Recombinant human JHDM1D protein (Active) is a Human Fragment protein, in the 2 to 488 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A G A A A A V A A G A A A G A A A A A V S V A A P G R A S A P P P P P P V Y C V C R Q P Y D V N R F M I E C D I C K D W F H G S C V G V E E H H A V D I D L Y H C P N C A V L H G S S L M K K R R N W H R H D Y T E I D D G S K P V Q A G T R T F I K E L R S R V F P S A D E I I I K M H G S Q L T Q R Y L E K H G F D V P I M V P K L D D L G L R L P S P T F S V M D V E R Y V G G D K V I D V I D V A R Q A D S K M T L H N Y V K Y F M N P N R P K V L N V I S L E F S D T K M S E L V E V P D I A K K L S W V E N Y W P D D S V F P K P F V Q K Y C L M G V Q D S Y T D F H I D F G G T S V W Y H V L W G E K I F Y L I K P T D E N L A R Y E S W S S S V T Q S E V F F G D K V D K C Y K C V V K Q G H T L F V P T G W I H A V L T S Q D C M A F G G N F L H N L N I G M Q L R C Y E M E K R L K T P D L F K F P F F E A I C W F V A K N L L E T L K E L R E D G F Q P Q T Y L V Q G V K A L H T A L K L W M K K E L V S E H A F E I P D N V R P G H L I K E L S K V I R A I E E E N G K P V K S Q G I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9', 'Lys-27' and 'Lys-36' (H3K9me2, H3K27me2, H3K36me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code (PubMed:20023638, PubMed:20622853). Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3 (PubMed:20023638). Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate (PubMed:20622853).
Alternative names
JHDM1D, KDM7, KIAA1718, KDM7A, Lysine-specific demethylase 7A, JmjC domain-containing histone demethylation protein 1D, Lysine-specific demethylase 7, [histone H3]-dimethyl-L-lysine9 demethylase 7A
Recommended products
Recombinant human JHDM1D protein (Active) is a Human Fragment protein, in the 2 to 488 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 0.2 pmole/min/μg.
- Accession
- Q6ZMT4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- A G A A A A V A A G A A A G A A A A A V S V A A P G R A S A P P P P P P V Y C V C R Q P Y D V N R F M I E C D I C K D W F H G S C V G V E E H H A V D I D L Y H C P N C A V L H G S S L M K K R R N W H R H D Y T E I D D G S K P V Q A G T R T F I K E L R S R V F P S A D E I I I K M H G S Q L T Q R Y L E K H G F D V P I M V P K L D D L G L R L P S P T F S V M D V E R Y V G G D K V I D V I D V A R Q A D S K M T L H N Y V K Y F M N P N R P K V L N V I S L E F S D T K M S E L V E V P D I A K K L S W V E N Y W P D D S V F P K P F V Q K Y C L M G V Q D S Y T D F H I D F G G T S V W Y H V L W G E K I F Y L I K P T D E N L A R Y E S W S S S V T Q S E V F F G D K V D K C Y K C V V K Q G H T L F V P T G W I H A V L T S Q D C M A F G G N F L H N L N I G M Q L R C Y E M E K R L K T P D L F K F P F F E A I C W F V A K N L L E T L K E L R E D G F Q P Q T Y L V Q G V K A L H T A L K L W M K K E L V S E H A F E I P D N V R P G H L I K E L S K V I R A I E E E N G K P V K S Q G I
- Accession
- Q6ZMT4
- Protein length
- Fragment
- Predicted molecular weight
- 56 kDa
- Amino acids
- 2 to 488
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9', 'Lys-27' and 'Lys-36' (H3K9me2, H3K27me2, H3K36me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code (PubMed:20023638, PubMed:20622853). Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3 (PubMed:20023638). Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate (PubMed:20622853).
- Sequence similarities
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
JHDM1D also known as KDM7A is a histone demethylase enzyme involved in the removal of methyl groups from di- and trimethylated lysine residues on histone tails particularly at H3K9 and H3K27. This action impacts gene expression by altering chromatin structure making it an important player in regulating transcriptional activities. The target has a molecular mass of approximately 129 kDa. JHDM1D is widely expressed in various tissues with notably high expression levels in the brain and testis indicating its importance in these organs.
- Biological function summary
JHDM1D functions as part of a larger protein complex that regulates chromatin remodeling and gene expression. It plays a significant role in epigenetic modification processes influencing cell differentiation and development through its demethylation activity. Such capabilities affect cellular proliferation and lineage specification and may impact immune responses and neurological functions.
- Pathways
Epigenetic regulation and chromatin remodeling see direct involvement from JHDM1D through pathways including the histone modification pathway and Wnt signaling pathway. In these pathways JHDM1D interacts with related proteins like EZH2 which also takes part in chromatin modifications and transcriptional control. This interplay highlights the importance of JHDM1D in fine-tuning gene expression.
- Associated diseases and disorders
Altered JHDM1D function relates to cancer progression and multiple neurological disorders. Dysregulation of this protein may contribute to abnormal cell growth and development as seen in certain cancers. Additionally its interactions with proteins like p53 suggest a potential role in tumor suppression pathways. In neurological disorders JHDM1D's impact on brain-specific gene expression may underline deficits seen in conditions like autism spectrum disorders.
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2 product images
Functional Studies - Recombinant human JHDM1D protein (Active) (ab271568)
Specific activity of ab271568.
SDS-PAGE - Recombinant human JHDM1D protein (Active) (ab271568)
SDS-PAGE analysis of 7 μg ab271568.
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