Recombinant human Interferon gamma protein (Active) is a Human Full Length protein, in the 1 to 144 aa range with >98% purity, < 1 EU/µg endotoxin level and suitable for western blot, SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab9659 protein is 16.8 kDa.
- Save time and ensure accurate results- use our IFN-gamma protein as a control
- Optimal protein bioactivity and stability
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- Cells 12:2022MiR-302a Regenerates Human Corneal Endothelial Cells against IFN-γ-Induced Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSe-Hie Park,Jin-Sun Hwang,Sun-Hee Oh,Young-Joo ShinPubMed 36611829
- Frontiers in psychiatry 13:8362172022Interferon-γ exposure of human iPSC-derived neurons alters major histocompatibility complex I and synapsin protein expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Pavlinek,Rugile Matuleviciute,Laura Sichlinger,Lucia Dutan Polit,Nikolaos Armeniakos,Anthony Christopher Vernon,Deepak Prakash SrivastavaPubMed 36186864
- Science advances 6:eaay95062020Interferon-γ signaling in human iPSC-derived neurons recapitulates neurodevelopmental disorder phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine Warre-Cornish et. al.PubMed 32875100
- Nature structural & molecular biology 23:1101-11102016RNA-binding protein CPEB1 remodels host and viral RNA landscapes.Applications:FuncSReactive species:HumanRanjan Batra,Thomas J Stark,Elizabeth Clark,Jean-Philippe Belzile,Emily C Wheeler,Brian A Yee,Hui Huang,Chelsea Gelboin-Burkhart,Stephanie C Huelga,Stefan Aigner,Brett T Roberts,Tomas J Bos,Shashank Sathe,John Paul Donohue,Frank Rigo,Manuel Ares,Deborah H Spector,Gene W YeoPubMed 27775709
- The Journal of biological chemistry 282:22414-252007Oxidative stress modulates complement factor H expression in retinal pigmented epithelial cells by acetylation of FOXO3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihao Wu et. al.PubMed 17558024
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC, WB
- Biologically active
- Yes
Amino acid sequence
M Q D P Y V K E A E N L K K Y F N A G H S D V A D N G T L F L G I L K N W K E E S D R K I M Q S Q I V S F Y F K L F K N F K D D Q S I Q K S V E T I K E D M N V K F F N S N K K K R D D F E K L T N Y S V T D L N V Q R K A I H E L I Q V M A E L S P A A K T G K R K R S Q M L F Q G R R A S Q
Consider this alternative
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
Alternative names
Interferon gamma, IFN-gamma, Immune interferon, IFNG
Recommended products
Recombinant human Interferon gamma protein (Active) is a Human Full Length protein, in the 1 to 144 aa range with >98% purity, < 1 EU/µg endotoxin level and suitable for western blot, SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab9659 protein is 16.8 kDa.
- Save time and ensure accurate results- use our IFN-gamma protein as a control
- Optimal protein bioactivity and stability
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC, WB
- Biologically active
- Yes
- Biological activity
- The ED50 determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.
- Accession
- P01579-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- reconstitute with PBS, pH 8.0 at 100µL
- Concentration
- Storage buffer
Constituents: PBS
Sequence info
Amino acid sequence
- M Q D P Y V K E A E N L K K Y F N A G H S D V A D N G T L F L G I L K N W K E E S D R K I M Q S Q I V S F Y F K L F K N F K D D Q S I Q K S V E T I K E D M N V K F F N S N K K K R D D F E K L T N Y S V T D L N V Q R K A I H E L I Q V M A E L S P A A K T G K R K R S Q M L F Q G R R A S Q
- Accession
- P01579
- Protein length
- Full Length
- Predicted molecular weight
- 16.8 kDa
- Amino acids
- 1 to 144
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>98%% HPLC analyses. Sterile filtered.
General info
- Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
- Sequence similarities
Belongs to the type II (or gamma) interferon family.
- Post-translational modifications
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our recombinant bioactive human IFN-gamma protein ab9659 as a positive control in western blot, SDS-PAGE and HPLC. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation.
Human IFN gamma does not show cross-reactivity with mouse.
Check out our protein gel staining guide for SDS-PAGE here
Check out of western blot protocol for more information here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
- Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.
- Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
- Associated diseases and disorders
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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2 product images
Immunoprecipitation - Recombinant human Interferon gamma protein (Active) (ab9659)
CXCL11 was immunoprecipitated from 0.35mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with Anti-CXCL11 antibody [EPR21755-173] ab216157 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-CXCL11 antibody [EPR21755-173] ab216157 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: THP-1 (human monocytic leukemia cell line) treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate 10ug (Input).
Lane 2: Anti-CXCL11 antibody [EPR21755-173] ab216157 IP in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CXCL11 antibody [EPR21755-173] ab216157 in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST
All lanes: Immunoprecipitation - Recombinant human Interferon gamma protein (Active) (ab9659)
Developed using the ECL technique.
Exposure time: 30s
Western blot - Recombinant human Interferon gamma protein (Active) (ab9659)
Lane 1: Western blot - Anti-Interferon gamma antibody (Anti-Interferon gamma antibody ab9657)
Lanes 2 - 12: Western blot - Anti-Interferon gamma antibody (Anti-Interferon gamma antibody ab9657) at 0.1 µg/mL
All lanes: Western blot - Recombinant human Interferon gamma protein (Active) (ab9659)
Performed under non-reducing conditions.
Predicted band size: 19 kDa
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