Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) is a Human Fragment protein, in the 28 to 436 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
K N L K S P Q K V E V D I I D D N F I L R W N R S D E S V G N V T F S F D Y Q K T G M D N W I K L S G C Q N I T S T K C N F S S L K L N V Y E E I K L R I R A E K E N T S S W Y E V D S F T P F R K A Q I G P P E V H L E A E D K A I V I H I S P G T K D S V M W A L D G L S F T Y S L V I W K N S S G V E E R I E N I Y S R H K I Y K L S P E T T Y C L K V K A A L L T S W K I G V Y S P V H C I K T T V E N E L P P P E N I E V S V Q N Q N Y V L K W D Y T Y A N M T F Q V Q W L H A F L K R N P G N H L Y K W K Q I P D C E N V K T T Q C V F P Q N V F Q K G I Y L L R V Q A S D G N N T S F W S E E I K F D T E I Q A F L L P P V F N I R S L S D S F H I Y I G A P K Q S G N T P V I Q D Y P L I Y E I I F W E N T S N A E R K I I E K K T D V T V P N L K P L T V Y C V K A R A H T M D E K L N K S S V F S D A V C E K T K P G N T S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa) (PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:2153461, PubMed:21854986, PubMed:24075985, PubMed:31270247, PubMed:33252644, PubMed:35442418, PubMed:7813427). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response (PubMed:10049744, PubMed:21854986, PubMed:7665574). Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another (PubMed:21854986, PubMed:32972995, PubMed:7665574, PubMed:7813427). The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (PubMed:21854986, PubMed:32972995, PubMed:7526154, PubMed:7665574, PubMed:7813427). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes (PubMed:19561067, PubMed:21854986, PubMed:32972995, PubMed:7665574, PubMed:7813427, PubMed:9121453). Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).
Alternative names
IFNAR, IFNAR1, Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, Type I interferon receptor 1, CRF2-1
Recommended products
Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) is a Human Fragment protein, in the 28 to 436 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P17181-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris buffer
Sequence info
Amino acid sequence
- K N L K S P Q K V E V D I I D D N F I L R W N R S D E S V G N V T F S F D Y Q K T G M D N W I K L S G C Q N I T S T K C N F S S L K L N V Y E E I K L R I R A E K E N T S S W Y E V D S F T P F R K A Q I G P P E V H L E A E D K A I V I H I S P G T K D S V M W A L D G L S F T Y S L V I W K N S S G V E E R I E N I Y S R H K I Y K L S P E T T Y C L K V K A A L L T S W K I G V Y S P V H C I K T T V E N E L P P P E N I E V S V Q N Q N Y V L K W D Y T Y A N M T F Q V Q W L H A F L K R N P G N H L Y K W K Q I P D C E N V K T T Q C V F P Q N V F Q K G I Y L L R V Q A S D G N N T S F W S E E I K F D T E I Q A F L L P P V F N I R S L S D S F H I Y I G A P K Q S G N T P V I Q D Y P L I Y E I I F W E N T S N A E R K I I E K K T D V T V P N L K P L T V Y C V K A R A H T M D E K L N K S S V F S D A V C E K T K P G N T S K
- Accession
- P17181
- Protein length
- Fragment
- Predicted molecular weight
- 73.8 kDa
- Amino acids
- 28 to 436
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa) (PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:2153461, PubMed:21854986, PubMed:24075985, PubMed:31270247, PubMed:33252644, PubMed:35442418, PubMed:7813427). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response (PubMed:10049744, PubMed:21854986, PubMed:7665574). Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another (PubMed:21854986, PubMed:32972995, PubMed:7665574, PubMed:7813427). The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (PubMed:21854986, PubMed:32972995, PubMed:7526154, PubMed:7665574, PubMed:7813427). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes (PubMed:19561067, PubMed:21854986, PubMed:32972995, PubMed:7665574, PubMed:7813427, PubMed:9121453). Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).
- Sequence similarities
Belongs to the type II cytokine receptor family.
- Post-translational modifications
Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985).
- Subcellular localisation
- Late endosome, Lysosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon alpha/beta receptor 1 also known as IFNAR1 is an important component of the interferon receptor complex which includes the interferon beta receptor (IFNBR) and the broader class of interferon receptors. IFNAR1 is a transmembrane protein with a molecular mass of approximately 100 kDa. It shows expression in various tissues but predominantly in cells of the immune system. This expression pattern enables IFNAR1 to play a central role in mediating responses to interferon alpha and beta which are cytokines important in the immune response.
- Biological function summary
IFNAR1 partners with interferon alpha receptor 2 (IFNAR2) to form a functional receptor complex. This complex binds interferons alpha and beta triggering signal transduction cascades vital for antiviral defense. Through this interaction IFNAR1 facilitates cellular immune responses including the activation of antiviral genes and modulation of cell proliferation. IFNAR1's role extends beyond immediate immune response influencing processes like cell differentiation and apoptosis.
- Pathways
IFNAR1 is a participant in the JAK-STAT signaling pathway a significant pathway for transmitting cytokine signals. Upon interferon binding it activates the associated Janus kinases (JAKs) which in turn phosphorylate signal transducers and activators of transcription (STATs) specifically STAT1 and STAT2. This chain of events results in the transcription of interferon-stimulated genes that provide antiviral defenses and promote immune regulation. IFNAR1's engagement in these pathways makes it an integral part of immune system communication.
- Associated diseases and disorders
IFNAR1 associates strongly with autoimmune diseases and viral infections. In lupus an autoimmune disorder dysregulation in interferon signaling involving IFNAR1 contributes to disease progression. Moreover in viral infections like hepatitis C the response of IFNAR1 to interferon therapies can influence treatment outcomes. Understanding these connections facilitates therapeutic strategies that target interferon pathways for improved disease management.
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2 product images
SDS-PAGE - Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) (ab219671)
SDS-PAGE of reduced ab219671 stained overnight with Coomassie Blue. The protein migrates as 105-120 kDa due to glycosylation.
Biological Activity - Recombinant Human Interferon alpha/beta receptor 1 protein (Fc Chimera) (ab219671)
Loaded Human IFN-alpha 1, His Tag (ab219671) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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