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AB271568

Recombinant human JHDM1D protein (Active)

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Recombinant human JHDM1D protein (Active) is a Human Fragment protein, in the 2 to 488 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

JHDM1D, KDM7, KIAA1718, KDM7A, Lysine-specific demethylase 7A, JmjC domain-containing histone demethylation protein 1D, Lysine-specific demethylase 7, [histone H3]-dimethyl-L-lysine9 demethylase 7A

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Functional Studies - Recombinant human JHDM1D protein (Active) (AB271568)
  • FuncS

Supplier Data

Functional Studies - Recombinant human JHDM1D protein (Active) (AB271568)

Specific activity of ab271568.

SDS-PAGE - Recombinant human JHDM1D protein (Active) (AB271568)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human JHDM1D protein (Active) (AB271568)

SDS-PAGE analysis of 7 μg ab271568.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.2 pmole/min/μg.

Accession

Q6ZMT4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AGAAAAVAAGAAAGAAAAAVSVAAPGRASAPPPPPPVYCVCRQPYDVNRFMIECDICKDWFHGSCVGVEEHHAVDIDLYHCPNCAVLHGSSLMKKRRNWHRHDYTEIDDGSKPVQAGTRTFIKELRSRVFPSADEIIIKMHGSQLTQRYLEKHGFDVPIMVPKLDDLGLRLPSPTFSVMDVERYVGGDKVIDVIDVARQADSKMTLHNYVKYFMNPNRPKVLNVISLEFSDTKMSELVEVPDIAKKLSWVENYWPDDSVFPKPFVQKYCLMGVQDSYTDFHIDFGGTSVWYHVLWGEKIFYLIKPTDENLARYESWSSSVTQSEVFFGDKVDKCYKCVVKQGHTLFVPTGWIHAVLTSQDCMAFGGNFLHNLNIGMQLRCYEMEKRLKTPDLFKFPFFEAICWFVAKNLLETLKELREDGFQPQTYLVQGVKALHTALKLWMKKELVSEHAFEIPDNVRPGHLIKELSKVIRAIEEENGKPVKSQGI","proteinLength":"Fragment","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":488,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q6ZMT4","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JHDM1D also known as KDM7A is a histone demethylase enzyme involved in the removal of methyl groups from di- and trimethylated lysine residues on histone tails particularly at H3K9 and H3K27. This action impacts gene expression by altering chromatin structure making it an important player in regulating transcriptional activities. The target has a molecular mass of approximately 129 kDa. JHDM1D is widely expressed in various tissues with notably high expression levels in the brain and testis indicating its importance in these organs.
Biological function summary

JHDM1D functions as part of a larger protein complex that regulates chromatin remodeling and gene expression. It plays a significant role in epigenetic modification processes influencing cell differentiation and development through its demethylation activity. Such capabilities affect cellular proliferation and lineage specification and may impact immune responses and neurological functions.

Pathways

Epigenetic regulation and chromatin remodeling see direct involvement from JHDM1D through pathways including the histone modification pathway and Wnt signaling pathway. In these pathways JHDM1D interacts with related proteins like EZH2 which also takes part in chromatin modifications and transcriptional control. This interplay highlights the importance of JHDM1D in fine-tuning gene expression.

Altered JHDM1D function relates to cancer progression and multiple neurological disorders. Dysregulation of this protein may contribute to abnormal cell growth and development as seen in certain cancers. Additionally its interactions with proteins like p53 suggest a potential role in tumor suppression pathways. In neurological disorders JHDM1D's impact on brain-specific gene expression may underline deficits seen in conditions like autism spectrum disorders.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9', 'Lys-27' and 'Lys-36' (H3K9me2, H3K27me2, H3K36me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code (PubMed : 20023638, PubMed : 20622853). Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity : in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3 (PubMed : 20023638). Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate (PubMed : 20622853).

Sequence similarities

Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9', 'Lys-27' and 'Lys-36' (H3K9me2, H3K27me2, H3K36me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code (PubMed : 20023638, PubMed : 20622853). Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity : in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3 (PubMed : 20023638). Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate (PubMed : 20622853).
See full target information KDM7A
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Complementary Products

Primary Antibodies

AB230894

Anti-JHDM1D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JHDM1D antibody (AB230894)

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Cell Lines & Lysates

AB258484

Human KDM7A (JHDM1D) knockout A549 cell lysate

Sanger Sequencing - Human KDM7A (JHDM1D) knockout A549 cell lysate (AB258484)

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Cell Lines & Lysates

AB267033

Human KDM7A (JHDM1D) knockout A549 cell line

Sanger Sequencing - Human KDM7A (JHDM1D) knockout A549 cell line (AB267033)

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