Recombinant human JNK3 protein is a Human Full Length protein, in the 2 to 422 aa range, expressed in Baculovirus infected Sf9, with >=55% purity and suitable for SDS-PAGE, FuncS.
>=55% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
S L H F L Y Y C S E P T L D V K I A F C Q G F D K Q V D V S Y I A K H Y N M S K S K V D N Q F Y S V E V G D S T F T V L K R Y Q N L K P I G S G A Q G I V C A A Y D A V L D R N V A I K K L S R P F Q N Q T H A K R A Y R E L V L M K C V N H K N I I S L L N V F T P Q K T L E E F Q D V Y L V M E L M D A N L C Q V I Q M E L D H E R M S Y L L Y Q M L C G I K H L H S A G I I H R D L K P S N I V V K S D C T L K I L D F G L A R T A G T S F M M T P Y V V T R Y Y R A P E V I L G M G Y K E N V D I W S V G C I M G E M V R H K I L F P G R D Y I D Q W N K V I E Q L G T P C P E F M K K L Q P T V R N Y V E N R P K Y A G L T F P K L F P D S L F P A D S E H N K L K A S Q A R D L L S K M L V I D P A K R I S V D D A L Q H P Y I N V W Y D P A E V E A P P P Q I Y D K Q L D E R E H T I E E W K E L I Y K E V M N S E E K T K N G V V K G Q P S P S A Q V Q Q
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).
JNK3, JNK3A, PRKM10, SAPK1B, PRKM10, SAPK1B, MAPK10, JNK3, JNK3A, Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, MAP kinase p49 3F12, Stress-activated protein kinase 1b, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, SAPK1b
Recombinant human JNK3 protein is a Human Full Length protein, in the 2 to 422 aa range, expressed in Baculovirus infected Sf9, with >=55% purity and suitable for SDS-PAGE, FuncS.
>=55% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
Specific activity 22 pmol/min/μg.
Enzyme reaction was performed in 50 μl kinase reaction buffer (40 mM Tris pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.5 mM DTT) containing 50 μM ATP, 0.2 mg/ml ATF2 and 2-16 ng/μl MAPK10 for 30 minutes at 30°C. The amount of ATP transferred was assayed using ATP quantification reagents.
No
Human
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.4% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.05% Sorbitan monolaurate, ethoxylated
S L H F L Y Y C S E P T L D V K I A F C Q G F D K Q V D V S Y I A K H Y N M S K S K V D N Q F Y S V E V G D S T F T V L K R Y Q N L K P I G S G A Q G I V C A A Y D A V L D R N V A I K K L S R P F Q N Q T H A K R A Y R E L V L M K C V N H K N I I S L L N V F T P Q K T L E E F Q D V Y L V M E L M D A N L C Q V I Q M E L D H E R M S Y L L Y Q M L C G I K H L H S A G I I H R D L K P S N I V V K S D C T L K I L D F G L A R T A G T S F M M T P Y V V T R Y Y R A P E V I L G M G Y K E N V D I W S V G C I M G E M V R H K I L F P G R D Y I D Q W N K V I E Q L G T P C P E F M K K L Q P T V R N Y V E N R P K Y A G L T F P K L F P D S L F P A D S E H N K L K A S Q A R D L L S K M L V I D P A K R I S V D D A L Q H P Y I N V W Y D P A E V E A P P P Q I Y D K Q L D E R E H T I E E W K E L I Y K E V M N S E E K T K N G V V K G Q P S P S A Q V Q Q
Full Length
75 kDa
2 to 422
Recombinant
GST tag N-Terminus
Liquid
Affinity purified.
Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
Nucleus, Mitochondrion
Dry Ice
-80°C
-80°C
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This supplementary information is collated from multiple sources and compiled automatically.
JNK3 also known as c-Jun N-terminal kinase 3 belongs to the mitogen-activated protein kinase (MAPK) family. It has a molecular weight of approximately 48 kDa. This protein is part of the signaling cascade that responds to stress stimuli such as cytokines and ultraviolet irradiation. JNK3 expression occurs mainly in the brain but some reports note its expression in the heart and pancreas as well.
JNK3 performs critical functions in neuronal apoptosis and neurodegeneration. JNK3 is part of the MAPK signaling complex which regulates various cellular activities including gene expression and cell differentiation. Activation of JNK3 leads to the phosphorylation and activation of transcription factors like c-Jun which assist in controlling stress-induced cellular responses.
Queries into MAPK pathways reveal significant involvement of JNK3 in the stress-activated protein kinase (SAPK) pathway. JNK3 interacts with proteins such as ASK1 and MKK4/7 serving as upstream kinases to modulate environmental stress responses. The pathway also relates to other MAPKs like ERK and p38 although they perform distinct functions within the signaling network.
JNK3 has strong connections to neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease. Dysregulation in JNK3 signaling leads to increased neuronal cell death aggravating disease pathology. In Alzheimer's disease JNK3 may interact with tau protein contributing to tau phosphorylation and neurofibrillary tangle formation. In Parkinson's disease JNK3 involvement in dopaminergic neuron apoptosis highlights its potential role in disease progression linking it further to key proteins implicated in neurodegeneration.
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SDS-PAGE analysis of 3 μg ab196055 (lane 1) on 10% SDS-PAGE gel and stained with Coomassie blue. Protein marker -lane 2
Specific activity of ab196055
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