Recombinant Human IRF5 Protein (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS.
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Key facts
- Purity
>=95% HPLC
- Endotoxin level
= 0.005 EU/µg
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
MS
- Biologically active
No
- Mass spectrometry
LC-MS/MS
Amino acid sequence
M N Q S I P V A P T P P R R V R L K P W L V A Q V N S C Q Y P G L Q W V N G E K K L F C I P W R H A T R H G P S Q D G D N T I F K A W A K E T G K Y T E G V D E A D P A K W K A N L R C A L N K S R D F R L I Y D G P R D M P P Q P Y K I Y E V C S N G P A P T D S Q P P E D Y S F G A G E E E E E E E E L Q R M L P S L S L T E D V K W P P T L Q P P T L R P P T L Q P P T L Q P P V V L G P P A P D P S P L A P P P G N P A G F R E L L S E V L E P G P L P A S L P P A G E Q L L P D L L I S P H M L P L T D L E I K F Q Y R G R P P R A L T I S N P H G C R L F Y S Q L E A T Q E Q V E L F G P I S L E Q V R F P S P E D I P S D K Q R F Y T N Q L L D V L D R G L I L Q L Q G Q D L Y A I R L C Q C K V F W S G P C A S A H D S C P N P I Q R E V K T K L F S L E H F L N E L I L F Q K G Q T N T P P P F E I F F C F G E E W P D R K P R E K K L I T V Q V V P V A A R L L L E M F S G E L S W S A D S I R L Q I S N P D L K D R M V E Q F K E L H H I W Q S Q Q R L Q P V A Q A P P G A G L G V G Q G P W P M H P A G M Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:11303025, PubMed:15695821, PubMed:22412986, PubMed:25326418, PubMed:32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
Interferon regulatory factor 5, IRF-5, IRF5
Recommended products
Recombinant Human IRF5 Protein (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS.
Alternative names
Interferon regulatory factor 5, IRF-5, IRF5
Key facts
- Purity
>=95% HPLC
- Endotoxin level
= 0.005 EU/µg
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
MS
- Biologically active
No
- Mass spectrometry
LC-MS/MS
- Accession
- Q13568-1
- Animal free
Yes
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.7293% Sodium chloride, 0.3002% Tris HCl, 0.0148% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M N Q S I P V A P T P P R R V R L K P W L V A Q V N S C Q Y P G L Q W V N G E K K L F C I P W R H A T R H G P S Q D G D N T I F K A W A K E T G K Y T E G V D E A D P A K W K A N L R C A L N K S R D F R L I Y D G P R D M P P Q P Y K I Y E V C S N G P A P T D S Q P P E D Y S F G A G E E E E E E E E L Q R M L P S L S L T E D V K W P P T L Q P P T L R P P T L Q P P T L Q P P V V L G P P A P D P S P L A P P P G N P A G F R E L L S E V L E P G P L P A S L P P A G E Q L L P D L L I S P H M L P L T D L E I K F Q Y R G R P P R A L T I S N P H G C R L F Y S Q L E A T Q E Q V E L F G P I S L E Q V R F P S P E D I P S D K Q R F Y T N Q L L D V L D R G L I L Q L Q G Q D L Y A I R L C Q C K V F W S G P C A S A H D S C P N P I Q R E V K T K L F S L E H F L N E L I L F Q K G Q T N T P P P F E I F F C F G E E W P D R K P R E K K L I T V Q V V P V A A R L L L E M F S G E L S W S A D S I R L Q I S N P D L K D R M V E Q F K E L H H I W Q S Q Q R L Q P V A Q A P P G A G L G V G Q G P W P M H P A G M Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
57.11 kDa
- Actual molecular weight
57.12 kDa
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Liquid
General info
- Function
Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:11303025, PubMed:15695821, PubMed:22412986, PubMed:25326418, PubMed:32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
- Sequence similarities
Belongs to the IRF family.
- Post-translational modifications
Phosphorylation of serine and threonine residues by IKBKB in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/EP300 and initiation of transcription.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
Supplementary info
- Activity summary
IRF5 or Interferon Regulatory Factor 5 is a member of the IRF family of transcription factors. This protein weighs approximately 56 kDa and is expressed in cells of the immune system including B cells dendritic cells and macrophages. IRF5 plays an important role in regulating the transcription of interferon-stimulated genes following a viral infection. It acts by binding to specific DNA sequences initiating transcription processes that are important for the antiviral response.
- Biological function summary
IRF5 influences the immune response through the regulation of cytokine production. It activates genes involved in producing pro-inflammatory cytokines such as TNF-α and IL-6 which are important for clearing viral infections and activating immune cells. IRF5 does not usually form part of a protein complex but it interacts closely with TRAF6 and MyD88 for its activation. Its pivotal role in cytokine production highlights its importance in controlling inflammation.
- Pathways
IRF5 is an important component of the Toll-like receptor (TLR) signaling pathway and plays a role in the MyD88-dependent pathway. In this context IRF5 gets activated by TLRs interacting with MyD88 and TAK1 to enhance the transcription of important genes for immune response. It is also closely connected to the IRF3 protein which shares similar activation pathways and can similarly initiate an antiviral state.
- Associated diseases and disorders
Defects or dysregulation in IRF5 function are linked to autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE IRF5 influences autoantibody production contributing to disease development. It works alongside other regulatory proteins involved in autoimmunity including IFN-α and STAT4 which also play significant roles in modulating immune responses. Understanding IRF5's role in these conditions could present new opportunities for therapeutic interventions using IRF5 inhibitors.
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1 product image
Mass Spectrometry - Recombinant Human IRF5 Protein (His tag) (ab318948)
Mass determination by ESI-TOF.
Predicted MW is 57110 Da (+/- 10 Da by ESI-TOF). Observed MW is 57108.97 Da.
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