Recombinant Human Interferon gamma protein (Tag Free)
- Bioactive
- What is this?
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(2 Publications)
Recombinant Human Interferon gamma protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, Biological Activity, FuncS.
View Alternative Names
Interferon gamma, IFN-gamma, Immune interferon, IFNG
- WB
Unknown
Western blot - Recombinant Human Interferon gamma protein (Tag Free) (AB51240)
All lanes:
Western blot - Anti-Interferon gamma antibody (<a href='/en-us/products/primary-antibodies/interferon-gamma-antibody-ab25101'>ab25101</a>) at 1 µg/mL
All lanes:
Western blot - Recombinant Human Interferon gamma protein (Tag Free) (ab51240) at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 19 kDa
true
Exposure time: 30s
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Interferon gamma protein (Tag Free) (AB51240)
ab51240 on 14% SDS-PAGE.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.
Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
Specifications
Form
Liquid
Additional notes
Recombinant human Interferon gamma was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
General info
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
Sequence similarities
Belongs to the type II (or gamma) interferon family.
Post-translational modifications
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Target data
Publications (2)
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Journal of the American Chemical Society 145:20432-20441 PubMed37677157
2023
Applications
Unspecified application
Species
Unspecified reactive species
Biologicals : journal of the International Association of Biological Standardization 45:52-60 PubMed27810255
2016
Applications
Unspecified application
Species
Unspecified reactive species
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