Recombinant Human IZUMO1 protein (His tag) is a Human Fragment protein, in the 1 to 292 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G P H F T L L C A A L A G C L L P A E G C V I C D P S V V L A L K S L E K D Y L P G H L D A K H H K A M M E R V E N A V K D F Q E L S L N E D A Y M G V V D E A T L Q K G S W S L L K D L K R I T D S D V K G D L F V K E L F W M L H L Q K E T F A T Y V A R F Q K E A Y C P N K C G V M L Q T L I W C K N C K K E V H A C R K S Y D C G E R N V E V P Q M E D M I L D C E L N W H Q A S E G L T D Y S F Y R V W G N N T E T L V S K G K E A T L T K P M V G P E D A G S Y R C E L G S V N S S P A T I I N F H V T V L P K M I K E E K P S P N I V T P G E A T T E S S I S L Q P L Q P E K M L A S R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed:36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed:36070373).
Alternative names
Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo, OBF, IZUMO1
Recommended products
Recombinant Human IZUMO1 protein (His tag) is a Human Fragment protein, in the 1 to 292 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8IYV9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M G P H F T L L C A A L A G C L L P A E G C V I C D P S V V L A L K S L E K D Y L P G H L D A K H H K A M M E R V E N A V K D F Q E L S L N E D A Y M G V V D E A T L Q K G S W S L L K D L K R I T D S D V K G D L F V K E L F W M L H L Q K E T F A T Y V A R F Q K E A Y C P N K C G V M L Q T L I W C K N C K K E V H A C R K S Y D C G E R N V E V P Q M E D M I L D C E L N W H Q A S E G L T D Y S F Y R V W G N N T E T L V S K G K E A T L T K P M V G P E D A G S Y R C E L G S V N S S P A T I I N F H V T V L P K M I K E E K P S P N I V T P G E A T T E S S I S L Q P L Q P E K M L A S R
- Accession
- Q8IYV9
- Protein length
- Fragment
- Predicted molecular weight
- 32.1 kDa
- Amino acids
- 1 to 292
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed:36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed:36070373).
- Sequence similarities
Belongs to the Izumo family.
- Post-translational modifications
N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IZUMO1 also known as Immunoglobulin superfamily member 9 is a protein important to the process of fertilization. IZUMO1 has a molecular mass of approximately 37 kDa. It localizes specifically to the acrosomal region of sperm cells. This protein plays a mechanical role in sperm-egg fusion an essential step for successful fertilization allowing sperm cells to recognize and bind to the egg cell membrane. Expression of IZUMO1 is limited to mature sperm assuring its involvement exclusively during fertilization.
- Biological function summary
The protein acts when spermatozoa interact with oocytes. IZUMO1 facilitates the binding through forming a complex with Juno the receptor on the egg surface. This interaction is necessary for the initial contact and fusion between the sperm and egg membranes. The IZUMO1-Juno pairing ensures proper docking and fusion enabling the combination of genetic materials from both gametes. The specificity of this binding prevents cross-species fertilization maintaining species integrity during reproduction.
- Pathways
IZUMO1 has involvement in the fertilization pathway. It works directly in the sperm's fusion process an important part of the reproductive process. IZUMO1 interacts closely with other sperm proteins such as CD9 which play roles in adhesion and fusion events. These pathways incorporate IZUMO1 within the larger reproductive framework where it functions alongside these proteins to ensure successful gamete fusion and genetic transfer.
- Associated diseases and disorders
IZUMO1's functionality connects to issues of infertility. Defects or deficiencies in IZUMO1 expression or function can lead to male infertility due to ineffective sperm-egg fusion. Additionally fertility research identifies Juno as a complementary protein in this context as abnormalities in either protein may disrupt fertilization processes and contribute to reproductive challenges. Understanding IZUMO1 offers insight into diagnostic and therapeutic possibilities for treating infertility cases where sperm-egg membrane fusion is compromised.
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1 product image
SDS-PAGE - Recombinant Human IZUMO1 protein (His tag) (ab276551)
SDS-PAGE analysis of ab276551
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