Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) is a Human Fragment protein, in the 28 to 436 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
K N L K S P Q K V E V D I I D D N F I L R W N R S D E S V G N V T F S F D Y Q K T G M D N W I K L S G C Q N I T S T K C N F S S L K L N V Y E E I K L R I R A E K E N T S S W Y E V D S F T P F R K A Q I G P P E V H L E A E D K A I V I H I S P G T K D S V M W A L D G L S F T Y S L V I W K N S S G V E E R I E N I Y S R H K I Y K L S P E T T Y C L K V K A A L L T S W K I G V Y S P V H C I K T T V E N E L P P P E N I E V S V Q N Q N Y V L K W D Y T Y A N M T F Q V Q W L H A F L K R N P G N H L Y K W K Q I P D C E N V K T T Q C V F P Q N V F Q K G I Y L L R V Q A S D G N N T S F W S E E I K F D T E I Q A F L L P P V F N I R S L S D S F H I Y I G A P K Q S G N T P V I Q D Y P L I Y E I I F W E N T S N A E R K I I E K K T D V T V P N L K P L T V Y C V K A R A H T M D E K L N K S S V F S D A V C E K T K P G N T S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986). Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (PubMed:7665574, PubMed:21854986, PubMed:32972995). Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).
Alternative names
Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, Type I interferon receptor 1, CRF2-1, IFNAR, IFNAR1
Recommended products
Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) is a Human Fragment protein, in the 28 to 436 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, Type I interferon receptor 1, CRF2-1, IFNAR, IFNAR1
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P17181-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris buffer
Sequence info
Amino acid sequence
K N L K S P Q K V E V D I I D D N F I L R W N R S D E S V G N V T F S F D Y Q K T G M D N W I K L S G C Q N I T S T K C N F S S L K L N V Y E E I K L R I R A E K E N T S S W Y E V D S F T P F R K A Q I G P P E V H L E A E D K A I V I H I S P G T K D S V M W A L D G L S F T Y S L V I W K N S S G V E E R I E N I Y S R H K I Y K L S P E T T Y C L K V K A A L L T S W K I G V Y S P V H C I K T T V E N E L P P P E N I E V S V Q N Q N Y V L K W D Y T Y A N M T F Q V Q W L H A F L K R N P G N H L Y K W K Q I P D C E N V K T T Q C V F P Q N V F Q K G I Y L L R V Q A S D G N N T S F W S E E I K F D T E I Q A F L L P P V F N I R S L S D S F H I Y I G A P K Q S G N T P V I Q D Y P L I Y E I I F W E N T S N A E R K I I E K K T D V T V P N L K P L T V Y C V K A R A H T M D E K L N K S S V F S D A V C E K T K P G N T S K
- Accession
- Protein length
Fragment
- Predicted molecular weight
73.8 kDa
- Amino acids
28 to 436
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986). Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (PubMed:7665574, PubMed:21854986, PubMed:32972995). Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).
- Sequence similarities
Belongs to the type II cytokine receptor family.
- Post-translational modifications
Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985).
- Subcellular localisation
Late endosome, Lysosome
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
- Activity summary
Interferon alpha/beta receptor 1 also known as IFNAR1 is an important component of the interferon receptor complex which includes the interferon beta receptor (IFNBR) and the broader class of interferon receptors. IFNAR1 is a transmembrane protein with a molecular mass of approximately 100 kDa. It shows expression in various tissues but predominantly in cells of the immune system. This expression pattern enables IFNAR1 to play a central role in mediating responses to interferon alpha and beta which are cytokines important in the immune response.
- Biological function summary
IFNAR1 partners with interferon alpha receptor 2 (IFNAR2) to form a functional receptor complex. This complex binds interferons alpha and beta triggering signal transduction cascades vital for antiviral defense. Through this interaction IFNAR1 facilitates cellular immune responses including the activation of antiviral genes and modulation of cell proliferation. IFNAR1's role extends beyond immediate immune response influencing processes like cell differentiation and apoptosis.
- Pathways
IFNAR1 is a participant in the JAK-STAT signaling pathway a significant pathway for transmitting cytokine signals. Upon interferon binding it activates the associated Janus kinases (JAKs) which in turn phosphorylate signal transducers and activators of transcription (STATs) specifically STAT1 and STAT2. This chain of events results in the transcription of interferon-stimulated genes that provide antiviral defenses and promote immune regulation. IFNAR1's engagement in these pathways makes it an integral part of immune system communication.
- Associated diseases and disorders
IFNAR1 associates strongly with autoimmune diseases and viral infections. In lupus an autoimmune disorder dysregulation in interferon signaling involving IFNAR1 contributes to disease progression. Moreover in viral infections like hepatitis C the response of IFNAR1 to interferon therapies can influence treatment outcomes. Understanding these connections facilitates therapeutic strategies that target interferon pathways for improved disease management.
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2 product images
SDS-PAGE - Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) (ab219671)
SDS-PAGE of reduced ab219671 stained overnight with Coomassie Blue. The protein migrates as 105-120 kDa due to glycosylation.
Biological Activity - Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) (ab219671)
Loaded Human IFN-alpha 1, His Tag (ab219671) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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