Recombinant Human ISCU protein
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human ISCU protein is a Human Full Length protein, in the 35 to 167 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
NIFUN, ISCU, Iron-sulfur cluster assembly enzyme ISCU, NifU-like N-terminal domain-containing protein, NifU-like protein
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human ISCU protein (AB103306)
15% SDS-PAGE analysis of 3μg ab103306.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ISCU protein functions as an important scaffold component in the biosynthesis of iron-sulfur (Fe-S) clusters which are integral for the activity of multiple enzymatic and non-enzymatic systems. Within the mitochondria ISCU forms part of a multi-component complex that includes other scaffold proteins and necessary enzymes such as cysteine desulfurase which provides sulfur for cluster assembly. This complex orchestrates the precise coordination of metal ions and scaffold protein interactions facilitating cluster formation.
Pathways
The iron-sulfur cluster scaffold ISCU is vital in the electron transport chain and the citric acid cycle both of which are central to energy production in cells. ISCU closely interacts with proteins like frataxin which delivers iron to the scaffold and ferredoxin which is involved in electron transfer. These interactions highlight the pathway interdependence that maintains cellular bioenergetics and redox balance.
Specifications
Form
Liquid
Additional notes
ab103306 was purified using conventional chromatography techniques.
General info
Function
Isoform 1. Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled (PubMed : 34824239). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (Probable) (PubMed : 24971490, PubMed : 29576242, PubMed : 30031876, PubMed : 34824239). Exists as two slow interchanging conformational states, a structured (S) and disordered (D) form (PubMed : 23940031). May modulate NFS1 desulfurase activity in a zinc-dependent manner (PubMed : 30031876). Modulates the interaction between FXN and the cysteine desulfurase complex (PubMed : 29576242).. Isoform 2. Cytoplasmic scaffold protein, of the cytoplasmic core iron-sulfur cluster (ISC) assembly complex that provides the structural architecture on which the Fe-S clusters are assembled and may be involved in the cytoplasmic iron-sulfur protein biogenesis.
Sequence similarities
Belongs to the NifU family.
Post-translational modifications
Phosphorylation at Ser-14 is required for ISCU protein stabilization in the cytosol, whereas dephosphorylation of Ser-14, due to the inhibition of mTORC1 (mammalian target of rapamycin complex
- complex, leads to degradation of the precursor form and ultimately to a decrease in the mitochondrial mature form.. Cysteine persulfide is reduced by thiol-containing molecules such as glutathione and L-cysteine.
Subcellular localisation
Mitochondrion
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com