Recombinant Human IZUMO1 protein (His tag)
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Recombinant Human IZUMO1 protein (His tag) is a Human Fragment protein, in the 1 to 292 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.
View Alternative Names
Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo, OBF, IZUMO1
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human IZUMO1 protein (His tag) (AB276551)
SDS-PAGE analysis of ab276551
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein acts when spermatozoa interact with oocytes. IZUMO1 facilitates the binding through forming a complex with Juno the receptor on the egg surface. This interaction is necessary for the initial contact and fusion between the sperm and egg membranes. The IZUMO1-Juno pairing ensures proper docking and fusion enabling the combination of genetic materials from both gametes. The specificity of this binding prevents cross-species fertilization maintaining species integrity during reproduction.
Pathways
IZUMO1 has involvement in the fertilization pathway. It works directly in the sperm's fusion process an important part of the reproductive process. IZUMO1 interacts closely with other sperm proteins such as CD9 which play roles in adhesion and fusion events. These pathways incorporate IZUMO1 within the larger reproductive framework where it functions alongside these proteins to ensure successful gamete fusion and genetic transfer.
Specifications
Form
Lyophilized
General info
Function
Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1 : IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed : 36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed : 36070373).
Sequence similarities
Belongs to the Izumo family.
Post-translational modifications
N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.. Phosphorylated. The cytoplasmic C-terminus is phosphorylated and undergoes phosphorylation changes during epididymal transit.
Target data
Complementary Products
Product promise
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