Recombinant Human IRF4 protein is a Human Full Length protein, in the 1 to 451 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M N L E G G G R G G E F G M S A V S C G N G K L R Q W L I D Q I D S G K Y P G L V W E N E E K S I F R I P W K H A G K Q D Y N R E E D A A L F K A W A L F K G K F R E G I D K P D P P T W K T R L R C A L N K S N D F E E L V E R S Q L D I S D P Y K V Y R I V P E G A K K G A K Q L T L E D P Q M S M S H P Y T M T T P Y P S L P A Q Q V H N Y M M P P L D R S W R D Y V P D Q P H P E I P Y Q C P M T F G P R G H H W Q G P A C E N G C Q V T G T F Y A C A P P E S Q A P G V P T E P S I R S A E A L A F S D C R L H I C L Y Y R E I L V K E L T T S S P E G C R I S H G H T Y D A S N L D Q V L L P Y P E D N G Q R K N I E K L L S H L E R G V V P W M A P D G L Y A K R L C Q S R I Y W D G P L A L C N D R P N K L E R D Q T C K L F D T Q Q F L S E L Q A F A H H G R S L P R F Q V T L C F G E E F P D P Q R Q R K L I T A H V E P L L A R Q L Y Y F A Q Q N S G H F L R G Y D L P E H I S N P E D Y H R S I R H S S I Q E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
Alternative names
MUM1, IRF4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, NF-EM5, LSIRF
Recommended products
Recombinant Human IRF4 protein is a Human Full Length protein, in the 1 to 451 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q15306-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M N L E G G G R G G E F G M S A V S C G N G K L R Q W L I D Q I D S G K Y P G L V W E N E E K S I F R I P W K H A G K Q D Y N R E E D A A L F K A W A L F K G K F R E G I D K P D P P T W K T R L R C A L N K S N D F E E L V E R S Q L D I S D P Y K V Y R I V P E G A K K G A K Q L T L E D P Q M S M S H P Y T M T T P Y P S L P A Q Q V H N Y M M P P L D R S W R D Y V P D Q P H P E I P Y Q C P M T F G P R G H H W Q G P A C E N G C Q V T G T F Y A C A P P E S Q A P G V P T E P S I R S A E A L A F S D C R L H I C L Y Y R E I L V K E L T T S S P E G C R I S H G H T Y D A S N L D Q V L L P Y P E D N G Q R K N I E K L L S H L E R G V V P W M A P D G L Y A K R L C Q S R I Y W D G P L A L C N D R P N K L E R D Q T C K L F D T Q Q F L S E L Q A F A H H G R S L P R F Q V T L C F G E E F P D P Q R Q R K L I T A H V E P L L A R Q L Y Y F A Q Q N S G H F L R G Y D L P E H I S N P E D Y H R S I R H S S I Q E
- Accession
- Q15306
- Protein length
- Full Length
- Predicted molecular weight
- 75.35 kDa
- Amino acids
- 1 to 451
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
- Sequence similarities
Belongs to the IRF family.
- Post-translational modifications
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.
- Biological function summary
IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.
- Pathways
IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.
- Associated diseases and disorders
IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.
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1 product image
SDS-PAGE - Recombinant Human IRF4 protein (ab132104)
12.5% SDS-PAGE stained with Coomassie Blue showing ab132104.
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