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Recombinant human JAK1 protein (Active) is a Human Fragment protein, in the 866 to 1154 aa range, expressed in Baculovirus infected Sf9, with >=35% purity and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human JAK1 protein (Active) (AB216194), expandable thumbnail
  • SDS-PAGE - Recombinant human JAK1 protein (Active) (AB216194), expandable thumbnail

Key facts

Purity

>=35% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

D P T H F E K R F L K R I R D L G E G H F G K V E L C R Y D P E G D N T G E Q V A V K S L K P E S G G N H I A D L K K E I E I L R N L Y H E N I V K Y K G I C T E D G G N G I K L I M E F L P S G S L K E Y L P K N K N K I N L K Q Q L K Y A V Q I C K G M D Y L G S R Q Y V H R D L A A R N V L V E S E H Q V K I G D F G L T K A I E T D K E Y Y T V K D D R D S P V F W Y A P E C L M Q S K F Y I A S D V W S F G V T L H E L L T Y C D S D S S P M A L F L K M I G P T H G Q M T V T R L V N T L K E G K R L P C P P N C P D E V Y Q L M R K C W E F Q P S N R T S F Q N L I E G F E A L L K

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952). Kinase partner for the type I interferon receptor IFNAR2 (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins (PubMed:7759950). Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors (PubMed:16239216, PubMed:32750333, PubMed:8232552).

Alternative names

Recommended products

Recombinant human JAK1 protein (Active) is a Human Fragment protein, in the 866 to 1154 aa range, expressed in Baculovirus infected Sf9, with >=35% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>=35% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

Specific Activity: 1.0 pmol/min/μg.


Assay Conditions: Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (Tyr6) using Z-Lyte assay conditions.

Accession
P23458-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Sequence info

Amino acid sequence

D P T H F E K R F L K R I R D L G E G H F G K V E L C R Y D P E G D N T G E Q V A V K S L K P E S G G N H I A D L K K E I E I L R N L Y H E N I V K Y K G I C T E D G G N G I K L I M E F L P S G S L K E Y L P K N K N K I N L K Q Q L K Y A V Q I C K G M D Y L G S R Q Y V H R D L A A R N V L V E S E H Q V K I G D F G L T K A I E T D K E Y Y T V K D D R D S P V F W Y A P E C L M Q S K F Y I A S D V W S F G V T L H E L L T Y C D S D S S P M A L F L K M I G P T H G Q M T V T R L V N T L K E G K R L P C P P N C P D E V Y Q L M R K C W E F Q P S N R T S F Q N L I E G F E A L L K

Accession

P23458

Protein length

Fragment

Predicted molecular weight

59 kDa

Amino acids

866 to 1154

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952). Kinase partner for the type I interferon receptor IFNAR2 (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins (PubMed:7759950). Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors (PubMed:16239216, PubMed:32750333, PubMed:8232552).

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Post-translational modifications

Autophosphorylated (PubMed:7615558). Phosphorylated by TYK2 on tyrosine residues in response to type-I interferon signaling, leading to its activation (PubMed:8232552). Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558). Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling (PubMed:11909529).

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

JAK1 also known as Janus Kinase 1 plays an essential role in transmitting signals within cells. It is a tyrosine kinase enzyme with a molecular weight of approximately 130 kDa. JAK1 is expressed broadly in various tissues and is involved in cytokine receptor signaling. The kinase activity of JAK1 gets activated upon cytokine binding to its respective receptor which leads to phosphorylation events and downstream signaling cascades.

Biological function summary

This enzyme participates significantly in the immune response and hematopoiesis by being part of the JAK-STAT signaling pathway complex. When activated JAK1 phosphorylates specific tyrosine residues on the receptor creating docking sites for STAT proteins which then get phosphorylated by JAK1. This phosphorylation allows STAT proteins to dimerize and translocate to the nucleus affecting gene expression involved in growth survival and differentiation processes.

Pathways

JAK1 is importantly engaged in the JAK-STAT pathway and the Interferon signaling pathway. Within these pathways JAK1 collaborates closely with other proteins like JAK2 TYK2 and STAT proteins. It acts as a mediator of the cellular response to a variety of cytokines and growth factors playing a role in the regulation of immune responses and cell proliferation.

Associated diseases and disorders

Dysregulation of JAK1 associates with autoimmune diseases like rheumatoid arthritis and certain cancers. JAK1 inhibitors have been developed as therapeutic agents for these conditions targeting its kinase activity to modulate aberrant signaling. The interaction between JAK1 and other kinases such as JAK2 and transcription factors like STATs highlights its importance in disease mechanisms providing a target for drugs to manage inflammatory and malignant disorders.

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2 product images

  • Functional Studies - Recombinant human JAK1 protein (Active) (ab216194), expandable thumbnail

    Functional Studies - Recombinant human JAK1 protein (Active) (ab216194)

    Example of specific activity of ab216194.

  • SDS-PAGE - Recombinant human JAK1 protein (Active) (ab216194), expandable thumbnail

    SDS-PAGE - Recombinant human JAK1 protein (Active) (ab216194)

    10% SDS-PAGE with Coomassie staining:

    Lane 1: ab216194

    Lane 2: MW marker

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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