Recombinant Human Junctional Adhesion Molecule 1/JAM-A protein is a Human Fragment protein, in the 26 to 238 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M L G S V T V H S S E P E V R I P E N N P V K L S C A Y S G F S S P R V E W K F D Q G D T T R L V C Y N N K I T A S Y E D R V T F L P T G I T F K S V T R E D T G T Y T C M V S E E G G N S Y G E V K V K L I V L V P P S K P T V N I P S S A T I G N R A V L T C S E Q D G S P P S E Y T W F K D G I V M P T N P K S T R A F S N S S Y V L N P T T G E L V F D P L S A S D T G E Y S C E A R N G Y G T P M T S N A V R M E A V E R N V G V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840). (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1. (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.
Alternative names
CD321, JAM1, JCAM, UNQ264/PRO301, F11R, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, JAM-1, PAM-1
Recommended products
Recombinant Human Junctional Adhesion Molecule 1/JAM-A protein is a Human Fragment protein, in the 26 to 238 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y624-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M L G S V T V H S S E P E V R I P E N N P V K L S C A Y S G F S S P R V E W K F D Q G D T T R L V C Y N N K I T A S Y E D R V T F L P T G I T F K S V T R E D T G T Y T C M V S E E G G N S Y G E V K V K L I V L V P P S K P T V N I P S S A T I G N R A V L T C S E Q D G S P P S E Y T W F K D G I V M P T N P K S T R A F S N S S Y V L N P T T G E L V F D P L S A S D T G E Y S C E A R N G Y G T P M T S N A V R M E A V E R N V G V
- Accession
- Q9Y624
- Protein length
- Fragment
- Predicted molecular weight
- 25.8 kDa
- Amino acids
- 26 to 238
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab126670 was purified using conventional chromatography.
General info
- Function
Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840).
- Sequence similarities
Belongs to the immunoglobulin superfamily.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.
- Biological function summary
JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.
- Pathways
JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.
- Associated diseases and disorders
JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.
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SDS-PAGE - Recombinant Human Junctional Adhesion Molecule 1/JAM-A protein (ab126670)
15% SDS-PAGE analysis of ab126670 (3μg)
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