Recombinant Human JNK2 protein
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Recombinant Human JNK2 protein is a Human Full Length protein, in the 1 to 382 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
JNK2, PRKM9, SAPK1A, MAPK9, Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, SAPK1a
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human JNK2 protein (AB130019)
SDS-PAGE analysis of ab130019 at 3μg under reducing condition.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C-Jun N-terminal kinase 2 is key in regulating processes such as cell growth apoptosis and differentiation. JNK2 is part of the MAP kinase signal transduction pathways and forms interactions with several proteins including the scaffolding proteins known as JNK-interacting proteins (JIPs). These complexes help coordinate the response of JNK2 in cellular stress and inflammatory responses. JNK2 is also critical in modulating the expression of genes by activating transcription factors such as c-Jun and ATF2.
Pathways
JNK2 operates within the MAPK signaling pathway by integrating various upstream signals to exert effects on gene expression. JNK2 phosphorylates and activates transcription factors playing an important role in cellular responses to stress. It is closely connected to other proteins within the pathway such as JNK1 and JNK3 together contributing to the complex regulation of stress-induced apoptosis and pro-inflammatory responses. These interactions highlight JNK2's essential function across multiple signaling networks.
Specifications
Form
Liquid
Additional notes
ab130019 was purified using conventional chromatography techniques.
General info
Function
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death (PubMed : 10376527, PubMed : 15805466, PubMed : 17525747, PubMed : 19675674, PubMed : 20595622, PubMed : 21364637, PubMed : 22441692, PubMed : 34048572). Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2 (PubMed : 10376527, PubMed : 15805466, PubMed : 17525747, PubMed : 19675674, PubMed : 20595622, PubMed : 21364637, PubMed : 22441692, PubMed : 34048572). In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 10376527). In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3 (PubMed : 15805466). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1 (PubMed : 17525747, PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells (PubMed : 19290929). Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels (PubMed : 19290929). Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption (PubMed : 20595622). When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway (PubMed : 19675674). Participates also in neurite growth in spiral ganglion neurons (By similarity). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. MAPK9 isoforms display different binding patterns : alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Post-translational modifications
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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