JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB158771

Recombinant Human ISG20 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ISG20 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 181 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

HEM45, ISG20, Interferon-stimulated gene 20 kDa protein, Estrogen-regulated transcript 45 protein, Promyelocytic leukemia nuclear body-associated protein ISG20

1 Images
SDS-PAGE - Recombinant Human ISG20 protein (GST tag N-Terminus) (AB158771)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ISG20 protein (GST tag N-Terminus) (AB158771)

ab158771 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q96AZ6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB249340

Anti-ISG20 antibody [EPR8973] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody [EPR8973] - BSA and Azide free (AB249340)

  • 0
  • 0
Primary Antibodies

AB28179

Anti-TFIIF antibody

ChIP - Anti-TFIIF antibody (AB28179)

  • 5
  • 2
Primary Antibodies

AB197660

Anti-Myozenin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myozenin 1 antibody (AB197660)

  • 0
  • 0
Primary Antibodies

AB198171

Anti-NOC2L antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOC2L antibody (AB198171)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIRPEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDMSGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHSSVEDARATMELYQISQRIRARRGLPRLAVSD","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":181,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96AZ6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ISG20 also known as Interferon Stimulated Gene 20 kDa is an exonuclease enzyme with a molecular weight of around 20 kilodaltons. It degrades single-stranded RNA and DNA primarily involved in antiviral defense by targeting viral RNAs. ISG20 is widely expressed in several human tissues with high expression levels in immune-related cells and tissues such as lymph nodes spleen and blood leukocytes. This protein becomes strongly upregulated in response to interferons linking it to the body's innate immune response.
Biological function summary

ISG20 serves as an antiviral factor by degrading viral genomes to restrict infection. It does not work alone; often it functions as part of a larger antiviral mechanism alongside other interferon-induced proteins like PKR and OAS. Through its RNA-degrading activity ISG20 contributes significantly to controlling the spread of viruses by breaking down the RNA from invading organisms particularly RNA viruses. This makes ISG20 an important player in reducing viral loads during infections.

Pathways

ISG20 is mainly associated with the interferon signaling pathway where it acts downstream to execute immune processes. It interacts with and complements other antiviral proteins such as RNase L which also plays a role in degrading RNA during viral responses. Additionally ISG20 links to the broader regulation of apoptosis and cell death pathways showing how it assists in eliminating infected cells to prevent further viral proliferation.

ISG20 has implications in viral infections such as hepatitis C and influenza. Its role in degrading viral RNA positions it as a potential therapeutic target for mitigating these infections. During hepatitis C infection ISG20 works in conjunction with proteins like IRF9 to modulate immune response and limit virus replication. Researchers study ISG20 for its potential contribution to the severity of flu infections where its activity may influence the immune system's effectiveness in combating the influenza virus.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Interferon-induced antiviral exoribonuclease that acts mainly on single-stranded RNA (PubMed : 11401564, PubMed : 12594219, PubMed : 16033969). Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) (PubMed : 16514659, PubMed : 21036379). Inhibition of several viruses such as chikungunya virus (CHIKV) does not involve the degradation of viral RNAs, but rather the inhibition of translation of viral proteins (By similarity). Exerts a translational control over a large panel of non-self RNA substrates while sparing endogenous transcripts. This activity correlates with the protein's ability to localize in cytoplasmic processing bodies (PubMed : 31600344). May also act as master regulator of over hundred interferon stimulated genes leading to viral genome translation inhibition (By similarity). May play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis (PubMed : 16514659).

Sequence similarities

Belongs to the exonuclease superfamily.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Interferon-induced antiviral exoribonuclease that acts mainly on single-stranded RNA (PubMed : 11401564, PubMed : 12594219, PubMed : 16033969). Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) (PubMed : 16514659, PubMed : 21036379). Inhibition of several viruses such as chikungunya virus (CHIKV) does not involve the degradation of viral RNAs, but rather the inhibition of translation of viral proteins (By similarity). Exerts a translational control over a large panel of non-self RNA substrates while sparing endogenous transcripts. This activity correlates with the protein's ability to localize in cytoplasmic processing bodies (PubMed : 31600344). May also act as master regulator of over hundred interferon stimulated genes leading to viral genome translation inhibition (By similarity). May play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis (PubMed : 16514659).
See full target information ISG20
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com