Recombinant human JAK1 protein (Active) is a Human Fragment protein, in the 866 to 1154 aa range, expressed in Baculovirus infected Sf9, with >=35% purity and suitable for SDS-PAGE, FuncS.
>=35% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
D P T H F E K R F L K R I R D L G E G H F G K V E L C R Y D P E G D N T G E Q V A V K S L K P E S G G N H I A D L K K E I E I L R N L Y H E N I V K Y K G I C T E D G G N G I K L I M E F L P S G S L K E Y L P K N K N K I N L K Q Q L K Y A V Q I C K G M D Y L G S R Q Y V H R D L A A R N V L V E S E H Q V K I G D F G L T K A I E T D K E Y Y T V K D D R D S P V F W Y A P E C L M Q S K F Y I A S D V W S F G V T L H E L L T Y C D S D S S P M A L F L K M I G P T H G Q M T V T R L V N T L K E G K R L P C P P N C P D E V Y Q L M R K C W E F Q P S N R T S F Q N L I E G F E A L L K
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952). Kinase partner for the type I interferon receptor IFNAR2 (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins (PubMed:7759950). Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors (PubMed:16239216, PubMed:32750333, PubMed:8232552).
JAK1A, JAK1B, JAK1B, JAK1A, JAK1, Tyrosine-protein kinase JAK1, Janus kinase 1, JAK-1
Recombinant human JAK1 protein (Active) is a Human Fragment protein, in the 866 to 1154 aa range, expressed in Baculovirus infected Sf9, with >=35% purity and suitable for SDS-PAGE, FuncS.
>=35% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
Specific Activity: 1.0 pmol/min/μg.
Assay Conditions: Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (Tyr6) using Z-Lyte assay conditions.
No
Human
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
D P T H F E K R F L K R I R D L G E G H F G K V E L C R Y D P E G D N T G E Q V A V K S L K P E S G G N H I A D L K K E I E I L R N L Y H E N I V K Y K G I C T E D G G N G I K L I M E F L P S G S L K E Y L P K N K N K I N L K Q Q L K Y A V Q I C K G M D Y L G S R Q Y V H R D L A A R N V L V E S E H Q V K I G D F G L T K A I E T D K E Y Y T V K D D R D S P V F W Y A P E C L M Q S K F Y I A S D V W S F G V T L H E L L T Y C D S D S S P M A L F L K M I G P T H G Q M T V T R L V N T L K E G K R L P C P P N C P D E V Y Q L M R K C W E F Q P S N R T S F Q N L I E G F E A L L K
Fragment
59 kDa
866 to 1154
Recombinant
GST tag N-Terminus
Liquid
Affinity purified.
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952). Kinase partner for the type I interferon receptor IFNAR2 (PubMed:16239216, PubMed:28111307, PubMed:32750333, PubMed:7615558, PubMed:8232552). In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins (PubMed:7759950). Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors (PubMed:16239216, PubMed:32750333, PubMed:8232552).
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Autophosphorylated (PubMed:7615558). Phosphorylated by TYK2 on tyrosine residues in response to type-I interferon signaling, leading to its activation (PubMed:8232552). Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558). Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling (PubMed:11909529).
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This supplementary information is collated from multiple sources and compiled automatically.
JAK1 also known as Janus Kinase 1 plays an essential role in transmitting signals within cells. It is a tyrosine kinase enzyme with a molecular weight of approximately 130 kDa. JAK1 is expressed broadly in various tissues and is involved in cytokine receptor signaling. The kinase activity of JAK1 gets activated upon cytokine binding to its respective receptor which leads to phosphorylation events and downstream signaling cascades.
This enzyme participates significantly in the immune response and hematopoiesis by being part of the JAK-STAT signaling pathway complex. When activated JAK1 phosphorylates specific tyrosine residues on the receptor creating docking sites for STAT proteins which then get phosphorylated by JAK1. This phosphorylation allows STAT proteins to dimerize and translocate to the nucleus affecting gene expression involved in growth survival and differentiation processes.
JAK1 is importantly engaged in the JAK-STAT pathway and the Interferon signaling pathway. Within these pathways JAK1 collaborates closely with other proteins like JAK2 TYK2 and STAT proteins. It acts as a mediator of the cellular response to a variety of cytokines and growth factors playing a role in the regulation of immune responses and cell proliferation.
Dysregulation of JAK1 associates with autoimmune diseases like rheumatoid arthritis and certain cancers. JAK1 inhibitors have been developed as therapeutic agents for these conditions targeting its kinase activity to modulate aberrant signaling. The interaction between JAK1 and other kinases such as JAK2 and transcription factors like STATs highlights its importance in disease mechanisms providing a target for drugs to manage inflammatory and malignant disorders.
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Example of specific activity of ab216194.
10% SDS-PAGE with Coomassie staining:
Lane 1: ab216194
Lane 2: MW marker
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