Recombinant Human IRF3 protein is a Human Full Length protein, in the 2 to 427 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His-T7 tag N-Terminus
SDS-PAGE
No
M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G E F G T P K P R I L P W L V S Q L D L G Q L E G V A W V N K S R T R F R I P W K H G L R Q D A Q Q E D F G I F Q A W A E A T G A Y V P G R D K P D L P T W K R N F R S A L N R K E G L R L A E D R S K D P H D P H K I Y E F V N S G V G D F S Q P D T S P D T N G G G S T S D T Q E D I L D E L L G N M V L A P L P D P G P P S L A V A P E P C P Q P L R S P S L D N P T P F P N L G P S E N P L K R L L V P G E E W E F E V T A F Y R G R Q V F Q Q T I S C P E G L R L V G S E V G D R T L P G W P V T L P D P G M S L T D R G V M S Y V R H V L S C L G G G L A L W R A G Q W L W A Q R L G H C H T Y W A V S E E L L P N S G H G P D G E V P K D K E G G V F D L G P F I V D L I T F T E G S G R S P R Y A L W F C V G E S W P Q D Q P W T K R L V M V K V V P T C L R A L V E M A R V G G A S S L E N T V D L H I S N S H P L S L T S D Q Y K A Y L Q D L V E G M D F Q G P G E S
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:24049179, PubMed:25636800, PubMed:27302953, PubMed:31340999, PubMed:36603579, PubMed:8524823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995, PubMed:36603579, PubMed:8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148, PubMed:36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Interferon regulatory factor 3, IRF-3, IRF3
Recombinant Human IRF3 protein is a Human Full Length protein, in the 2 to 427 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His-T7 tag N-Terminus
SDS-PAGE
No
No
Human
pH: 8
Constituents: 0.32% Tris HCl
M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G E F G T P K P R I L P W L V S Q L D L G Q L E G V A W V N K S R T R F R I P W K H G L R Q D A Q Q E D F G I F Q A W A E A T G A Y V P G R D K P D L P T W K R N F R S A L N R K E G L R L A E D R S K D P H D P H K I Y E F V N S G V G D F S Q P D T S P D T N G G G S T S D T Q E D I L D E L L G N M V L A P L P D P G P P S L A V A P E P C P Q P L R S P S L D N P T P F P N L G P S E N P L K R L L V P G E E W E F E V T A F Y R G R Q V F Q Q T I S C P E G L R L V G S E V G D R T L P G W P V T L P D P G M S L T D R G V M S Y V R H V L S C L G G G L A L W R A G Q W L W A Q R L G H C H T Y W A V S E E L L P N S G H G P D G E V P K D K E G G V F D L G P F I V D L I T F T E G S G R S P R Y A L W F C V G E S W P Q D Q P W T K R L V M V K V V P T C L R A L V E M A R V G G A S S L E N T V D L H I S N S H P L S L T S D Q Y K A Y L Q D L V E G M D F Q G P G E S
Full Length
50.3 kDa
2 to 427
Recombinant
His-T7 tag N-Terminus
Liquid
ab205216 was expressed in E.coli as inclusion bodies, refolded using a unique “temperature shift inclusion body refolding” technology, chromatographically purified and sterile-filtered.
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:24049179, PubMed:25636800, PubMed:27302953, PubMed:31340999, PubMed:36603579, PubMed:8524823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995, PubMed:36603579, PubMed:8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148, PubMed:36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Belongs to the IRF family.
Constitutively phosphorylated on many Ser/Thr residues (PubMed:22394562, PubMed:23478265, PubMed:23746807). Activated following phosphorylation by TBK1 and IKBKE (PubMed:23478265, PubMed:23746807, PubMed:25636800, PubMed:36603579). Innate adapter proteins, such as MAVS, STING1 or TICAM1, are first activated by viral RNA, cytosolic DNA, and bacterial lipopolysaccharide (LPS), respectively, leading to activation of the kinases TBK1 and IKBKE (PubMed:25636800). These kinases then phosphorylate the adapter proteins on the pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (PubMed:25636800). Phosphorylation at Ser-386 is followed by pyrophosphorylation at the same residue, promoting phosphorylation at Ser-396 (PubMed:36603579). Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce IFNs (PubMed:25636800, PubMed:36603579).
Nucleus, Mitochondrion
Blue Ice
-80°C
-80°C
This supplementary information is collated from multiple sources and compiled automatically.
IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.
IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.
IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.
IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.
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