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AB276557

Recombinant Human Intrinsic Factor protein (His tag)

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Recombinant Human Intrinsic Factor protein (His tag) is a Human Full Length protein, in the 1 to 417 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

GIF, IFMH, CBLIF, Cobalamin binding intrinsic factor, Gastric intrinsic factor, Intrinsic factor, IF, INF

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SDS-PAGE - Recombinant Human Intrinsic Factor protein (His tag) (AB276557)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Intrinsic Factor protein (His tag) (AB276557)

SDS-PAGE analysis of ab276557

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P27352

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAWFALYLLSLLWATAGTSTQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAILALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLALTCMYNKIPVGSEEGYRSLFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPSNPGPGPTSASNITVIYTINNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY","proteinLength":"Full Length","predictedMolecularWeight":"44.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":417,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P27352","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Intrinsic Factor also known as gastric intrinsic factor is a protein important for the absorption of vitamin B12. Produced by parietal cells in the gastric mucosa intrinsic factor has an approximate molecular weight of 45 kDa. It is secreted in the stomach and then travels to the small intestine. Intrinsic factor specifically binds to vitamin B12 forming a complex that enables the vitamin's uptake in the ileum. The presence of anti-intrinsic factor antibodies can impede its function playing a role in clinical diagnostics for conditions like pernicious anemia.
Biological function summary

The role of intrinsic factor is closely linked with vitamin B12 metabolism. As part of a transport complex it safeguards vitamin B12 from degradation as it moves through the gastrointestinal tract. In the absence of intrinsic factor vitamin B12 cannot effectively bind and undergo absorption in the ileum. The failure in this binding and absorption process often leads to severe nutritional deficiencies manifesting as a precursor to various hematological abnormalities.

Pathways

Intrinsic factor operates in the vitamin B12 absorption pathway. This pathway involves interactions with several proteins including cubilin and megalin which serve as part of the receptor-mediated endocytosis process in the ileum. Additionally the transcobalamin protein further facilitates the distribution of vitamin B12 within the bloodstream post-absorption highlighting the interconnected network between these proteins and intrinsic factor.

Intrinsic factor is strongly implicated in pernicious anemia. This condition arises when the body produces anti-intrinsic factor antibodies leading to vitamin B12 deficiency. The deficiency affects the production and maturation of red blood cells manifesting as macrocytic anemia. Moreover a lack of intrinsic factor has been associated with neurological disorders because vitamin B12 is critical for proper neural function. Without sufficient B12 myelin sheath formation and neuronal maintenance suffer which can lead to neurological complications.
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Specifications

Form

Lyophilized

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General info

Function

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.

Sequence similarities

Belongs to the eukaryotic cobalamin transport proteins family.

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Product protocols

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Target data

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.
See full target information CBLIF
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