Skip to main content

Recombinant Human Jagged1 protein (His tag) is a Human Fragment protein, in the 34 to 1046 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant Human Jagged1 protein (His tag) (AB220536), expandable thumbnail

Key facts

Purity
>92% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Tags
His tag C-Terminus
Applications
SDS-PAGE
Biologically active
No

Amino acid sequence

Q F E L E I L S M Q N V N G E L Q N G N C C G G A R N P G D R K C T R D E C D T Y F K V C L K E Y Q S R V T A G G P C S F G S G S T P V I G G N T F N L K A S R G N D R N R I V L P F S F A W P R S Y T L L V E A W D S S N D T V Q P D S I I E K A S H S G M I N P S R Q W Q T L K Q N T G V A H F E Y Q I R V T C D D Y Y Y G F G C N K F C R P R D D F F G H Y A C D Q N G N K T C M E G W M G P E C N R A I C R Q G C S P K H G S C K L P G D C R C Q Y G W Q G L Y C D K C I P H P G C V H G I C N E P W Q C L C E T N W G G Q L C D K D L N Y C G T H Q P C L N G G T C S N T G P D K Y Q C S C P E G Y S G P N C E I A E H A C L S D P C H N R G S C K E T S L G F E C E C S P G W T G P T C S T N I D D C S P N N C S H G G T C Q D L V N G F K C V C P P Q W T G K T C Q L D A N E C E A K P C V N A K S C K N L I A S Y Y C D C L P G W M G Q N C D I N I N D C L G Q C Q N D A S C R D L V N G Y R C I C P P G Y A G D H C E R D I D E C A S N P C L N G G H C Q N E I N R F Q C L C P T G F S G N L C Q L D I D Y C E P N P C Q N G A Q C Y N R A S D Y F C K C P E D Y E G K N C S H L K D H C R T T P C E V I D S C T V A M A S N D T P E G V R Y I S S N V C G P H G K C K S Q S G G K F T C D C N K G F T G T Y C H E N I N D C E S N P C R N G G T C I D G V N S Y K C I C S D G W E G A Y C E T N I N D C S Q N P C H N G G T C R D L V N D F Y C D C K N G W K G K T C H S R D S Q C D E A T C N N G G T C Y D E G D A F K C M C P G G W E G T T C N I A R N S S C L P N P C H N G G T C V V N G E S F T C V C K E G W E G P I C A Q N T N D C S P H P C Y N S G T C V D G D N W Y R C E C A P G F A G P D C R I N I N E C Q S S P C A F G A T C V D E I N G Y R C V C P P G H S G A K C Q E V S G R P C I T M G S V I P D G A K W D D D C N T C Q C L N G R I A C S K V W C G P R P C L L H K G H S E C P S G Q S C I P I L D D Q C F V H P C T G V G E C R S S S L Q P V K T K C T S D S Y Y Q D N C A N I T F T F N K E M M S P G L T T E H I C S E L R N L N I L K N V S A E Y S I Y I A C E P S P S A N N E I H V A I S A E D I R D D G N P I K E I T D K I I D L V S K R D G N S

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed:18660822, PubMed:20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed:9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

Alternative names

CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1

Recommended products

Recombinant Human Jagged1 protein (His tag) is a Human Fragment protein, in the 34 to 1046 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity
>92% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE
Accession
P78504-1
Animal free
No
Species
Human
Reconstitution
Reconstitute at 400 µg/mL in water
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 95% PBS, 5% Trehalose

Sequence info

Amino acid sequence

Q F E L E I L S M Q N V N G E L Q N G N C C G G A R N P G D R K C T R D E C D T Y F K V C L K E Y Q S R V T A G G P C S F G S G S T P V I G G N T F N L K A S R G N D R N R I V L P F S F A W P R S Y T L L V E A W D S S N D T V Q P D S I I E K A S H S G M I N P S R Q W Q T L K Q N T G V A H F E Y Q I R V T C D D Y Y Y G F G C N K F C R P R D D F F G H Y A C D Q N G N K T C M E G W M G P E C N R A I C R Q G C S P K H G S C K L P G D C R C Q Y G W Q G L Y C D K C I P H P G C V H G I C N E P W Q C L C E T N W G G Q L C D K D L N Y C G T H Q P C L N G G T C S N T G P D K Y Q C S C P E G Y S G P N C E I A E H A C L S D P C H N R G S C K E T S L G F E C E C S P G W T G P T C S T N I D D C S P N N C S H G G T C Q D L V N G F K C V C P P Q W T G K T C Q L D A N E C E A K P C V N A K S C K N L I A S Y Y C D C L P G W M G Q N C D I N I N D C L G Q C Q N D A S C R D L V N G Y R C I C P P G Y A G D H C E R D I D E C A S N P C L N G G H C Q N E I N R F Q C L C P T G F S G N L C Q L D I D Y C E P N P C Q N G A Q C Y N R A S D Y F C K C P E D Y E G K N C S H L K D H C R T T P C E V I D S C T V A M A S N D T P E G V R Y I S S N V C G P H G K C K S Q S G G K F T C D C N K G F T G T Y C H E N I N D C E S N P C R N G G T C I D G V N S Y K C I C S D G W E G A Y C E T N I N D C S Q N P C H N G G T C R D L V N D F Y C D C K N G W K G K T C H S R D S Q C D E A T C N N G G T C Y D E G D A F K C M C P G G W E G T T C N I A R N S S C L P N P C H N G G T C V V N G E S F T C V C K E G W E G P I C A Q N T N D C S P H P C Y N S G T C V D G D N W Y R C E C A P G F A G P D C R I N I N E C Q S S P C A F G A T C V D E I N G Y R C V C P P G H S G A K C Q E V S G R P C I T M G S V I P D G A K W D D D C N T C Q C L N G R I A C S K V W C G P R P C L L H K G H S E C P S G Q S C I P I L D D Q C F V H P C T G V G E C R S S S L Q P V K T K C T S D S Y Y Q D N C A N I T F T F N K E M M S P G L T T E H I C S E L R N L N I L K N V S A E Y S I Y I A C E P S P S A N N E I H V A I S A E D I R D D G N P I K E I T D K I I D L V S K R D G N S
Accession
P78504
Protein length
Fragment
Predicted molecular weight
112.3 kDa
Amino acids
34 to 1046
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Lyophilized

General info

Function

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed:18660822, PubMed:20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed:9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.

Biological function summary

The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.

Pathways

Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.

Associated diseases and disorders

Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human Jagged1 protein (His tag) (ab220536), expandable thumbnail

    SDS-PAGE - Recombinant Human Jagged1 protein (His tag) (ab220536)

    SDS-PAGE analysis of ab220536 stainied overnight with Coomassie Blue.

    DTT-reduced protein migrates as 160-180 kDa in SDS-PAGE due to glycosylation.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com