Recombinant human IRAK-1 protein is a Human Fragment protein, in the 194 to 712 aa range, expressed in Insect, with >85% purity and suitable for SDS-PAGE, FuncS, WB.
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Publications
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- International journal of molecular sciences 21:2020Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMárió Miczi et. al.PubMed 33333742
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Insect cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
Amino acid sequence
R P F P F C W P L C E I S R G T H N F S E E L K I G E G G F G C V Y R A V M R N T V Y A V K R L K E N A D L E W T A V K Q S F L T E V E Q L S R F R H P N I V D F A G Y C A Q N G F Y C L V Y G F L P N G S L E D R L H C Q T Q A C P P L S W P Q R L D I L L G T A R A I Q F L H Q D S P S L I H G D I K S S N V L L D E R L T P K L G D F G L A R F S R F A G S S P S Q S S M V A R T Q T V R G T L A Y L P E E Y I K T G R L A V D T D T F S F G V V V L E T L A G Q R A V K T H G A R T K Y L K D L V E E E A E E A G V A L R S T Q S T L Q A G L A A D A W A A P I A M Q I Y K K H L D P R P G P C P P E L G L G L G Q L A C C C L H R R A K R R P P M T Q V Y E R L E K L Q A V V A G V P G H S E A A S C I P P S P Q E N S Y V S S T G R A H S G A A P W Q P L A A P S G A S A Q A A E Q L Q R G P N Q P V E S D E S L G G L S A A L R S W H L T P S C P L D P A P L R E A G C P Q G D T A G E S S W G S G P G S R P T A V E G L A L G S S A S S S S E P P Q I I I N P A R Q K M V Q K L A L Y E D G A L D S L Q L L S S S S L P G L G L E Q D R Q G P E E S D E F Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
Alternative names
IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1
Recommended products
Recombinant human IRAK-1 protein is a Human Fragment protein, in the 194 to 712 aa range, expressed in Insect, with >85% purity and suitable for SDS-PAGE, FuncS, WB.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Insect cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
- Biological activity
- The specific activity of IRAK1 was determined to be 27 nmol/min/mg.
- Accession
- P51617-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- R P F P F C W P L C E I S R G T H N F S E E L K I G E G G F G C V Y R A V M R N T V Y A V K R L K E N A D L E W T A V K Q S F L T E V E Q L S R F R H P N I V D F A G Y C A Q N G F Y C L V Y G F L P N G S L E D R L H C Q T Q A C P P L S W P Q R L D I L L G T A R A I Q F L H Q D S P S L I H G D I K S S N V L L D E R L T P K L G D F G L A R F S R F A G S S P S Q S S M V A R T Q T V R G T L A Y L P E E Y I K T G R L A V D T D T F S F G V V V L E T L A G Q R A V K T H G A R T K Y L K D L V E E E A E E A G V A L R S T Q S T L Q A G L A A D A W A A P I A M Q I Y K K H L D P R P G P C P P E L G L G L G Q L A C C C L H R R A K R R P P M T Q V Y E R L E K L Q A V V A G V P G H S E A A S C I P P S P Q E N S Y V S S T G R A H S G A A P W Q P L A A P S G A S A Q A A E Q L Q R G P N Q P V E S D E S L G G L S A A L R S W H L T P S C P L D P A P L R E A G C P Q G D T A G E S S W G S G P G S R P T A V E G L A L G S S A S S S S E P P Q I I I N P A R Q K M V Q K L A L Y E D G A L D S L Q L L S S S S L P G L G L E Q D R Q G P E E S D E F Q S
- Accession
- P51617
- Protein length
- Fragment
- Predicted molecular weight
- 76 kDa
- Amino acids
- 194 to 712
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
- Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
- Post-translational modifications
Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IRAK-1 also known as Interleukin-1 Receptor-Associated Kinase 1 is a serine/threonine kinase that plays a role in the immune response. With a molecular mass around 76 kDa this protein is heavily expressed in immune cells such as macrophages and monocytes. IRAK-1 is an important player in the signal transduction process following the activation of the Toll-like and interleukin-1 receptor (IL-1R) families. The kinase activity of IRAK-1 occurs when it associates with the receptor complex transmitting signals that activate downstream pathways.
- Biological function summary
IRAK-1 mediates the inflammatory response by promoting the production of cytokines and other inflammatory mediators. It is a component of the Myddosome a multi-protein complex that assembles upon receptor activation and includes MyD88 an adaptor protein needed for signal transduction. IRAK-1 phosphorylates other targets and itself leading to its own degradation which is an important step for turning off the signaling and preventing prolonged activation. Its precise activity modulation ensures balanced immune responses and prevents overreaction that could cause tissue damage.
- Pathways
IRAK-1 functions in the IL-1 and Toll-like receptor signaling pathways both of which are important for innate immunity. In these pathways IRAK-1 interacts with key proteins such as TRAF6 which transduces signals leading to NF-κB and MAPK activation critical for inflammatory gene expression. Its role in these pathways highlights the importance of proper function and regulation as deficiencies or dysregulation can result in inappropriate immune responses.
- Associated diseases and disorders
IRAK-1 has been connected to autoimmune diseases such as rheumatoid arthritis and certain cancers. This protein affects how the immune system responds to inflammation and can influence disease progression through its regulatory actions. For instance alterations in IRAK-1 activity or expression can lead to an increase in pro-inflammatory cytokines that exacerbate conditions like rheumatoid arthritis. Additionally in cancer pathways influenced by IRAK-1 can affect tumor growth and metastasis. Its interaction with proteins such as MyD88 and TRAF6 within these pathological contexts underlines its importance as a potential target for therapeutic intervention.
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2 product images
SDS-PAGE - Recombinant human IRAK-1 protein (ab268679)
Recombinant Human IRAK1 protein purity determined using SDS-PAGE analysis.
Functional Studies - Recombinant human IRAK-1 protein (ab268679)
Recombinant Human IRAK1 protein activity determined to be 27 nmol/min/mg as per activity assay protocol.
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