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AB134897

Recombinant Human JOSD1 protein

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Recombinant Human JOSD1 protein is a Human Full Length protein, in the 1 to 202 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

JSPH1, KIAA0063, JOSD1, Josephin-1, Josephin domain-containing protein 1

1 Images
SDS-PAGE - Recombinant Human JOSD1 protein (AB134897)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human JOSD1 protein (AB134897)

15% SDS-PAGE analysis of 3 μg ab134897.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q15040

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.06% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as JOSD1, Josephin-1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALTNVMGFIMNLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLMSCVPWKGDKAKSESLELPQAAPPQIYHEKQRRELCALHALNNVFQDSNAFTRDTLQEIFQRLSPNTKMPEWIGGESELRKFLKHHLRGKNCELLLVVPEEVEAHQSWRTDV","proteinLength":"Full Length","predictedMolecularWeight":"25.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":202,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15040","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JOSD1 also known as Josephin domain-containing protein 1 functions as a deubiquitinating enzyme. It specifically removes ubiquitin from protein substrates which regulates protein degradation localization and activity in cells. The protein mass is approximately 45 kDa. JOSD1 is expressed in various tissues with significant levels seen in the brain liver and kidney. By modulating the ubiquitination status of substrates JOSD1 influences cellular processes like signal transduction and response to stress.
Biological function summary

This protein modifies the stability and activity of other proteins by removing ubiquitin molecules. It acts as part of a larger deubiquitinase complex which interacts with protein networks to maintain cellular homeostasis. Through its enzyme activity JOSD1 contributes to the regulation of processes such as protein quality control and cell cycle progression.

Pathways

Scientists have placed JOSD1 in the ubiquitin-proteasome system an important pathway responsible for protein turnover in cells. It has connections with other proteins like USP7 and USP9X in this pathway reflecting its coordination role in protein deubiquitination. JOSD1 also participates in the endoplasmic reticulum-associated degradation (ERAD) pathway helping to eliminate misfolded proteins important for maintaining protein folding homeostasis within the cell.

Abnormal activity of JOSD1 can connect to neurodegenerative disorders such as Alzheimer's disease where protein misfolding and aggregation play significant roles. JOSD1's interaction with proteins like tau and amyloid precursor protein suggests its potential to influence disease progression. Additionally research associates JOSD1 with liver cancer where dysregulated ubiquitin signaling may contribute to tumor development and progression.
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Specifications

Form

Liquid

Additional notes

ab134897 is purified using conventional chromatography techniques.

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General info

Function

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.

Post-translational modifications

Monoubiquitinated (By similarity). Ubiquitination activates deubiquitination activity in vitro.

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Product protocols

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Target data

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.
See full target information JOSD1
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Complementary Products

Primary Antibodies

AB118221

Anti-JOSD1 antibody

Western blot - Anti-JOSD1 antibody (AB118221)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB314201

Native Bovine Collagen protein (FITC)

  • 0
  • 0
Primary Antibodies

AB133350

Anti-CD90 / Thy1 antibody [EPR3133]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (AB133350)

  • 4
  • 6

Product promise

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