Skip to main content

Recombinant human JNK3 protein is a Human Full Length protein, in the 2 to 422 aa range, expressed in Baculovirus infected Sf9, with >=55% purity and suitable for SDS-PAGE, FuncS.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant human JNK3 protein (AB196055), expandable thumbnail
  • Functional Studies - Recombinant human JNK3 protein (AB196055), expandable thumbnail

Key facts

Purity

>=55% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

S L H F L Y Y C S E P T L D V K I A F C Q G F D K Q V D V S Y I A K H Y N M S K S K V D N Q F Y S V E V G D S T F T V L K R Y Q N L K P I G S G A Q G I V C A A Y D A V L D R N V A I K K L S R P F Q N Q T H A K R A Y R E L V L M K C V N H K N I I S L L N V F T P Q K T L E E F Q D V Y L V M E L M D A N L C Q V I Q M E L D H E R M S Y L L Y Q M L C G I K H L H S A G I I H R D L K P S N I V V K S D C T L K I L D F G L A R T A G T S F M M T P Y V V T R Y Y R A P E V I L G M G Y K E N V D I W S V G C I M G E M V R H K I L F P G R D Y I D Q W N K V I E Q L G T P C P E F M K K L Q P T V R N Y V E N R P K Y A G L T F P K L F P D S L F P A D S E H N K L K A S Q A R D L L S K M L V I D P A K R I S V D D A L Q H P Y I N V W Y D P A E V E A P P P Q I Y D K Q L D E R E H T I E E W K E L I Y K E V M N S E E K T K N G V V K G Q P S P S A Q V Q Q

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).

Alternative names

Recommended products

Recombinant human JNK3 protein is a Human Full Length protein, in the 2 to 422 aa range, expressed in Baculovirus infected Sf9, with >=55% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>=55% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

Specific activity 22 pmol/min/μg.

Enzyme reaction was performed in 50 μl kinase reaction buffer (40 mM Tris pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.5 mM DTT) containing 50 μM ATP, 0.2 mg/ml ATF2 and 2-16 ng/μl MAPK10 for 30 minutes at 30°C. The amount of ATP transferred was assayed using ATP quantification reagents.

Accession
P53779-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.4% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.05% Sorbitan monolaurate, ethoxylated

Sequence info

Amino acid sequence

S L H F L Y Y C S E P T L D V K I A F C Q G F D K Q V D V S Y I A K H Y N M S K S K V D N Q F Y S V E V G D S T F T V L K R Y Q N L K P I G S G A Q G I V C A A Y D A V L D R N V A I K K L S R P F Q N Q T H A K R A Y R E L V L M K C V N H K N I I S L L N V F T P Q K T L E E F Q D V Y L V M E L M D A N L C Q V I Q M E L D H E R M S Y L L Y Q M L C G I K H L H S A G I I H R D L K P S N I V V K S D C T L K I L D F G L A R T A G T S F M M T P Y V V T R Y Y R A P E V I L G M G Y K E N V D I W S V G C I M G E M V R H K I L F P G R D Y I D Q W N K V I E Q L G T P C P E F M K K L Q P T V R N Y V E N R P K Y A G L T F P K L F P D S L F P A D S E H N K L K A S Q A R D L L S K M L V I D P A K R I S V D D A L Q H P Y I N V W Y D P A E V E A P P P Q I Y D K Q L D E R E H T I E E W K E L I Y K E V M N S E E K T K N G V V K G Q P S P S A Q V Q Q

Accession

P53779

Protein length

Full Length

Predicted molecular weight

75 kDa

Amino acids

2 to 422

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Post-translational modifications

Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.

Subcellular localisation

Nucleus, Mitochondrion

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

JNK3 also known as c-Jun N-terminal kinase 3 belongs to the mitogen-activated protein kinase (MAPK) family. It has a molecular weight of approximately 48 kDa. This protein is part of the signaling cascade that responds to stress stimuli such as cytokines and ultraviolet irradiation. JNK3 expression occurs mainly in the brain but some reports note its expression in the heart and pancreas as well.

Biological function summary

JNK3 performs critical functions in neuronal apoptosis and neurodegeneration. JNK3 is part of the MAPK signaling complex which regulates various cellular activities including gene expression and cell differentiation. Activation of JNK3 leads to the phosphorylation and activation of transcription factors like c-Jun which assist in controlling stress-induced cellular responses.

Pathways

Queries into MAPK pathways reveal significant involvement of JNK3 in the stress-activated protein kinase (SAPK) pathway. JNK3 interacts with proteins such as ASK1 and MKK4/7 serving as upstream kinases to modulate environmental stress responses. The pathway also relates to other MAPKs like ERK and p38 although they perform distinct functions within the signaling network.

Associated diseases and disorders

JNK3 has strong connections to neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease. Dysregulation in JNK3 signaling leads to increased neuronal cell death aggravating disease pathology. In Alzheimer's disease JNK3 may interact with tau protein contributing to tau phosphorylation and neurofibrillary tangle formation. In Parkinson's disease JNK3 involvement in dopaminergic neuron apoptosis highlights its potential role in disease progression linking it further to key proteins implicated in neurodegeneration.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant human JNK3 protein (ab196055), expandable thumbnail

    SDS-PAGE - Recombinant human JNK3 protein (ab196055)

    SDS-PAGE analysis of 3 μg ab196055 (lane 1) on 10% SDS-PAGE gel and stained with Coomassie blue. Protein marker -lane 2

  • Functional Studies - Recombinant human JNK3 protein (ab196055), expandable thumbnail

    Functional Studies - Recombinant human JNK3 protein (ab196055)

    Specific activity of ab196055

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com