Recombinant Human Interferon omega Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
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Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- sELISA, SDS-PAGE
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Interferon omega-1, Interferon alpha-II-1, IFNW1
Recombinant Human Interferon omega Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- sELISA, SDS-PAGE
- Biologically active
- No
- Accession
- P05000-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- P05000
- Protein length
- Full Length
- Predicted molecular weight
- 22.2 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Sequence similarities
Belongs to the alpha/beta interferon family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFNW1 also known as interferon omega is a type I interferon with a mass of approximately 23 kDa. Mechanically this protein functions in the immune response by inducing antiviral state in cells. It is expressed in various tissues including immune cells like leukocytes. IFNW1 binds to the specific interferon alpha/beta receptor (IFNAR) to exert its effects. This interaction triggers signaling cascades that activate the transcription of interferon-stimulated genes which are critical in antiviral defense.
- Biological function summary
IFNW1 plays important roles in modulating the immune response and controlling viral infections. It is not part of a known protein complex but interacts with IFNAR to activate signaling pathways. It enhances the expression of antiviral proteins and cells' ability to combat pathogen intrusions. Beyond antiviral activity IFNW1 can influence cell differentiation and the immune system's surveillance capabilities ensuring its response against threats is effective.
- Pathways
IFNW1 contributes to the JAK-STAT signaling pathway and the innate immune response. In the JAK-STAT pathway it activates key proteins such as JAK1 and TYK2 which lead to phosphorylation and activation of STAT proteins. These activated STAT proteins then translocate to the nucleus and promote transcription of genes involved in antiviral defense. IFNW1 shares pathways with other interferons like IFNA and IFNB making it an integral part of the cytokine signaling network that regulates immune function.
- Associated diseases and disorders
IFNW1 has associations with viral infections and autoimmune disorders like systemic lupus erythematosus (SLE). Its role in antiviral defense positions IFNW1 as a target for understanding virus-host interactions that contribute to pathogenesis. In autoimmune disorders like SLE the dysregulation of interferon signaling including that involving IFNW1 links to the aberrant immune activation seen in these conditions. IFNW1's interactions with IFNA and IFNB highlight its involvement in pathways that can drive disease states emphasizing its relevance in disease research and potential therapeutic approaches.
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2 product images
SDS-PAGE - Recombinant Human Interferon omega Protein Standard (His tag) (ab316543)
SDS-PAGE analysis of ab316543 under reducing conditions for 2ug protein.
Sandwich ELISA - Recombinant Human Interferon omega Protein Standard (His tag) (ab316543)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
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