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AB190994

Recombinant human IRE1b protein (GST tag N-Terminus)

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Recombinant human IRE1b protein (GST tag N-Terminus) is a Human Fragment protein, in the 499 to 926 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)
  • FuncS

Supplier Data

Functional Studies - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)

The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.

Functional Studies - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)
  • FuncS

Unknown

Functional Studies - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)

The specific activity of IRE1b (ab190994) was determined to be 2.4 nmol/min/mg as per activity assay protocol and was equivalent to 2.3 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)

The purity of ab190994 was determined to be >95% by densitometry, approx MW ~80 kDa.

SDS-PAGE - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human IRE1b protein (GST tag N-Terminus) (AB190994)

SDS PAGE analysis of ab190994

Key facts

Purity

>95% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.

Accession

Q76MJ5-1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"KQAQPLDDPEAEQLTVVGKISFNPKDVLGRGAGGTFVFRGQFEGRAVAVKRLLRECFGLVRREVQLLQESDRHPNVLRYFCTERGPQFHYIALELCRASLQEYVENPDLDRGGLEPEVVLQQLMSGLAHLHSLHIVHRDLKPGNILITGPDSQGLGRVVLSDFGLCKKLPAGRCSFSLHSGIPGTEGWMAPELLQLLPPDSPTSAVDIFSAGCVFYYVLSGGSHPFGDSLYRQANILTGAPCLAHLEEEVHDKVVARDLVGAMLSPLPQPRPSAPQVLAHPFFWSRAKQLQFFQDVSDWLEKESEQEPLVRALEAGGCAVVRDNWHEHISMPLQTDLRKFRSYKGTSVRDLLRAVRNKKHHYRELPVEVRQALGQVPDGFVQYFTNRFPRLLLHTHRAMRSCASESLFLPYYPPDSEARRPCPGATGR","proteinLength":"Fragment","predictedMolecularWeight":"80 kDa","actualMolecularWeight":null,"aminoAcidEnd":926,"aminoAcidStart":499,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q76MJ5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IRE1β also known as ERN1B is a transmembrane endoribonuclease with a mass of approximately 110 kDa. It belongs to the IRE1 family and acts as an important sensor of the unfolded protein response (UPR) pathway. IRE1β is expressed mainly in epithelial cells of the intestine with some presence in lungs and liver tissues. The protein comprises a luminal domain that detects misfolded proteins along with a kinase and RNase domain that initiates downstream signaling pathways.
Biological function summary

IRE1β plays a significant role in cellular stress responses by regulating mRNA splicing and degradation especially during endoplasmic reticulum (ER) stress events. It operates as a part of a complex signal transduction mechanism that maintains cellular homeostasis under stress. In particular upon activation IRE1β engages in unconventional splicing of the transcription factor XBP1 mRNA which in turn upregulates genes involved in protein folding and ER-associated degradation.

Pathways

IRE1β functions as a central component in the UPR pathway influencing ER homeostasis and adaptive stress responses. It works closely with proteins such as PERK and ATF6 which are other transducers of the UPR pathway. This integrated response helps cells to recover from stress by improving protein folding degradation and overall ER function. Additionally IRE1β and its related pathways link to the inflammatory response pathways linking protein misfolding with inflammation signaling.

IRE1β is connected to conditions like inflammatory bowel disease (IBD) and cystic fibrosis. IRE1β modulates intestinal epithelial response to inflammation affecting the pathogenesis of IBD. It also influences the aggregation of misfolded proteins that characterize cystic fibrosis directly associating with proteins like CFTR whose malfunction leads to disease symptoms. Proper regulation of IRE1β activity is vital for maintaining intestinal health and preventing chronic inflammation.
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General info

Function

Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Post-translational modifications

Autophosphorylated.

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Product protocols

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Target data

Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.
See full target information ERN2

Alternative Names

IRE2, ERN2, Serine/threonine-protein kinase/endoribonuclease IRE2, Endoplasmic reticulum-to-nucleus signaling 2, Inositol-requiring protein 2, Ire1-beta, hIRE2p, IRE1b
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