Recombinant Human IYD protein (denatured) is a Human Fragment protein, in the 24 to 214 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S D R S M E K K K G E P R T R A E A R P W V D E D L K D S S D L H Q A E E D A D E W Q E S E E N V E H I P F S H N H Y P E K E M V K R S Q E F Y E L L N K R R S V R F I S N E Q V P M E V I D N V I R T A G T A P S G A H T E P W T F V V V K D P D V K H K I R K I I E E E E E I N Y M K R M G H R W V T D L K K L R T N W I K E Y L D T A P I L I L I F K Q V H G F A A N G K K K V H Y Y N E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the dehalogenation of halotyrosines such as 3-bromo-L-tyrosine, 3-chloro-L-tyrosine, 3-iodo-L-tyrosine and 3,5-diiodo-L-tyrosine (PubMed:15289438, PubMed:18434651, PubMed:25395621, PubMed:28157283). During thyroid hormone biosynthesis, facilitates iodide salvage by catalysing the oxidative NADPH-dependent deiodination of the halogenated by-products of thyroid hormone production, monoiodotyrosine (L-MIT) and diiodotyrosine (L-DIT) (PubMed:15289438, PubMed:18434651). The scavanged iodide can then reenter the hormone-producing pathways (PubMed:15289438, PubMed:18434651). Acts more efficiently on 3-iodo-L-tyrosine than 3,5-diiodo-L-tyrosine (PubMed:15289438).
Alternative names
C6orf71, DEHAL1, IYD, Iodotyrosine deiodinase 1, IYD-1, Iodotyrosine dehalogenase 1
Recommended products
Recombinant Human IYD protein (denatured) is a Human Fragment protein, in the 24 to 214 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q6PHW0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S D R S M E K K K G E P R T R A E A R P W V D E D L K D S S D L H Q A E E D A D E W Q E S E E N V E H I P F S H N H Y P E K E M V K R S Q E F Y E L L N K R R S V R F I S N E Q V P M E V I D N V I R T A G T A P S G A H T E P W T F V V V K D P D V K H K I R K I I E E E E E I N Y M K R M G H R W V T D L K K L R T N W I K E Y L D T A P I L I L I F K Q V H G F A A N G K K K V H Y Y N E
- Accession
- Q6PHW0
- Protein length
- Fragment
- Predicted molecular weight
- 25.1 kDa
- Amino acids
- 24 to 214
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the dehalogenation of halotyrosines such as 3-bromo-L-tyrosine, 3-chloro-L-tyrosine, 3-iodo-L-tyrosine and 3,5-diiodo-L-tyrosine (PubMed:15289438, PubMed:18434651, PubMed:25395621, PubMed:28157283). During thyroid hormone biosynthesis, facilitates iodide salvage by catalysing the oxidative NADPH-dependent deiodination of the halogenated by-products of thyroid hormone production, monoiodotyrosine (L-MIT) and diiodotyrosine (L-DIT) (PubMed:15289438, PubMed:18434651). The scavanged iodide can then reenter the hormone-producing pathways (PubMed:15289438, PubMed:18434651). Acts more efficiently on 3-iodo-L-tyrosine than 3,5-diiodo-L-tyrosine (PubMed:15289438).
- Sequence similarities
Belongs to the nitroreductase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IYD (iodotyrosine deiodinase) also known as iodotyrosine dehalogenase 1 is an enzyme that catalyzes the deiodination of iodotyrosine residues. With a molecular weight of approximately 35 kDa IYD plays a significant role in iodine conservation in thyroid follicles. The enzyme is expressed mainly in the thyroid gland where it recycles iodine from mono- and diiodotyrosine which are by-products of thyroid hormone synthesis. This recycling is important for maintaining proper iodine homeostasis within the body.
- Biological function summary
This enzyme facilitates the recycling of iodine through its dehalogenation activity enabling efficient use of iodine during thyroid hormone production. IYD functions as a monomer not forming any multi-protein complex which allows it to directly interact with iodinated tyrosines without the need for association with other proteins. By ensuring the availability of iodine IYD supports the synthesis of thyroxine (T4) and triiodothyronine (T3) which are hormones critical to metabolic regulation.
- Pathways
Iodotyrosine deiodinase IYD participates in the thyroid hormone synthesis pathway which involves several critical steps including iodine uptake and incorporation into tyrosines. IYD's relationship with key enzymes such as thyroid peroxidase (TPO) highlights its function within this pathway. TPO catalyzes the iodination of tyrosyl residues to produce T3 and T4 subsequently generating iodotyrosines as intermediates where IYD recovers and recycles the iodine.
- Associated diseases and disorders
Impaired IYD activity relates to conditions like hypothyroidism and goiter stemming from inefficient iodine recycling leading to iodine deficiency. Connections between IYD and other thyroidal proteins such as thyroglobulin highlight potential disease mechanisms. Disruptions in thyroid hormone synthesis due to IYD defects can result in inadequate thyroxine and triiodothyronine levels triggering the endocrine imbalances characteristic of these disorders.
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1 product image
SDS-PAGE - Recombinant Human IYD protein (denatured) (ab177648)
15% SDS-PAGE showing ab177648 (3 μg) at approximately 25.1 kDa.
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