,Zhicheng Ji,Vaibhav Jain,Simon G Gregory,Kent J Weinhold,Scott M Palmer

Cell reports 41:111606 PubMed36351407

2022

Single-cell transcriptome profiling and chromatin accessibility reveal an exhausted regulatory CD4+ T cell subset in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Guo,Qian Liu,Dandan Zong,Wen Zhang,Zuqi Zuo,Qiaoni Yu,Qing Sha,Lin Zhu,Xuyuan Gao,Jingwen Fang,Jinhui Tao,Quan Wu,Xiaomei Li,Kun Qu

International journal of molecular sciences 23: PubMed36293012

2022

Interactions between the NLRP3-Dependent IL-1β and the Type I Interferon Pathways in Human Plasmacytoid Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dóra Bencze,Tünde Fekete,Walter Pfliegler,Árpád Szöőr,Eszter Csoma,Antónia Szántó,Tünde Tarr,Attila Bácsi,Lajos Kemény,Zoltán Veréb,Kitti Pázmándi

The FEBS journal 289:808-831 PubMed34528388

2021

APOBEC3C, a nucleolar protein induced by genotoxins, is excluded from DNA damage sites.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Constantin,Gilles Dubuis,María Del Carmen Conde-Rubio,Christian Widmann

Molecular cancer research : MCR 19:1398-1411 PubMed34088870

2021

Mapping of mA and Its Regulatory Targets in Prostate Cancer Reveals a METTL3-Low Induction of Therapy Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Kellie A Cotter,John Gallon,Nadine Uebersax,Philip Rubin,Kate D Meyer,Salvatore Piscuoglio,Samie R Jaffrey,Mark A Rubin

eLife 7: PubMed30575519

2018

Genome-wide quantification of the effects of DNA methylation on human gene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Lea,Christopher M Vockley,Rachel A Johnston,Christina A Del Carpio,Luis B Barreiro,Timothy E Reddy,Jenny Tung

Journal of virology 92: PubMed30021900

2018

Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Miyu Moriyama,Manabu Igarashi,Takumi Koshiba,Takashi Irie,Ayato Takada,Takeshi Ichinohe

Journal of immunology (Baltimore, Md. : 1950) 201:451-464 PubMed29848755

2018

Tumor Suppressor p14ARF Enhances IFN-γ-Activated Immune Response by Inhibiting PIAS1 via SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Alagu,Yoko Itahana,Faizal Sim,Sheng-Hao Chao,Xuezhi Bi,Koji Itahana

Scientific reports 7:3594 PubMed28620207

2017

Dengue virus activates cGAS through the release of mitochondrial DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Sun,Karin B Sundström,Jun Jie Chew,Pradeep Bist,Esther S Gan,Hwee Cheng Tan,Kenneth C Goh,Tanu Chawla,Choon Kit Tang,Eng Eong Ooi
View all publications
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