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Recombinant Human Interferon gamma protein (Active) is a Human Full Length protein, in the 24 to 166 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

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Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biologically active

Yes

Sequence

Q D P Y V Q E A E N L K K Y F N A G H S D V A D N G T L F L G I L K N W K E E S D R K I M Q S Q I V S F Y F K L F K N F K D D Q S I Q K S V E T I K E D M N V K F F N S N K K K R D D F E K L T N Y S V T D L N V Q R K A I H E L I Q V M A E L S P A A K T G K R K R S Q M L F R G R R A S Q

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant Human Interferon gamma protein (Active) is a Human Full Length protein, in the 24 to 166 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biological activity

Fully biologically active determined by the dose dependent reduction in cytopathic effect of viral infection in A549 cells. ED50 for this effect is 0.30 ng/ml corresponding to a specific activity of 3.33 x 106 units/mg.

Predicted molecular weight

16.83 kDa

Amino acids

24 to 166

Sequence

Q D P Y V Q E A E N L K K Y F N A G H S D V A D N G T L F L G I L K N W K E E S D R K I M Q S Q I V S F Y F K L F K N F K D D Q S I Q K S V E T I K E D M N V K F F N S N K K K R D D F E K L T N Y S V T D L N V Q R K A I H E L I Q V M A E L S P A A K T G K R K R S Q M L F R G R R A S Q

Accession
P01579-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with Phosphate Buffered Saline.Reconstituted protein stable at -80C for 12 months or 4C for 1 week. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

>= 95 % HPLC.

General info

Function

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

Sequence similarities

Belongs to the type II (or gamma) interferon family.

Post-translational modifications

Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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